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Literature summary for 2.4.1.49 extracted from
- Purification and properties of cellodextrin phosphorylase from Clostridium thermocellum (1994), J. Ferment. Bioeng., 77, 239-242.
No PubMed abstract available
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | enzyme is a good tool for the synthesis of cellodextrins | Acetivibrio thermocellus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | slight inhibition at 1 mM | Acetivibrio thermocellus |  |
| Cd2+ | slight inhibition at 1 mM | Acetivibrio thermocellus | |
| Cu2+ | slight inhibition at 1 mM | Acetivibrio thermocellus | |
| Hg2+ | slight inhibition at 1 mM | Acetivibrio thermocellus | |
| N-ethylmaleimide | 26% remaining activity at 10 mM | Acetivibrio thermocellus | |
| Zn2+ | slight inhibition at 1 mM | Acetivibrio thermocellus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| dithiothreitol | 143fold activation at 10 mM | Acetivibrio thermocellus | |
| Sr2+ | slight activation at 1 mM | Acetivibrio thermocellus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
amino acid composition | Acetivibrio thermocellus |
| additional information | - |
N-terminal amino acid sequence | Acetivibrio thermocellus |
| 105000 | - |
SDS-PAGE | Acetivibrio thermocellus |
| 200000 | - |
gel filtration | Acetivibrio thermocellus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (1,4-beta-D-glucosyl)n + phosphate | Acetivibrio thermocellus | - |
(1,4-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acetivibrio thermocellus | - |
strain ATCC 27405 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Acetivibrio thermocellus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 64.2 | - |
purified enzyme | Acetivibrio thermocellus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (1,4-beta-D-glucosyl)n + phosphate | - |
Acetivibrio thermocellus | (1,4-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate | - |
r | |
| phosphate + cellopentaose | - |
Acetivibrio thermocellus | cellotetraose + glucose 1-phosphate | - |
r | |
| phosphate + cellotetraose | - |
Acetivibrio thermocellus | cellotriose + glucose 1-phosphate | - |
r | |
| phosphate + cellotriose | - |
Acetivibrio thermocellus | cellobiose + glucose 1-phosphate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 105000, SDS-PAGE | Acetivibrio thermocellus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Acetivibrio thermocellus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
pH 7.5, 10 min, stable | Acetivibrio thermocellus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | 37°C, 1 h, stable | Acetivibrio thermocellus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | contains no pyridoxyl 5'-phosphate | Acetivibrio thermocellus |
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