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Literature summary for 2.4.1.49 extracted from
- Purification and properties of beta-1,4-oligoglucan:orthophosphate glucosyltransferase from Clostridium thermocellum (1969), J. Biol. Chem., 244, 457-464.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | not affected by pyridoxal 5'-phosphate, adenine nucleotides, sugar phosphates and D-glucose | Acetivibrio thermocellus | |
| Reducing agents | e.g. cysteine, dithiothreitol, reduced glutathione, absolute requirement | Acetivibrio thermocellus |
General Stability
| General Stability | Organism |
|---|---|
| absolute requirement for a reducing agent | Acetivibrio thermocellus |
| cysteine, 50 mM, stabilizes | Acetivibrio thermocellus |
| DTT, 40 mM, stabilizes | Acetivibrio thermocellus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | not affected by pyridoxal 5'-phosphate, adenine nucleotides, sugar phosphates and D-glucose | Acetivibrio thermocellus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.13 | - |
phosphate | with cellotriose | Acetivibrio thermocellus |  |
| 0.19 | - |
phosphate | with cellotetraose | Acetivibrio thermocellus | |
| 0.24 | - |
phosphate | with cellopentaose | Acetivibrio thermocellus | |
| 0.26 | - |
phosphate | with cellohexaose | Acetivibrio thermocellus | |
| 0.37 | - |
cellohexaose | - |
Acetivibrio thermocellus | |
| 1 | - |
cellotriose | - |
Acetivibrio thermocellus | |
| 1 | - |
cellotetraose | - |
Acetivibrio thermocellus | |
| 1 | - |
cellopentaose | - |
Acetivibrio thermocellus | |
| 1.2 | - |
cellobiose | with glucose 1-phosphate | Acetivibrio thermocellus | |
| 4.7 | - |
glucose 1-phosphate | with cellobiose | Acetivibrio thermocellus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no activation with 1 mM and 10 mM concentration of Mg2+, Mn2+, Ca2+, Zn2+, Sn2+, Al3+, Cu2+, Ni2+, Co2+, Fe2+ | Acetivibrio thermocellus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (1,4-beta-D-glucosyl)n + phosphate | Acetivibrio thermocellus | involved in the catabolism of cellulose | (1,4-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate | - |
r | |
| (1,4-beta-D-glucosyl)n + phosphate | Acetivibrio thermocellus 651 | involved in the catabolism of cellulose | (1,4-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acetivibrio thermocellus | - |
- |
- |
| Acetivibrio thermocellus 651 | - |
- |
- |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| O2-sensitive, enzyme is inactive in the absence of cysteine or DTT | Acetivibrio thermocellus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Acetivibrio thermocellus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 11.33 | - |
partially purified enzyme | Acetivibrio thermocellus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -5°C, at least 4 months | Acetivibrio thermocellus |
| -5°C, crude enzyme preparation, at least 6 months | Acetivibrio thermocellus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (1,4-beta-D-glucosyl)n + phosphate | - |
Acetivibrio thermocellus | (1,4-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate | - |
r | |
| (1,4-beta-D-glucosyl)n + phosphate | involved in the catabolism of cellulose | Acetivibrio thermocellus | (1,4-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate | - |
r | |
| (1,4-beta-D-glucosyl)n + phosphate | - |
Acetivibrio thermocellus 651 | (1,4-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate | - |
r | |
| (1,4-beta-D-glucosyl)n + phosphate | involved in the catabolism of cellulose | Acetivibrio thermocellus 651 | (1,4-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate | - |
r | |
| additional information | no phosphorolysis of cellobiose, cellulose, laminaritriose, melezitose or raffinose. Overview of cellobiose-analogues as glycosyl acceptors | Acetivibrio thermocellus | ? | - |
? | |
| additional information | no phosphorolysis of cellobiose, cellulose, laminaritriose, melezitose or raffinose. Overview of cellobiose-analogues as glycosyl acceptors | Acetivibrio thermocellus 651 | ? | - |
? | |
| phosphate + cellohexaose | - |
Acetivibrio thermocellus | cellopentaose + glucose 1-phosphate | - |
r | |
| phosphate + cellopentaose | - |
Acetivibrio thermocellus | cellotetraose + glucose 1-phosphate | - |
r | |
| phosphate + cellotetraose | - |
Acetivibrio thermocellus | cellotriose + glucose 1-phosphate | - |
r | |
| phosphate + cellotetraose | - |
Acetivibrio thermocellus 651 | cellotriose + glucose 1-phosphate | - |
r | |
| phosphate + cellotriose | - |
Acetivibrio thermocellus | cellobiose + glucose 1-phosphate | - |
r | |
| phosphate + cellotriose | - |
Acetivibrio thermocellus 651 | cellobiose + glucose 1-phosphate | - |
r | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Acetivibrio thermocellus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Acetivibrio thermocellus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 9 | 10% of maximal activity at pH 5.5, 15% of maximal activity at pH 9.0, Tris-acetate buffer | Acetivibrio thermocellus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | contains no pyridoxyl 5'-phosphate | Acetivibrio thermocellus |
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