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Literature summary for 2.4.1.49 extracted from

  • Sheth, K.; Alexander, J.K.
    Purification and properties of beta-1,4-oligoglucan:orthophosphate glucosyltransferase from Clostridium thermocellum (1969), J. Biol. Chem., 244, 457-464.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Reducing agents e.g. cysteine, dithiothreitol, reduced glutathione, absolute requirement Acetivibrio thermocellus
additional information not affected by pyridoxal 5'-phosphate, adenine nucleotides, sugar phosphates and D-glucose Acetivibrio thermocellus

General Stability

General Stability Organism
absolute requirement for a reducing agent Acetivibrio thermocellus
cysteine, 50 mM, stabilizes Acetivibrio thermocellus
DTT, 40 mM, stabilizes Acetivibrio thermocellus

Inhibitors

Inhibitors Comment Organism Structure
additional information not affected by pyridoxal 5'-phosphate, adenine nucleotides, sugar phosphates and D-glucose Acetivibrio thermocellus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.26
-
phosphate with cellohexaose Acetivibrio thermocellus
1
-
cellotriose
-
Acetivibrio thermocellus
1
-
cellotetraose
-
Acetivibrio thermocellus
1
-
cellopentaose
-
Acetivibrio thermocellus
0.37
-
cellohexaose
-
Acetivibrio thermocellus
0.19
-
phosphate with cellotetraose Acetivibrio thermocellus
1.2
-
cellobiose with glucose 1-phosphate Acetivibrio thermocellus
0.13
-
phosphate with cellotriose Acetivibrio thermocellus
4.7
-
glucose 1-phosphate with cellobiose Acetivibrio thermocellus
0.24
-
phosphate with cellopentaose Acetivibrio thermocellus

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no activation with 1 mM and 10 mM concentration of Mg2+, Mn2+, Ca2+, Zn2+, Sn2+, Al3+, Cu2+, Ni2+, Co2+, Fe2+ Acetivibrio thermocellus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(1,4-beta-D-glucosyl)n + phosphate Acetivibrio thermocellus involved in the catabolism of cellulose (1,4-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
-
r
(1,4-beta-D-glucosyl)n + phosphate Acetivibrio thermocellus 651 involved in the catabolism of cellulose (1,4-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
-
r

Organism

Organism UniProt Comment Textmining
Acetivibrio thermocellus
-
-
-
Acetivibrio thermocellus 651
-
-
-

Oxidation Stability

Oxidation Stability Organism
O2-sensitive, enzyme is inactive in the absence of cysteine or DTT Acetivibrio thermocellus

Purification (Commentary)

Purification (Comment) Organism
partial Acetivibrio thermocellus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
11.33
-
partially purified enzyme Acetivibrio thermocellus

Storage Stability

Storage Stability Organism
-5°C, at least 4 months Acetivibrio thermocellus
-5°C, crude enzyme preparation, at least 6 months Acetivibrio thermocellus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no phosphorolysis of cellobiose, cellulose, laminaritriose, melezitose or raffinose. Overview of cellobiose-analogues as glycosyl acceptors Acetivibrio thermocellus ?
-
?
additional information no phosphorolysis of cellobiose, cellulose, laminaritriose, melezitose or raffinose. Overview of cellobiose-analogues as glycosyl acceptors Acetivibrio thermocellus 651 ?
-
?
(1,4-beta-D-glucosyl)n + phosphate
-
Acetivibrio thermocellus (1,4-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
-
r
(1,4-beta-D-glucosyl)n + phosphate involved in the catabolism of cellulose Acetivibrio thermocellus (1,4-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
-
r
(1,4-beta-D-glucosyl)n + phosphate
-
Acetivibrio thermocellus 651 (1,4-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
-
r
(1,4-beta-D-glucosyl)n + phosphate involved in the catabolism of cellulose Acetivibrio thermocellus 651 (1,4-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
-
r
phosphate + cellotriose
-
Acetivibrio thermocellus cellobiose + glucose 1-phosphate
-
r
phosphate + cellotriose
-
Acetivibrio thermocellus 651 cellobiose + glucose 1-phosphate
-
r
phosphate + cellotetraose
-
Acetivibrio thermocellus cellotriose + glucose 1-phosphate
-
r
phosphate + cellotetraose
-
Acetivibrio thermocellus 651 cellotriose + glucose 1-phosphate
-
r
phosphate + cellopentaose
-
Acetivibrio thermocellus cellotetraose + glucose 1-phosphate
-
r
phosphate + cellohexaose
-
Acetivibrio thermocellus cellopentaose + glucose 1-phosphate
-
r

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Acetivibrio thermocellus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Acetivibrio thermocellus

pH Range

pH Minimum pH Maximum Comment Organism
5.5 9 10% of maximal activity at pH 5.5, 15% of maximal activity at pH 9.0, Tris-acetate buffer Acetivibrio thermocellus

Cofactor

Cofactor Comment Organism Structure
additional information contains no pyridoxyl 5'-phosphate Acetivibrio thermocellus