Activating Compound | Comment | Organism | Structure |
---|---|---|---|
phosphatidylcholine | stimulates | Rattus norvegicus | |
phosphatidylethanolamine | stimulates | Rattus norvegicus | |
Phospholipids | addition to incubation mixture containing Triton X-100 increases activity | Rattus norvegicus |
General Stability | Organism |
---|---|
glycerol stabilizes | Rattus norvegicus |
in presence of glycerol the enzyme can be frozen and rethawed several times without appreciable loss of stability | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Detergents | inhibition by excess detergent | Rattus norvegicus | |
DL-sphingosine | - |
Rattus norvegicus | |
Octanoyl-D-threo-p-nitro-1-phenyl-2-amino-1,3-propanediol | - |
Rattus norvegicus | |
Protein inhibitor | from brain, kidney, spleen and liver of rat and other animals | Rattus norvegicus | |
sodium deoxycholate | strong inhibition | Rattus norvegicus | |
sodium taurocholate | strong inhibition | Rattus norvegicus | |
sodium taurodeoxycholate | strong inhibition | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.018 | - |
2-(2-hydroxyacyl)sphingosine | Km value for ceramide is influenced by its dispersion state, in presence of 2 mM Triton S-100 biphasic kinetics are observed with increasing ceramide concentration, yielding two apparent Km values of 0.018 and 0.15 mM | Rattus norvegicus | |
0.027 | 0.034 | UDPgalactose | - |
Rattus norvegicus | |
0.15 | - |
2-(2-hydroxyacyl)sphingosine | Km value for ceramide is influenced by its dispersion state, in presence of 2 mM Triton S-100 biphasic kinetics are observed with increasing ceramide concentration, yielding two apparent Km values of 0.018 and 0.15 mM | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Rattus norvegicus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
400000 | 500000 | lipoprotein complex | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
15-20 days old | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
lipoprotein | - |
Rattus norvegicus |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.002733 | - |
- |
Rattus norvegicus |
Storage Stability | Organism |
---|---|
-20°C, 50% w/v glycerol, 1 month, about 30% loss of activity | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | not: glucosylceramide, lactosylceramide, asialo GM2ganglioside | Rattus norvegicus | ? | - |
? | |
additional information | not: ceramide containing nonhydroxy fatty acids | Rattus norvegicus | ? | - |
? | |
UDPgalactose + 2-(2-hydroxyacyl)sphingosine | i.e. 2-hydroxy fatty acid ceramide | Rattus norvegicus | 1-(beta-D-galactosyl)-2-(2-hydroxyacyl)sphingosine + UDP | cerebroside | ? | |
UDPgalactose + 2-(2-hydroxyacyl)sphingosine | i.e. 2-hydroxy fatty acid ceramide | Rattus norvegicus | 1-(beta-D-galactosyl)-2-(2-hydroxyacyl)sphingosine + UDP | 2-hydroxy fatty acid cerebroside | ? | |
UDPgalactose + 2-(2-hydroxyacyl)sphingosine | i.e. 2-hydroxy fatty acid ceramide | Rattus norvegicus | 1-(beta-D-galactosyl)-2-(2-hydroxyacyl)sphingosine + UDP | i.e. 2-hydroxy fatty acid galactosylceramide | ? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
27 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | 8.2 | - |
Rattus norvegicus |