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Literature summary for 2.4.1.46 extracted from

  • Masuda, S.; Harada, J.; Yokono, M.; Yuzawa, Y.; Shimojima, M.; Murofushi, K.; Tanaka, H.; Masuda, H.; Murakawa, M.; Haraguchi, T.; Kondo, M.; Nishimura, M.; Yuasa, H.; Noguchi, M.; Oh-Oka, H.; Tanaka, A.; Tamiaki, H.; Ohta, H.
    A monogalactosyldiacylglycerol synthase found in the green sulfur bacterium Chlorobaculum tepidum reveals important roles for galactolipids in photosynthesis (2011), Plant Cell, 23, 2644-2658.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
His tagged protein expressed in Escherichia coli BL21(DE3), expressed in Arabidopsis thaliana Chlorobaculum tepidum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0183
-
 UDP-galactose pH 7.8, 30°C Chlorobaculum tepidum

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasmic membrane
-
 Chlorobaculum tepidum
-
-

Organism

Organism UniProt Comment Textmining
Chlorobaculum tepidum Q8KFI2 putative
-

Purification (Commentary)

Purification (Comment) Organism
immobilized metal ion affinity chromatography Chlorobaculum tepidum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0531
-
 purified His tagged protein, not significantly influenced by phosphatidic acid, pH 7.8, 30°C Chlorobaculum tepidum
7.04
-
 membrane fraction of mgdA expressing Escherichia coli, not significantly influenced by phosphatidic acid, pH 7.8, 30°C Chlorobaculum tepidum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-galactose + sn-1,2-dioleoylglycerol
-
 Chlorobaculum tepidum UDP + 3-beta-D-galactosyl-1,2-dioleoyl-sn-glycerol
-
 ?
UDP-galactose + sn-1-oleoyl-sn-2-palmitoyl-glycerol
-
 Chlorobaculum tepidum UDP + 3-beta-D-galactosyl-sn-1-oleoyl-sn-2-palmitoyl-glycerol
-
 ?

Synonyms

Synonyms Comment Organism
MgdA
-
 Chlorobaculum tepidum
monogalactosyldiacylglycerol synthase A
-
 Chlorobaculum tepidum

General Information

General Information Comment Organism
physiological function essential gene, growth impaired in knockdown mutant, role in chlorosome biogenesis Chlorobaculum tepidum