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Literature summary for 2.4.1.41 extracted from

  • Taus, C.; Lucini, C.; Sato, T.; Furukawa, K.; Grabherr, R.; Staudacher, E.
    Expression and characterization of the first snail-derived UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase (2013), Glycoconj. J., 30, 825-833.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Sf9 insect cells Biomphalaria glabrata

Inhibitors

Inhibitors Comment Organism Structure
acetonitrile 10% (v/v) acetonitrile reduces enzyme activity to about 60% Biomphalaria glabrata
Cu2+
-
Biomphalaria glabrata
EDTA no activity in the presence of EDTA Biomphalaria glabrata
Mn2+ inhibitory at concentrations higher than 80 mM Biomphalaria glabrata
additional information addition of 5% (v/v) of acetonitrile or the addition of up to 10% (v/v) methanol or glycerol has no negative effect on enzyme activity Biomphalaria glabrata

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.046
-
UDP-N-acetyl-alpha-D-galactosamine with polypeptide Muc2 as cosubstrate, at pH 6.0 and 37°C Biomphalaria glabrata
0.064
-
polypeptide Muc2 at pH 6.0 and 37°C Biomphalaria glabrata

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ stimulates activity to a lesser extent than Mn2+ Biomphalaria glabrata
Co2+ stimulates activity to a lesser extent than Mn2+ Biomphalaria glabrata
Mg2+ stimulates activity to a lesser extent than Mn2+ Biomphalaria glabrata
Mn2+ best stimulator of activity Biomphalaria glabrata

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
x * 65000, SDS-PAGE Biomphalaria glabrata

Organism

Organism UniProt Comment Textmining
Biomphalaria glabrata
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein the enzyme is O-glycosylated but does not have any putative N-glycosylation sites Biomphalaria glabrata

Storage Stability

Storage Stability Organism
-20 to 22°C, 72 h, no loss of activity Biomphalaria glabrata
37°C, 72 h, more than 90% loss of activity Biomphalaria glabrata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 UDP-N-acetyl-alpha-D-galactosamine + polypeptide Muc1a sequence APPAHGVTSAPDTRPAPGC Biomphalaria glabrata 2 UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide Muc1a
-
?
2 UDP-N-acetyl-alpha-D-galactosamine + polypeptide Muc1a' sequence AHGVTSAPDTR Biomphalaria glabrata 2 UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide Muc1a'
-
?
UDP-N-acetyl-alpha-D-galactosamine + polypeptide Muc2 best acceptor polypeptide substrate with sequence PTTTPITTTTTVTPTPTPTGTQTK Biomphalaria glabrata UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide Muc2
-
?
UDP-N-acetyl-alpha-D-galactosamine + polypeptide Muc5Ac second best acceptor polypeptide with sequence GTTPSPVPTTSTTSAP Biomphalaria glabrata UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide Muc5Ac
-
?

Subunits

Subunits Comment Organism
? x * 65000, SDS-PAGE Biomphalaria glabrata

Synonyms

Synonyms Comment Organism
ppGalNAcT
-
Biomphalaria glabrata
UDP-GalNAc:polypeptide GalNAc transferase
-
Biomphalaria glabrata
UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase
-
Biomphalaria glabrata

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
optimal incubation temperature for short assays up to 2 h Biomphalaria glabrata

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 6.5
-
Biomphalaria glabrata

General Information

General Information Comment Organism
metabolism the enzyme catalyzes the first step in mucin-type O-glycosylation Biomphalaria glabrata