Literature summary for 2.4.1.41 extracted from

Tian, E.; ten Hagen, K.G.
A UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase is required for epithelial tube formation (2007), J. Biol. Chem., 282, 606-614.

Search for more literature for 2.4.1.41

Cloned(Commentary)

Cloned (Comment)	Organism
PGANT35A in pUAST plasmid for trachea-specific, Gal4-iduceable expression in transgenic flies under control of breathless promoter	Drosophila melanogaster

Protein Variants

Protein Variants	Comment	Organism
additional information	development of pgant35A mutant germ line clones arrests to 88% during embryogenesis, does not reach pupation	Drosophila melanogaster
additional information	pgant35A mutant phenotype less severe and 7% rescue of lethality upon trachea-specific expression of PGANT35A wild-type	Drosophila melanogaster
additional information	severity of phenotypes of transgenic flies correlates with molecular and biochemical characterization of the severity of the mutant allele	Drosophila melanogaster
pgant35A-3775	transgenic fly: heterozygous with pgant35A-SF32 or pgant35A-HG8 allele, germ line clone: heterozygous with pgant35A-HG8 or pgant35A-SF32 allele, nonsense mutation results in translational stop and loss of enzymatic activity	Drosophila melanogaster
pgant35A-HG8	transgenic fly: heterozygous with pgant35A-SF32 or pgant35A-3775 phenotype, germ line clone: homozygous or with pgant35A-3775 allele , nonsense mutation results in translational stop and loss of enzymatic activity	Drosophila melanogaster
pgant35A-SF32	transgenic fly: heterozygous with pgant35A-3775 or pgant35A-HG8 phenotype, germ line clone: homozygous or with pgant35A-3775 allele, missense mutation: Arg to Trp, results in diminished activity	Drosophila melanogaster

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
UDP-N-acetyl-D-galactosamine + polypeptide	Drosophila melanogaster	mucin-type O-linked glycosylation of serine or threonine residues	UDP + N-acetyl-D-galactosaminyl-polypeptide	-	?

Organism

Organism	UniProt	Comment	Textmining
Drosophila melanogaster	Q8MVS5	PGANT35A; PGANT35A is an orthologue of mammalian ppGalNTase -T11	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
embryonic tracheal system	dorsal longitudinal tracheal trunks and tracheal transverse connectives at embryonic stages 15-17, loss of PGANT35A activity leads to tracheal defects at embryonic stages 15-17, altered intracellular trafficking, reduction in apical and luminal epithelial marker components, alterations in cell shape, polarity and zona adherens formation, mislocation and disfunction of septate junctions, disruption of paracellular diffusion barrier formation	Drosophila melanogaster	-
hindgut	loss of PGANT35A activity does not alter morphology or staining patterns of epithelial marker components	Drosophila melanogaster	-
salivary gland	loss of PGANT35A activity does not alter morphology or staining patterns of epithelial marker components	Drosophila melanogaster	-
trachea	loss of PGANT35A activity leads to diminished O-glycosylation in epithelial cells of tracheal tube as well as reduced amount of GalNAc alpha-S/T glycans at the apical cell surface	Drosophila melanogaster	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
UDP-alpha-GalNAc + polypeptide	-	Drosophila melanogaster	GalNAc-alpha-polypeptide + UDP	major mucin-type O-glycan in embryonic tracheal system along the apical region and luman of the tracheal tubes O-glycan, detectable by lectins Jacalin, soybean agglutinin and Vicia villosa agglutinin	?
UDP-N-acetyl-D-galactosamine + polypeptide	mucin-type O-linked glycosylation of serine or threonine residues	Drosophila melanogaster	UDP + N-acetyl-D-galactosaminyl-polypeptide	-	?

Synonyms

Synonyms	Comment	Organism
PGANT	-	Drosophila melanogaster
ppGalNAcT	-	Drosophila melanogaster
ppGaNTase	-	Drosophila melanogaster
UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase	-	Drosophila melanogaster

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Release 2023.1 (January 2023)