BRENDA - Enzyme Database show
show all sequences of 2.4.1.41

The lectin domains of polypeptide GalNAc-transferases exhibit carbohydrate-binding specificity for GalNAc: lectin binding to GalNAc-glycopeptide substrates is required for high density GalNAc-O-glycosylation

Wandall, H.H.; Irazoqui, F.; Tarp, M.A.; Bennett, E.P.; Mandel, U.; Takeuchi, H.; Kato, K.; Irimura, T.; Suryanarayanan, G.; Hollingsworth, M.A.; Clausen, H.; Glycobiology 17, 374-387 (2007)

Data extracted from this reference:

Application
Application
Commentary
Organism
molecular biology
enzyme-linked lectin assay (ELLA) as carbohydrate-binding assay; enzyme-linked lectin assay (ELLA) as carbohydrate-binding assay; enzyme-linked lectin assay (ELLA) as carbohydrate-binding assay; enzyme-linked lectin assay (ELLA) as carbohydrate-binding assay
Homo sapiens
Cloned(Commentary)
Commentary
Organism
in pAcGP67-His vector for amplification as bacculovirus in Sf9 cells and subsequent infection of High Five cells; in pAcGP67-His vector for amplification as bacculovirus in Sf9 cells and subsequent infection of High Five cells; in pAcGP67-His vector for amplification as bacculovirus in Sf9 cells and subsequent infection of High Five cells; in pAcGP67-His vector for amplification as bacculovirus in Sf9 cells and subsequent infection of High Five cells
Homo sapiens
Engineering
Amino acid exchange
Commentary
Organism
GalNAc-T2 lectin domain
amino acids 405 to 578 of GalNAc-T2, lower expression level than full-length enzyme
Homo sapiens
GalNAc-T2-D224H
mutation in DXH nucleotide-binding motif, inactive mutant
Homo sapiens
GalNAc-T2-D458H
mutation in CLD motif of alpha repeat, impaired lectin-mediated MUC1 and GalNAc-MUC1 binding, no impaired specific activity, incorporates 1-2 fewer GalNAc residues per substrate molecule in endpoint reactions, no utilization of partially GalNAc-glycosylated peptides, higher KM for IgA hinge-4GalNAc
Homo sapiens
GalNAc-T2-D541A
mutation in CLD motif of gamma repeat
Homo sapiens
GalNAc-T4 lectin domain
amino acids 432 to 571 of GalNAc-T4, lower expression level than full-length enzyme
Homo sapiens
GalNAc-T4 lectin domain-D459H
mutation in CLD motif of alpha repeat
Homo sapiens
GalNAc-T4-D459H
mutation in CLD motif of alpha repeat, impaired lectin-mediated MUC1 and GalNAc-MUC1 binding
Homo sapiens
General Stability
General Stability
Organism
23% loss of specific activity after 12h shaking at 37C
Homo sapiens
25% loss of specific activity after 12 h shaking at 37C
Homo sapiens
31% loss of specific activity after 12h shaking at 37C
Homo sapiens
mutant GalNAc-T2-D458H: 15% loss of specific activity after 12h shaking at 37C
Homo sapiens
mutant GalNAc-T2-D541A: 19% loss of specific activity after 12h shaking at 37C
Homo sapiens
Inhibitors
Inhibitors
Commentary
Organism
Structure
benzyl-alpha-GalNAc
;
Homo sapiens
D-galactose
-
Homo sapiens
EDTA
GalNAc-T4 enzyme and lectin domain; inhibition of GalNAc-T2 enzyme and lectin domain
Homo sapiens
GalNAc
inhibition for GalNAc-T2 lectin domain to bind GalNAc-MUC1; inhibition for GalNAc-T4 lectin domain to bind GalNAc-MUC1
Homo sapiens
methyl-alpha-Gal
-
Homo sapiens
methyl-beta-Gal
-
Homo sapiens
additional information
benzyl or phenyl alpha and beta-GalNAc compounds show higher inhibition for GalNAc-T2 to bind GalNAc-MUC1 than for isoform GalNAc-T4. No inhibition of GalNAc-T2 enzyme and lectin domain by glucose, GlcNAc or mannose; benzyl or phenyl alpha and beta-GalNAc compounds show weaker inhibition for GalNAc-T4 to bind GalNAc-MUC1 than for isoform GalNAc-T2. No inhibition of GalNAc-T4 enzyme and lectin domain by glucose, GlcNAc or mannose
Homo sapiens
o-nitrophenyl-alpha-GalNAc
;
Homo sapiens
o-nitrophenyl-beta-GalNAc
;
Homo sapiens
p-nitrophenyl-alpha-GalNAc
;
Homo sapiens
p-nitrophenyl-beta-GalNAc
;
Homo sapiens
phenyl-alpha-GalNAc
;
Homo sapiens
UDP-alpha-GalNAc
;
Homo sapiens
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.01
-
IgA hinge
+/-0.01 mM, mutant GalNAc-T2-D541A, pH7.4
Homo sapiens
0.01
-
Muc2
+/-0.001 mM, pH7.4
Homo sapiens
0.011
-
UDP-GalNAc
+/-0.002 mM, pH7.4
Homo sapiens
0.013
-
UDP-GalNAc
+/-0.001 mM, mutant GalNAc-T2-D541A, pH7.4
Homo sapiens
0.015
-
IgA hinge
+/-0.002 mM, pH7.4
Homo sapiens
0.018
-
Muc2
+/-0.003 mM, mutant GalNAc-T2-D541A, pH7.4
Homo sapiens
0.02
-
IgA hinge
+/-0.005 mM, mutant GalNAc-T2-D458H, pH7.4
Homo sapiens
0.03
-
UDP-Gal
+/-0.002 mM, pH7.4
Homo sapiens
0.03
-
UDP-GalNAc
+/-0.001 mM, mutant GalNAc-T2-D458H, pH7.4
Homo sapiens
0.036
-
UDP-Gal
+/-0.001 mM, mutant GalNAc-T2-D458H, pH7.4
Homo sapiens
0.041
-
UDP-Gal
+/-0.005 mM, mutant GalNAc-T2-D541A, pH7.4
Homo sapiens
0.062
-
MUC2-6GalNAc
+/-0.007 mM, pH7.4
Homo sapiens
0.066
-
MUC2-6GalNAc
+/-0.010 mM, mutant GalNAc-T2-D541A, pH7.4
Homo sapiens
0.12
-
IgA hinge-4GalNAc
+/-0.01 mM, pH7.4
Homo sapiens
0.15
-
MUC1
+/-0.03 mM, mutant GalNAc-T2-D541A, pH7.4
Homo sapiens
0.25
-
Muc2
+/-0.006 mM, mutant GalNAc-T2-D458H, pH7.4
Homo sapiens
0.26
-
MUC1
+/-0.16 mM, pH7.4
Homo sapiens
0.32
-
IgA hinge-4GalNAc
+/-0.09 mM, mutant GalNAc-T2-D541A, pH7.4
Homo sapiens
0.36
-
MUC1
+/-0.19mM, mutant GalNAc-T2-D458H, pH7.4
Homo sapiens
0.81
-
IgA hinge-4GalNAc
+/-0.2 mM, mutant GalNAc-T2-D458H, pH7.4
Homo sapiens
0.86
-
MUC1-2GalNAc
+/-0.21 mM, mutant GalNAc-T2-D541A, pH7.4
Homo sapiens
0.87
-
MUC1-2GalNAc
+/-0.23 mM, pH7.4
Homo sapiens
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
intracellular
CHO ldlD-MUC1 cells in presence of GalNAc; CHO ldlD-MUC1 cells in presence of GalNAc; CHO ldlD-MUC1 cells in presence of GalNAc: same pattern of biotinylated GalNAc-T2 like Tn (GalNAc-alpha-Ser/Thr); CHO ldlD-MUC1 cells in presence of GalNAc: same pattern of biotinylated GalNAc-T4 and -T4 lectin like Tn (GalNAc-alpha-Ser/Thr); human salivary glands; human salivary glands; human salivary glands: same pattern of biotinylated GalNAc-T2 at Golgi-like supranuclear locations like Tn (GalNAc-alpha-Ser/Thr); human salivary glands: same pattern of biotinylated GalNAc-T4 lectin at Golgi-like supranuclear locations like Tn (GalNAc-alpha-Ser/Thr)
Homo sapiens
5622
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
UDP-N-acetyl-D-galactosamine + polypeptide
Homo sapiens
mucin-type O-linked glycosylation of serine or threonine residues
UDP + N-acetyl-D-galactosaminyl-polypeptide
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
Q10471
GalNAc-T2
-
Homo sapiens
Q10472
GalNAc-T1
-
Homo sapiens
Q8N4A0
GalNAc-T4
-
Homo sapiens
Q8NCW6
GalNAc-T11
-
Purification (Commentary)
Commentary
Organism
purification of His-tagged truncated lectin domain by IMAC, 250 mM imidazole; purification of His-tagged truncated lectin domain by IMAC, 250 mM imidazole; purification of secreted recombinant proteins from cell culture supernatant by Amberlite IRA-95 chromatography followed by SP Sepharose Fast Flow ion-exchange chromatography and concentrated by centrifugation through 10000 Da cut-off filter followed by elution from a PC3.2/3 column; purification of secreted recombinant proteins from cell culture supernatant by Amberlite IRA-95 chromatography followed by SP Sepharose Fast Flow ion-exchange chromatography and concentrated by centrifugation through 10000 Da cut-off filter followed by elution from a PC3.2/3 column; purification of secreted recombinant proteins from cell culture supernatant by Amberlite IRA-95 chromatography followed by SP Sepharose Fast Flow ion-exchange chromatography and concentrated by centrifugation through 10000 Da cut-off filter followed by elution from a PC3.2/3 column; purification of secreted recombinant proteins from cell culture supernatant by Amberlite IRA-95 chromatography followed by SP Sepharose Fast Flow ion-exchange chromatography and concentrated by centrifugation through 10000 Da cut-off filter followed by elution from a PC3.2/3 column
Homo sapiens
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
additional information
-
0.005 +/-0.007 micromol/min/ml, MUC1-2GalNaC by mutant GalNAc-T2-D458H, pH7.4; 0.018 +/-0.003 micromol/min/ml, MUC7, pH7.4; 0.018 +/-0.004 micromol/min/ml, MUC1-b, pH7.4; 0.025 +/-0.008 micromol/min/ml, MUC1-2GalNAC, pH7.4; 0.028 +/-0.011 micromol/min/ml, MUC1-a' by mutant GalNAc-T2-D541A, pH7.4; 0.029 +/-0.007 micromol/min/ml, MUC1-a' by mutant GalNAc-T2-D458H, pH7.4; 0.029 +/-0.008 micromol/min/ml, MUC7, pH7.4; 0.031 +/-0.003 micromol/min/ml, MUC1-a', pH7.4; 0.036 +/-0.003 micromol/min/ml, MUC7, pH7.4; 0.039 +/-0.010 micromol/min/ml, erythropoitin T, pH7.4; 0.046 +/-0.005 micromol/min/ml, MUC7 by mutant GalNAc-T2-D541A, pH7.4; 0.046 +/-0.006 micromol/min/ml, MUC1-2GalNAC, pH7.4; 0.046 +/-0.009 micromol/min/ml, MUC2-6GalNAc by mutant GalNAc-T2-D541A, pH7.4; 0.047 +/-0.011 micromol/min/ml, erythropoitin T by mutant GalNAc-T2-D458H, pH7.4; 0.051 +/-0.006 micromol/min/ml, hCG-beta, pH7.4; 0.053 +/-0.010 micromol/min/ml, MUC7 by mutant GalNAc-T2-D458H, pH7.4; 0.054 +/-0.001 micromol/min/ml, hCG-beta by mutant GalNAc-T2-D458H, pH7.4; 0.055 +/-0.008 micromol/min/ml, MUC1-2GalNaC by mutant GalNAc-T2-D541A, pH7.4; 0.062 +/-0.010 micromol/min/ml, MUC2-6GalNAc, pH7.4; 0.065 +/-0.007 micromol/min/ml, hCG-beta by mutant GalNAc-T2-D541A, pH7.4; 0.066 +/-0.008 micromol/min/ml, erythropoitin T by mutant GalNAc-T2-D541A, pH7.4; 0.074 +/-0.005 micromol/min/ml, erythropoitin T, pH7.4; 0.076 +/-0.003 micromol/min/ml, erythropoitin T, pH7.4; 0.085 +/-0.003 micromol/min/ml, MUC1-a, pH7.4; 0.086 +/-0.007 micromol/min/ml, MUC1-a, pH7.4; 0.087 +/-0.006 micromol/min/ml, MUC1-b', pH7.4; 0.094 +/-0.008 micromol/min/ml, MUC1-b' by mutant GalNAc-T2-D458H, pH7.4; 0.097 +/-0.002 micromol/min/ml, MUC1, pH7.4; 0.108 +/-0.002 micromol/min/ml, OSM fragment, pH7.4; 0.121 +/-0.013 micromol/min/ml, MUC1 by mutant GalNAc-T2-D458H, pH7.4; 0.122 +/-0.007 micromol/min/ml, MUC1, pH7.4; 0.125 +/-0.003 micromol/min/ml, MUC2, pH7.4; 0.142 +/-0.005 micromol/min/ml, IgA hinge by mutant GalNAc-T2-D541A, pH7.4; 0.151 +/-0.012 micromol/min/ml, MUC1 by mutant GalNAc-T2-D541A, pH7.4; 0.158 +/-0.010 micromol/min/ml, MUC1, pH7.4; 0.161 +/-0.005 micromol/min/ml, IgA hinge, pH7.4; 0.164 +/-0.007 micromol/min/ml, MUC2 by mutant GalNAc-T2-D458H, pH7.4; 0.165 +/-0.013 micromol/min/ml, MUC2 by mutant GalNAc-T2-D541A, pH7.4; 0.185 +/-0.006 micromol/min/ml, IgA hinge by mutant GalNAc-T2-D458H, pH7.4; 0.224 +/-0.002 micromol/min/ml, IgA hinge, pH7.4; 0.281 +/-0.013 micromol/min/ml, IgA hinge, pH7.4; 0.283 +/-0.010 micromol/min/ml, MUC2, pH7.4; 128 +/-0.007 micromol/min/ml, MUC1-b' by mutant GalNAc-T2-D541A, pH7.4; 152 +/-0.018 micromol/min/ml, MUC2, pH7.4
Homo sapiens
45
-
+/-13, UDP-GalNAc by mutant GalNAc-T2-D458H, pH7.4
Homo sapiens
46
-
+/-12, UDP-GalNAc by mutant GalNAc-T2-D541A, pH7.4
Homo sapiens
49
-
+/-5, IgA hinge-4GalNAc by mutant GalNAc-T2-D541A, pH7.4
Homo sapiens
56
-
+/-6, UDP-Gal, pH7.4
Homo sapiens
65
-
+/-5, UDP-Gal by mutant GalNAc-T2-D458H, pH7.4
Homo sapiens
69
-
+/-12, UDP-GalNAc, pH7.4
Homo sapiens
72
-
+/-21, MUC1-2GalNAc, pH7.4
Homo sapiens
81
-
+/-1, MUC2, pH7.4
Homo sapiens
83
-
+/-13, IgA hinge-4GalNAc, pH7.4
Homo sapiens
85
-
+/-5, UDP-Gal by mutant GalNAc-T2-D541A, pH7.4
Homo sapiens
94
-
+/-5, MUC1 by mutant GalNAc-T2-D458H, pH7.4
Homo sapiens
97
-
+/-14, MUC1, pH7.4
Homo sapiens
118
-
+/-39, MUC1-2GalNAc by mutant GalNAc-T2-D541A, pH7.4
Homo sapiens
119
-
+/-24, IgA hinge-4GalNAc by mutant GalNAc-T2-D458H, pH7.4
Homo sapiens
143
-
+/-15, MUC1 by mutant GalNAc-T2-D541A, pH7.4
Homo sapiens
145
-
+/-21, IgA hinge by mutant GalNAc-T2-D541A, pH7.4
Homo sapiens
150
-
+/-6, MUC2 by mutant GalNAc-T2-D458H, pH7.4
Homo sapiens
236
-
+/-1, IgA hinge by mutant GalNAc-T2-D458H, pH7.4
Homo sapiens
273
-
+/-12, MUC2 by mutant GalNAc-T2-D541A, pH7.4
Homo sapiens
292
-
+/-18, MUC2-6GalNAc by mutant GalNAc-T2-D541A, pH7.4
Homo sapiens
302
-
+/-17, MUC2-6GalNAc, pH7.4
Homo sapiens
321
-
+/-18, IgA hinge, pH7.4
Homo sapiens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
erythropoietin T + UDP-Gal
-
686971
Homo sapiens
erythropoietin T-nGalNAc + UDP
-
-
-
?
erythropoietin T + UDP-Gal
also catalysed by mutants GalTNAc-T2-D458H and GalTNAc-T2-D541A
686971
Homo sapiens
erythropoietin T-nGalNAc + UDP
-
-
-
?
hCG-beta + UDP-Gal
-
686971
Homo sapiens
hCG-beta-nGalNAc + UDP
-
-
-
?
IgA hinge + UDP-Gal
-
686971
Homo sapiens
IgA hinge-nGal + UDP
-
-
-
?
IgA hinge + UDP-GalNAc
-
686971
Homo sapiens
IgA hinge-nGalNAc + UDP
-
-
-
?
IgA hinge + UDP-GalNAc
GalTNAc-T11 incorporates only two GalNAc residues into IgA hinge peptide
686971
Homo sapiens
IgA hinge-nGalNAc + UDP
-
-
-
?
IgA hinge + UDP-GalNAc
mutant GalNAc-T2-D458H incorporates 1-2 fewer GalNAc residues than wild-type
686971
Homo sapiens
IgA hinge-nGalNAc + UDP
-
-
-
?
IgA hinge-4GalNAc + UDP-GalNAc
-
686971
Homo sapiens
IgA hinge-nGalNAc + UDP
-
-
-
?
additional information
MUC1-2GalNAc, MUC2-6GalNAc, OSM fragments, hCG-beta are not substrates for GalNAc-T11
686971
Homo sapiens
?
-
-
-
-
additional information
MUC1b' and hCG-beta are no substrates for GalNAc-T1
686971
Homo sapiens
?
-
-
-
-
MUC1 + UDP-GalNAc
-
686971
Homo sapiens
MUC1-nGalNAc + UDP
-
-
-
?
MUC1 + UDP-GalNAc
GalNAc-T4 lectin domain does not exhibit selective specificities for peptide sequences, or the density or pattern of GalNAc glycosylation, GalNAc-T4 lectin domain mediates GalNAc-glycopeptide substrate specificity
686971
Homo sapiens
MUC1-nGalNAc + UDP
-
-
-
?
MUC1 + UDP-GalNAc
mutant GalNAc-T2-D458H incorporates 1-2 fewer GalNAc residues than wild-type, GalNAc-T2 lectin domain does not exhibit selective specificities for peptide sequences, or the density or pattern of GalNAc glycosylation
686971
Homo sapiens
MUC1-nGalNAc + UDP
-
-
-
?
MUC1-2GalNAc + UDP-GalNAc
-
686971
Homo sapiens
MUC1-nGalNAc + UDP
-
-
-
?
MUC1a' + UDP-GalNAc
-
686971
Homo sapiens
MUC1a'-nGalNAc + UDP
-
-
-
?
MUC1b' + UDP-GalNAc
-
686971
Homo sapiens
MUC1b'-nGalNAc + UDP
-
-
-
?
MUC2 + UDP-GalNAc
-
686971
Homo sapiens
MUC2-nGalNAc + UDP
-
-
-
?
MUC2-6GalNAc + UDP-GalNAc
-
686971
Homo sapiens
MUC2-nGalNAc + UDP
-
-
-
?
MUC7 + UDP-GalNAc
-
686971
Homo sapiens
MUC7-nGalNAc + UDP
-
-
-
?
OSM fragment + UDP-Gal
-
686971
Homo sapiens
OSM fragment-nGalNAc + UDP
-
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
mucin-type O-linked glycosylation of serine or threonine residues
686971
Homo sapiens
UDP + N-acetyl-D-galactosaminyl-polypeptide
-
-
-
?
IC50 Value
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
additional information
-
EDTA exhibits IC50 higher than 10 in all cases; EDTA exhibits IC50s higher than 10 in all cases; galactose and methyl-alpha/beta-Gal exhibit IC50 higher than 100 mM for GalNAc-T2 enzyme and lectin domain; galactose and methyl-alpha/beta-Gal exhibit IC50s higher than 100 mM for GalNAc-T4 lectin domain; GalNAc-T2 lectin domain exhibits no specificity for anomeric configuration of GalNAc according to simlar IC50s; GalNAc-T4 lectin domain exhibits no specificity for anomeric configuration of GalNAc according to simlar IC50s; glucose derivatives, lactose and UDP exhibit IC50 higher than 100 mM in all cases; glucose derivatives, lactose and UDP exhibit IC50s higher than 100 mM in all cases; UDP-alpha-GalNAc exhibits IC50 higher than 100 mM for GalNAc-T2 lectin domain; UDP exhibits similar inhibitory effects on GalNAc-T2 and mutant GalNAc-T2-D458H
Homo sapiens
additional information
0.5
-
GalNAc-MUC1 binding to biotinylated GalNAc-T4
Homo sapiens
o-nitrophenyl-alpha-GalNAc
0.8
-
GalNAc-MUC1 binding to biotinylated GalNAc-T4
Homo sapiens
p-nitrophenyl-beta-GalNAc
1
-
GalNAc-MUC1 binding to biotinylated GalNAc-T4
Homo sapiens
benzyl-alpha-GalNAc
1
-
GalNAc-MUC1 binding to biotinylated GalNAc-T4
Homo sapiens
GalNAc
1
-
GalNAc-MUC1 binding to biotinylated GalNAc-T4
Homo sapiens
o-nitrophenyl-beta-GalNAc
1
-
GalNAc-MUC1 binding to biotinylated GalNAc-T4
Homo sapiens
phenyl-alpha-GalNAc
1
-
GalNAc-MUC1 binding to biotinylated GalNAc-T4
Homo sapiens
p-nitrophenyl-alpha-GalNAc
5
-
GalNAc-MUC1 binding to biotinylated GalNAc-T4 lectin domain
Homo sapiens
benzyl-alpha-GalNAc
5
-
GalNAc-MUC1 binding to biotinylated GalNAc-T4 lectin domain
Homo sapiens
GalNAc
5
-
GalNAc-MUC1 binding to biotinylated GalNAc-T4 lectin domain
Homo sapiens
o-nitrophenyl-alpha-GalNAc
5
-
GalNAc-MUC1 binding to biotinylated GalNAc-T2; GalNAc-MUC1 binding to biotinylated GalNAc-T4 lectin domain
Homo sapiens
phenyl-alpha-GalNAc
7
-
GalNAc-MUC1 binding to biotinylated GalNAc-T2
Homo sapiens
o-nitrophenyl-alpha-GalNAc
8
-
GalNAc-MUC1 binding to biotinylated GalNAc-T4 lectin domain
Homo sapiens
ortho-nitrophenyl-beta-GalNAc
8
-
GalNAc-MUC1 binding to biotinylated GalNAc-T4 lectin domain
Homo sapiens
p-nitrophenyl-beta-GalNAc
10
-
GalNAc-MUC1 binding to biotinylated GalNAc-T2
Homo sapiens
o-nitrophenyl-beta-GalNAc
10
-
GalNAc-MUC1 binding to biotinylated GalNAc-T4 lectin domain
Homo sapiens
para-nitrophenyl-alpha-GalNAc
10
-
GalNAc-MUC1 binding to biotinylated GalNAc-T2; GalNAc-MUC1 binding to biotinylated GalNAc-T2 lectin domain
Homo sapiens
p-nitrophenyl-alpha-GalNAc
10
-
GalNAc-MUC1 binding to biotinylated GalNAc-T2; GalNAc-MUC1 binding to biotinylated GalNAc-T2 lectin domain
Homo sapiens
p-nitrophenyl-beta-GalNAc
12
-
GalNAc-MUC1 binding to biotinylated GalNAc-T2 lectin domain
Homo sapiens
o-nitrophenyl-alpha-GalNAc
12
-
GalNAc-MUC1 binding to biotinylated GalNAc-T2 lectin domain
Homo sapiens
o-nitrophenyl-beta-GalNAc
15
-
GalNAc-MUC1 binding to biotinylated GalNAc-T2
Homo sapiens
benzyl-alpha-GalNAc
15
-
GalNAc-MUC1 binding to biotinylated GalNAc-T2
Homo sapiens
GalNAc
15
-
GalNAc-MUC1 binding to biotinylated GalNAc-T2 lectin domain
Homo sapiens
phenyl-alpha-GalNAc
20
-
GalNAc-MUC1 binding to biotinylated GalNAc-T2 lectin domain
Homo sapiens
benzyl-alpha-GalNAc
30
-
GalNAc-MUC1 binding to biotinylated GalNAc-T4
Homo sapiens
UDP-alpha-GalNAc
37
-
GalNAc-MUC1 binding to biotinylated GalNAc-T2 lectin domain
Homo sapiens
GalNAc
40
-
GalNAc-MUC1 binding to biotinylated GalNAc-T4
Homo sapiens
methyl-beta-Gal
50
-
GalNAc-MUC1 binding to biotinylated GalNAc-T4
Homo sapiens
D-galactose
50
-
GalNAc-MUC1 binding to biotinylated GalNAc-T4
Homo sapiens
methyl-alpha-Gal
50
-
GalNAc-MUC1 binding to biotinylated GalNAc-T4 lectin domain
Homo sapiens
UDP-alpha-GalNAc
65
-
GalNAc-MUC1 binding to biotinylated GalNAc-T2
Homo sapiens
UDP-alpha-GalNAc
Application (protein specific)
Application
Commentary
Organism
molecular biology
enzyme-linked lectin assay (ELLA) as carbohydrate-binding assay
Homo sapiens
Cloned(Commentary) (protein specific)
Commentary
Organism
in pAcGP67-His vector for amplification as bacculovirus in Sf9 cells and subsequent infection of High Five cells
Homo sapiens
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
GalNAc-T2 lectin domain
amino acids 405 to 578 of GalNAc-T2, lower expression level than full-length enzyme
Homo sapiens
GalNAc-T2-D224H
mutation in DXH nucleotide-binding motif, inactive mutant
Homo sapiens
GalNAc-T2-D458H
mutation in CLD motif of alpha repeat, impaired lectin-mediated MUC1 and GalNAc-MUC1 binding, no impaired specific activity, incorporates 1-2 fewer GalNAc residues per substrate molecule in endpoint reactions, no utilization of partially GalNAc-glycosylated peptides, higher KM for IgA hinge-4GalNAc
Homo sapiens
GalNAc-T2-D541A
mutation in CLD motif of gamma repeat
Homo sapiens
GalNAc-T4 lectin domain
amino acids 432 to 571 of GalNAc-T4, lower expression level than full-length enzyme
Homo sapiens
GalNAc-T4 lectin domain-D459H
mutation in CLD motif of alpha repeat
Homo sapiens
GalNAc-T4-D459H
mutation in CLD motif of alpha repeat, impaired lectin-mediated MUC1 and GalNAc-MUC1 binding
Homo sapiens
General Stability (protein specific)
General Stability
Organism
23% loss of specific activity after 12h shaking at 37C
Homo sapiens
25% loss of specific activity after 12 h shaking at 37C
Homo sapiens
31% loss of specific activity after 12h shaking at 37C
Homo sapiens
mutant GalNAc-T2-D458H: 15% loss of specific activity after 12h shaking at 37C
Homo sapiens
mutant GalNAc-T2-D541A: 19% loss of specific activity after 12h shaking at 37C
Homo sapiens
IC50 Value (protein specific)
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
additional information
-
EDTA exhibits IC50 higher than 10 in all cases; galactose and methyl-alpha/beta-Gal exhibit IC50 higher than 100 mM for GalNAc-T2 enzyme and lectin domain; GalNAc-T2 lectin domain exhibits no specificity for anomeric configuration of GalNAc according to simlar IC50s; glucose derivatives, lactose and UDP exhibit IC50 higher than 100 mM in all cases; UDP-alpha-GalNAc exhibits IC50 higher than 100 mM for GalNAc-T2 lectin domain; UDP exhibits similar inhibitory effects on GalNAc-T2 and mutant GalNAc-T2-D458H
Homo sapiens
additional information
additional information
-
EDTA exhibits IC50s higher than 10 in all cases; galactose and methyl-alpha/beta-Gal exhibit IC50s higher than 100 mM for GalNAc-T4 lectin domain; GalNAc-T4 lectin domain exhibits no specificity for anomeric configuration of GalNAc according to simlar IC50s; glucose derivatives, lactose and UDP exhibit IC50s higher than 100 mM in all cases
Homo sapiens
additional information
0.5
-
GalNAc-MUC1 binding to biotinylated GalNAc-T4
Homo sapiens
o-nitrophenyl-alpha-GalNAc
0.8
-
GalNAc-MUC1 binding to biotinylated GalNAc-T4
Homo sapiens
p-nitrophenyl-beta-GalNAc
1
-
GalNAc-MUC1 binding to biotinylated GalNAc-T4
Homo sapiens
benzyl-alpha-GalNAc
1
-
GalNAc-MUC1 binding to biotinylated GalNAc-T4
Homo sapiens
GalNAc
1
-
GalNAc-MUC1 binding to biotinylated GalNAc-T4
Homo sapiens
o-nitrophenyl-beta-GalNAc
1
-
GalNAc-MUC1 binding to biotinylated GalNAc-T4
Homo sapiens
phenyl-alpha-GalNAc
1
-
GalNAc-MUC1 binding to biotinylated GalNAc-T4
Homo sapiens
p-nitrophenyl-alpha-GalNAc
5
-
GalNAc-MUC1 binding to biotinylated GalNAc-T4 lectin domain
Homo sapiens
benzyl-alpha-GalNAc
5
-
GalNAc-MUC1 binding to biotinylated GalNAc-T4 lectin domain
Homo sapiens
GalNAc
5
-
GalNAc-MUC1 binding to biotinylated GalNAc-T4 lectin domain
Homo sapiens
o-nitrophenyl-alpha-GalNAc
5
-
GalNAc-MUC1 binding to biotinylated GalNAc-T2
Homo sapiens
phenyl-alpha-GalNAc
5
-
GalNAc-MUC1 binding to biotinylated GalNAc-T4 lectin domain
Homo sapiens
phenyl-alpha-GalNAc
7
-
GalNAc-MUC1 binding to biotinylated GalNAc-T2
Homo sapiens
o-nitrophenyl-alpha-GalNAc
8
-
GalNAc-MUC1 binding to biotinylated GalNAc-T4 lectin domain
Homo sapiens
ortho-nitrophenyl-beta-GalNAc
8
-
GalNAc-MUC1 binding to biotinylated GalNAc-T4 lectin domain
Homo sapiens
p-nitrophenyl-beta-GalNAc
10
-
GalNAc-MUC1 binding to biotinylated GalNAc-T2
Homo sapiens
o-nitrophenyl-beta-GalNAc
10
-
GalNAc-MUC1 binding to biotinylated GalNAc-T4 lectin domain
Homo sapiens
para-nitrophenyl-alpha-GalNAc
10
-
GalNAc-MUC1 binding to biotinylated GalNAc-T2; GalNAc-MUC1 binding to biotinylated GalNAc-T2 lectin domain
Homo sapiens
p-nitrophenyl-alpha-GalNAc
10
-
GalNAc-MUC1 binding to biotinylated GalNAc-T2; GalNAc-MUC1 binding to biotinylated GalNAc-T2 lectin domain
Homo sapiens
p-nitrophenyl-beta-GalNAc
12
-
GalNAc-MUC1 binding to biotinylated GalNAc-T2 lectin domain
Homo sapiens
o-nitrophenyl-alpha-GalNAc
12
-
GalNAc-MUC1 binding to biotinylated GalNAc-T2 lectin domain
Homo sapiens
o-nitrophenyl-beta-GalNAc
15
-
GalNAc-MUC1 binding to biotinylated GalNAc-T2
Homo sapiens
benzyl-alpha-GalNAc
15
-
GalNAc-MUC1 binding to biotinylated GalNAc-T2
Homo sapiens
GalNAc
15
-
GalNAc-MUC1 binding to biotinylated GalNAc-T2 lectin domain
Homo sapiens
phenyl-alpha-GalNAc
20
-
GalNAc-MUC1 binding to biotinylated GalNAc-T2 lectin domain
Homo sapiens
benzyl-alpha-GalNAc
30
-
GalNAc-MUC1 binding to biotinylated GalNAc-T4
Homo sapiens
UDP-alpha-GalNAc
37
-
GalNAc-MUC1 binding to biotinylated GalNAc-T2 lectin domain
Homo sapiens
GalNAc
40
-
GalNAc-MUC1 binding to biotinylated GalNAc-T4
Homo sapiens
methyl-beta-Gal
50
-
GalNAc-MUC1 binding to biotinylated GalNAc-T4
Homo sapiens
D-galactose
50
-
GalNAc-MUC1 binding to biotinylated GalNAc-T4
Homo sapiens
methyl-alpha-Gal
50
-
GalNAc-MUC1 binding to biotinylated GalNAc-T4 lectin domain
Homo sapiens
UDP-alpha-GalNAc
65
-
GalNAc-MUC1 binding to biotinylated GalNAc-T2
Homo sapiens
UDP-alpha-GalNAc
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
benzyl-alpha-GalNAc
-
Homo sapiens
D-galactose
-
Homo sapiens
EDTA
inhibition of GalNAc-T2 enzyme and lectin domain
Homo sapiens
EDTA
GalNAc-T4 enzyme and lectin domain
Homo sapiens
GalNAc
inhibition for GalNAc-T2 lectin domain to bind GalNAc-MUC1
Homo sapiens
GalNAc
inhibition for GalNAc-T4 lectin domain to bind GalNAc-MUC1
Homo sapiens
methyl-alpha-Gal
-
Homo sapiens
methyl-beta-Gal
-
Homo sapiens
additional information
benzyl or phenyl alpha and beta-GalNAc compounds show higher inhibition for GalNAc-T2 to bind GalNAc-MUC1 than for isoform GalNAc-T4. No inhibition of GalNAc-T2 enzyme and lectin domain by glucose, GlcNAc or mannose
Homo sapiens
additional information
benzyl or phenyl alpha and beta-GalNAc compounds show weaker inhibition for GalNAc-T4 to bind GalNAc-MUC1 than for isoform GalNAc-T2. No inhibition of GalNAc-T4 enzyme and lectin domain by glucose, GlcNAc or mannose
Homo sapiens
o-nitrophenyl-alpha-GalNAc
-
Homo sapiens
o-nitrophenyl-beta-GalNAc
-
Homo sapiens
p-nitrophenyl-alpha-GalNAc
-
Homo sapiens
p-nitrophenyl-beta-GalNAc
-
Homo sapiens
phenyl-alpha-GalNAc
-
Homo sapiens
UDP-alpha-GalNAc
-
Homo sapiens
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.01
-
IgA hinge
+/-0.01 mM, mutant GalNAc-T2-D541A, pH7.4
Homo sapiens
0.01
-
Muc2
+/-0.001 mM, pH7.4
Homo sapiens
0.011
-
UDP-GalNAc
+/-0.002 mM, pH7.4
Homo sapiens
0.013
-
UDP-GalNAc
+/-0.001 mM, mutant GalNAc-T2-D541A, pH7.4
Homo sapiens
0.015
-
IgA hinge
+/-0.002 mM, pH7.4
Homo sapiens
0.018
-
Muc2
+/-0.003 mM, mutant GalNAc-T2-D541A, pH7.4
Homo sapiens
0.02
-
IgA hinge
+/-0.005 mM, mutant GalNAc-T2-D458H, pH7.4
Homo sapiens
0.03
-
UDP-Gal
+/-0.002 mM, pH7.4
Homo sapiens
0.03
-
UDP-GalNAc
+/-0.001 mM, mutant GalNAc-T2-D458H, pH7.4
Homo sapiens
0.036
-
UDP-Gal
+/-0.001 mM, mutant GalNAc-T2-D458H, pH7.4
Homo sapiens
0.041
-
UDP-Gal
+/-0.005 mM, mutant GalNAc-T2-D541A, pH7.4
Homo sapiens
0.062
-
MUC2-6GalNAc
+/-0.007 mM, pH7.4
Homo sapiens
0.066
-
MUC2-6GalNAc
+/-0.010 mM, mutant GalNAc-T2-D541A, pH7.4
Homo sapiens
0.12
-
IgA hinge-4GalNAc
+/-0.01 mM, pH7.4
Homo sapiens
0.15
-
MUC1
+/-0.03 mM, mutant GalNAc-T2-D541A, pH7.4
Homo sapiens
0.25
-
Muc2
+/-0.006 mM, mutant GalNAc-T2-D458H, pH7.4
Homo sapiens
0.26
-
MUC1
+/-0.16 mM, pH7.4
Homo sapiens
0.32
-
IgA hinge-4GalNAc
+/-0.09 mM, mutant GalNAc-T2-D541A, pH7.4
Homo sapiens
0.36
-
MUC1
+/-0.19mM, mutant GalNAc-T2-D458H, pH7.4
Homo sapiens
0.81
-
IgA hinge-4GalNAc
+/-0.2 mM, mutant GalNAc-T2-D458H, pH7.4
Homo sapiens
0.86
-
MUC1-2GalNAc
+/-0.21 mM, mutant GalNAc-T2-D541A, pH7.4
Homo sapiens
0.87
-
MUC1-2GalNAc
+/-0.23 mM, pH7.4
Homo sapiens
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
intracellular
CHO ldlD-MUC1 cells in presence of GalNAc: same pattern of biotinylated GalNAc-T2 like Tn (GalNAc-alpha-Ser/Thr); human salivary glands: same pattern of biotinylated GalNAc-T2 at Golgi-like supranuclear locations like Tn (GalNAc-alpha-Ser/Thr)
Homo sapiens
5622
-
intracellular
CHO ldlD-MUC1 cells in presence of GalNAc: same pattern of biotinylated GalNAc-T4 and -T4 lectin like Tn (GalNAc-alpha-Ser/Thr); human salivary glands: same pattern of biotinylated GalNAc-T4 lectin at Golgi-like supranuclear locations like Tn (GalNAc-alpha-Ser/Thr)
Homo sapiens
5622
-
intracellular
CHO ldlD-MUC1 cells in presence of GalNAc; human salivary glands
Homo sapiens
5622
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
UDP-N-acetyl-D-galactosamine + polypeptide
Homo sapiens
mucin-type O-linked glycosylation of serine or threonine residues
UDP + N-acetyl-D-galactosaminyl-polypeptide
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
purification of His-tagged truncated lectin domain by IMAC, 250 mM imidazole; purification of secreted recombinant proteins from cell culture supernatant by Amberlite IRA-95 chromatography followed by SP Sepharose Fast Flow ion-exchange chromatography and concentrated by centrifugation through 10000 Da cut-off filter followed by elution from a PC3.2/3 column
Homo sapiens
purification of secreted recombinant proteins from cell culture supernatant by Amberlite IRA-95 chromatography followed by SP Sepharose Fast Flow ion-exchange chromatography and concentrated by centrifugation through 10000 Da cut-off filter followed by elution from a PC3.2/3 column
Homo sapiens
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
additional information
-
0.005 +/-0.007 micromol/min/ml, MUC1-2GalNaC by mutant GalNAc-T2-D458H, pH7.4; 0.028 +/-0.011 micromol/min/ml, MUC1-a' by mutant GalNAc-T2-D541A, pH7.4; 0.029 +/-0.007 micromol/min/ml, MUC1-a' by mutant GalNAc-T2-D458H, pH7.4; 0.029 +/-0.008 micromol/min/ml, MUC7, pH7.4; 0.031 +/-0.003 micromol/min/ml, MUC1-a', pH7.4; 0.039 +/-0.010 micromol/min/ml, erythropoitin T, pH7.4; 0.046 +/-0.005 micromol/min/ml, MUC7 by mutant GalNAc-T2-D541A, pH7.4; 0.046 +/-0.006 micromol/min/ml, MUC1-2GalNAC, pH7.4; 0.046 +/-0.009 micromol/min/ml, MUC2-6GalNAc by mutant GalNAc-T2-D541A, pH7.4; 0.047 +/-0.011 micromol/min/ml, erythropoitin T by mutant GalNAc-T2-D458H, pH7.4; 0.051 +/-0.006 micromol/min/ml, hCG-beta, pH7.4; 0.053 +/-0.010 micromol/min/ml, MUC7 by mutant GalNAc-T2-D458H, pH7.4; 0.054 +/-0.001 micromol/min/ml, hCG-beta by mutant GalNAc-T2-D458H, pH7.4; 0.055 +/-0.008 micromol/min/ml, MUC1-2GalNaC by mutant GalNAc-T2-D541A, pH7.4; 0.062 +/-0.010 micromol/min/ml, MUC2-6GalNAc, pH7.4; 0.065 +/-0.007 micromol/min/ml, hCG-beta by mutant GalNAc-T2-D541A, pH7.4; 0.066 +/-0.008 micromol/min/ml, erythropoitin T by mutant GalNAc-T2-D541A, pH7.4; 0.087 +/-0.006 micromol/min/ml, MUC1-b', pH7.4; 0.094 +/-0.008 micromol/min/ml, MUC1-b' by mutant GalNAc-T2-D458H, pH7.4; 0.121 +/-0.013 micromol/min/ml, MUC1 by mutant GalNAc-T2-D458H, pH7.4; 0.142 +/-0.005 micromol/min/ml, IgA hinge by mutant GalNAc-T2-D541A, pH7.4; 0.151 +/-0.012 micromol/min/ml, MUC1 by mutant GalNAc-T2-D541A, pH7.4; 0.158 +/-0.010 micromol/min/ml, MUC1, pH7.4; 0.161 +/-0.005 micromol/min/ml, IgA hinge, pH7.4; 0.164 +/-0.007 micromol/min/ml, MUC2 by mutant GalNAc-T2-D458H, pH7.4; 0.165 +/-0.013 micromol/min/ml, MUC2 by mutant GalNAc-T2-D541A, pH7.4; 0.185 +/-0.006 micromol/min/ml, IgA hinge by mutant GalNAc-T2-D458H, pH7.4; 128 +/-0.007 micromol/min/ml, MUC1-b' by mutant GalNAc-T2-D541A, pH7.4; 152 +/-0.018 micromol/min/ml, MUC2, pH7.4
Homo sapiens
additional information
-
0.018 +/-0.003 micromol/min/ml, MUC7, pH7.4; 0.018 +/-0.004 micromol/min/ml, MUC1-b, pH7.4; 0.076 +/-0.003 micromol/min/ml, erythropoitin T, pH7.4; 0.085 +/-0.003 micromol/min/ml, MUC1-a, pH7.4; 0.097 +/-0.002 micromol/min/ml, MUC1, pH7.4; 0.125 +/-0.003 micromol/min/ml, MUC2, pH7.4; 0.224 +/-0.002 micromol/min/ml, IgA hinge, pH7.4
Homo sapiens
additional information
-
0.025 +/-0.008 micromol/min/ml, MUC1-2GalNAC, pH7.4; 0.036 +/-0.003 micromol/min/ml, MUC7, pH7.4; 0.074 +/-0.005 micromol/min/ml, erythropoitin T, pH7.4; 0.086 +/-0.007 micromol/min/ml, MUC1-a, pH7.4; 0.108 +/-0.002 micromol/min/ml, OSM fragment, pH7.4; 0.122 +/-0.007 micromol/min/ml, MUC1, pH7.4; 0.281 +/-0.013 micromol/min/ml, IgA hinge, pH7.4; 0.283 +/-0.010 micromol/min/ml, MUC2, pH7.4
Homo sapiens
45
-
+/-13, UDP-GalNAc by mutant GalNAc-T2-D458H, pH7.4
Homo sapiens
46
-
+/-12, UDP-GalNAc by mutant GalNAc-T2-D541A, pH7.4
Homo sapiens
49
-
+/-5, IgA hinge-4GalNAc by mutant GalNAc-T2-D541A, pH7.4
Homo sapiens
56
-
+/-6, UDP-Gal, pH7.4
Homo sapiens
65
-
+/-5, UDP-Gal by mutant GalNAc-T2-D458H, pH7.4
Homo sapiens
69
-
+/-12, UDP-GalNAc, pH7.4
Homo sapiens
72
-
+/-21, MUC1-2GalNAc, pH7.4
Homo sapiens
81
-
+/-1, MUC2, pH7.4
Homo sapiens
83
-
+/-13, IgA hinge-4GalNAc, pH7.4
Homo sapiens
85
-
+/-5, UDP-Gal by mutant GalNAc-T2-D541A, pH7.4
Homo sapiens
94
-
+/-5, MUC1 by mutant GalNAc-T2-D458H, pH7.4
Homo sapiens
97
-
+/-14, MUC1, pH7.4
Homo sapiens
118
-
+/-39, MUC1-2GalNAc by mutant GalNAc-T2-D541A, pH7.4
Homo sapiens
119
-
+/-24, IgA hinge-4GalNAc by mutant GalNAc-T2-D458H, pH7.4
Homo sapiens
143
-
+/-15, MUC1 by mutant GalNAc-T2-D541A, pH7.4
Homo sapiens
145
-
+/-21, IgA hinge by mutant GalNAc-T2-D541A, pH7.4
Homo sapiens
150
-
+/-6, MUC2 by mutant GalNAc-T2-D458H, pH7.4
Homo sapiens
236
-
+/-1, IgA hinge by mutant GalNAc-T2-D458H, pH7.4
Homo sapiens
273
-
+/-12, MUC2 by mutant GalNAc-T2-D541A, pH7.4
Homo sapiens
292
-
+/-18, MUC2-6GalNAc by mutant GalNAc-T2-D541A, pH7.4
Homo sapiens
302
-
+/-17, MUC2-6GalNAc, pH7.4
Homo sapiens
321
-
+/-18, IgA hinge, pH7.4
Homo sapiens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
erythropoietin T + UDP-Gal
-
686971
Homo sapiens
erythropoietin T-nGalNAc + UDP
-
-
-
?
erythropoietin T + UDP-Gal
also catalysed by mutants GalTNAc-T2-D458H and GalTNAc-T2-D541A
686971
Homo sapiens
erythropoietin T-nGalNAc + UDP
-
-
-
?
hCG-beta + UDP-Gal
-
686971
Homo sapiens
hCG-beta-nGalNAc + UDP
-
-
-
?
IgA hinge + UDP-Gal
-
686971
Homo sapiens
IgA hinge-nGal + UDP
-
-
-
?
IgA hinge + UDP-GalNAc
-
686971
Homo sapiens
IgA hinge-nGalNAc + UDP
-
-
-
?
IgA hinge + UDP-GalNAc
GalTNAc-T11 incorporates only two GalNAc residues into IgA hinge peptide
686971
Homo sapiens
IgA hinge-nGalNAc + UDP
-
-
-
?
IgA hinge + UDP-GalNAc
mutant GalNAc-T2-D458H incorporates 1-2 fewer GalNAc residues than wild-type
686971
Homo sapiens
IgA hinge-nGalNAc + UDP
-
-
-
?
IgA hinge-4GalNAc + UDP-GalNAc
-
686971
Homo sapiens
IgA hinge-nGalNAc + UDP
-
-
-
?
additional information
MUC1-2GalNAc, MUC2-6GalNAc, OSM fragments, hCG-beta are not substrates for GalNAc-T11
686971
Homo sapiens
?
-
-
-
-
additional information
MUC1b' and hCG-beta are no substrates for GalNAc-T1
686971
Homo sapiens
?
-
-
-
-
MUC1 + UDP-GalNAc
-
686971
Homo sapiens
MUC1-nGalNAc + UDP
-
-
-
?
MUC1 + UDP-GalNAc
GalNAc-T4 lectin domain does not exhibit selective specificities for peptide sequences, or the density or pattern of GalNAc glycosylation, GalNAc-T4 lectin domain mediates GalNAc-glycopeptide substrate specificity
686971
Homo sapiens
MUC1-nGalNAc + UDP
-
-
-
?
MUC1 + UDP-GalNAc
mutant GalNAc-T2-D458H incorporates 1-2 fewer GalNAc residues than wild-type, GalNAc-T2 lectin domain does not exhibit selective specificities for peptide sequences, or the density or pattern of GalNAc glycosylation
686971
Homo sapiens
MUC1-nGalNAc + UDP
-
-
-
?
MUC1-2GalNAc + UDP-GalNAc
-
686971
Homo sapiens
MUC1-nGalNAc + UDP
-
-
-
?
MUC1a' + UDP-GalNAc
-
686971
Homo sapiens
MUC1a'-nGalNAc + UDP
-
-
-
?
MUC1b' + UDP-GalNAc
-
686971
Homo sapiens
MUC1b'-nGalNAc + UDP
-
-
-
?
MUC2 + UDP-GalNAc
-
686971
Homo sapiens
MUC2-nGalNAc + UDP
-
-
-
?
MUC2-6GalNAc + UDP-GalNAc
-
686971
Homo sapiens
MUC2-nGalNAc + UDP
-
-
-
?
MUC7 + UDP-GalNAc
-
686971
Homo sapiens
MUC7-nGalNAc + UDP
-
-
-
?
OSM fragment + UDP-Gal
-
686971
Homo sapiens
OSM fragment-nGalNAc + UDP
-
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
mucin-type O-linked glycosylation of serine or threonine residues
686971
Homo sapiens
UDP + N-acetyl-D-galactosaminyl-polypeptide
-
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-
?
Other publictions for EC 2.4.1.41
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
735979
Voglmeir
Biological and biochemical pro ...
Xenopus laevis
Comp. Biochem. Physiol. B
180
40-47
2015
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736203
Kong
Probing polypeptide GalNAc-tra ...
Homo sapiens
Glycobiology
25
55-65
2015
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735431
Lira-Navarrete
Substrate-guided front-face re ...
Homo sapiens
Angew. Chem. Int. Ed. Engl.
53
8206-8210
2014
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736211
Taus
UDP-N-acetyl-alpha-D-galactosa ...
Biomphalaria glabrata
Glycoconj. J.
31
661-670
2014
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736460
Song
Development of isoform-specifi ...
Homo sapiens
J. Biol. Chem.
289
30556-30566
2014
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736918
Wang
Role of the polypeptide N-acet ...
Homo sapiens
Oncotarget
5
544-560
2014
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736929
Gomez
A computational and experiment ...
Homo sapiens
Org. Biomol. Chem.
12
2645-2655
2014
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735737
Irazoqui
An acetylation site in lectin ...
Homo sapiens
Biol. Chem.
394
69-77
2013
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736207
Taus
Expression and characterizatio ...
Biomphalaria glabrata
Glycoconj. J.
30
825-833
2013
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736418
Gerken
The lectin domain of the polyp ...
Bos taurus, Homo sapiens
J. Biol. Chem.
288
19900-19914
2013
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736774
Bhat
Identification of a family of ...
Cryptosporidium hominis, Cryptosporidium muris, Cryptosporidium parvum
Mol. Biochem. Parasitol.
191
24-27
2013
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736913
Wang
The mucin-type glycosylating e ...
Homo sapiens
Oncol. Rep.
30
667-676
2013
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737277
Mochizuki
Expression of polypeptide N-ac ...
Homo sapiens
Thyroid
23
1553-1560
2013
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718991
Schjoldager
Site-specific protein O-glycos ...
Homo sapiens
Biochim. Biophys. Acta
1820
2079-2094
2012
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719568
Yoshimura
Elucidation of the sugar recog ...
Homo sapiens
Glycobiology
22
429-438
2012
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719569
Li
Characterization of ppGalNAc-T ...
Homo sapiens
Glycobiology
22
602-615
2012
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719570
Raman
UDP-N-acetyl-alpha-D-galactosa ...
Homo sapiens
Glycobiology
22
768-777
2012
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17
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719613
Hua
Polypeptide N-acetylgalactosam ...
Homo sapiens
Int. J. Mol. Med.
30
1267-1274
2012
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720946
Schjoldager
Probing isoform-specific funct ...
Homo sapiens
Proc. Natl. Acad. Sci. USA
109
9893-9898
2012
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718747
Zlocowski
Catalytic and glycan-binding a ...
Homo sapiens
Biochem. Biophys. Res. Commun.
410
140-145
2011
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720549
Park
Polypeptide N-acetylgalactosam ...
Homo sapiens
Neoplasia
13
320-326
2011
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704654
Miwa
Isoform-specific O-glycosylati ...
Mus musculus
J. Biol. Chem.
285
1208-1219
2010
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702419
Brockhausen
Site directed processing: role ...
Bos taurus, Homo sapiens
Biochim. Biophys. Acta
1790
1244-1257
2009
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704510
Perrine
Glycopeptide-preferring polype ...
Bos taurus, Homo sapiens
J. Biol. Chem.
284
20387-20397
2009
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704935
Gomes
Expression of UDP-N-acetyl-D-g ...
Homo sapiens
J. Histochem. Cytochem.
57
79-86
2009
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2
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706506
Guda
Inactivating germ-line and som ...
Homo sapiens
Proc. Natl. Acad. Sci. USA
106
12921-12925
2009
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1
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15
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1
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15
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2
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686299
Herr
Regulation of TGF-beta signall ...
Xenopus laevis
Development
135
1813-1822
2008
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1
1
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1
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686981
Gerken
Conservation of peptide accept ...
Bos taurus, Drosophila melanogaster, Homo sapiens
Glycobiology
18
861-870
2008
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1
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1
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4
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7
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-
-
-
-
-
16
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
2
-
4
-
-
-
-
-
-
-
-
16
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
687750
Raman
The catalytic and lectin domai ...
Homo sapiens
J. Biol. Chem.
283
22942-22951
2008
-
-
1
-
6
-
-
16
-
-
-
1
-
1
-
-
-
-
-
1
-
-
12
-
-
-
-
27
-
-
-
-
-
-
-
-
-
1
-
-
6
-
-
-
-
16
-
-
-
1
-
-
-
-
-
1
-
-
12
-
-
-
-
27
-
-
-
-
-
-
-
-
-
-
684219
Brooks
Immunolocalisation of members ...
Homo sapiens
Acta Histochem.
109
273-284
2007
-
-
-
-
-
-
-
-
2
-
-
5
-
5
-
-
-
-
-
6
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
10
-
-
5
-
-
-
-
-
27
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
686681
Tenno
Function of conserved aromatic ...
Rattus norvegicus
FEBS J.
274
6037-6045
2007
-
1
1
-
14
-
-
15
-
-
-
1
-
1
-
-
1
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
14
-
-
-
-
15
-
-
-
1
-
-
-
1
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
686971
Wandall
The lectin domains of polypept ...
Homo sapiens
Glycobiology
17
374-387
2007
-
1
1
-
7
5
13
22
1
-
-
4
-
4
-
-
1
-
-
-
23
-
36
-
-
-
-
-
-
-
-
-
-
-
32
-
4
4
-
-
7
5
34
23
-
22
4
-
-
4
-
-
-
4
-
-
25
-
36
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
687669
Tian
A UDP-GalNAc:polypeptide N-ace ...
Drosophila melanogaster
J. Biol. Chem.
282
606-614
2007
-
-
1
-
4
-
-
-
-
-
-
1
-
4
-
-
-
-
-
4
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
4
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
688380
Milac
Substrate-induced conformation ...
Homo sapiens
J. Mol. Biol.
373
439-451
2007
-
-
-
1
-
-
-
-
-
1
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
688968
Tenno
Initiation of protein O glycos ...
Mus musculus
Mol. Cell. Biol.
27
8783-8796
2007
-
-
1
-
3
-
-
-
-
-
-
1
-
4
-
-
-
-
-
5
-
-
4
-
-
-
-
-
-
-
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-
-
-
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1
-
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3
-
-
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1
-
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-
5
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
689866
Wu
Prokaryotic expression, purifi ...
Homo sapiens
Protein Expr. Purif.
56
1-7
2007
-
1
1
-
-
-
-
-
1
-
2
1
-
4
-
-
1
-
-
4
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
1
-
2
1
-
-
-
1
-
4
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
674772
Fritz
Dynamic association between th ...
Homo sapiens
J. Biol. Chem.
281
8613-8619
2006
-
-
-
1
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
658240
Nakamura
Cloning and expression of a br ...
Homo sapiens, Rattus norvegicus
Biol. Pharm. Bull.
28
429-433
2005
-
-
2
-
-
-
-
-
-
-
-
2
-
5
-
-
-
-
-
13
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
13
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
657665
Stwora-Wojczyk
Functional characterization of ...
Mus musculus, Rattus norvegicus, Toxoplasma gondii
Arch. Biochem. Biophys.
426
231-240
2004
-
-
1
-
-
-
-
9
-
-
-
-
-
6
-
-
1
-
-
-
-
-
14
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
9
-
-
-
-
-
-
-
1
-
-
-
-
14
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
657844
Freire
Characterization of a UDP-N-ac ...
Echinococcus granulosus
Biochem. J.
382
501-510
2004
-
-
1
-
-
-
-
-
-
7
-
1
-
4
-
-
-
-
-
3
-
-
3
-
1
1
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
7
-
1
-
-
-
-
-
3
-
-
3
-
1
1
-
-
1
1
-
-
-
-
-
-
-
-
658238
Nakamura
Characterization of a novel po ...
Drosophila sp. (in: Insecta)
Biol. Pharm. Bull.
27
1509-1514
2004
-
-
1
-
-
-
1
1
-
3
-
-
-
2
-
-
1
-
-
1
-
-
23
-
1
1
-
-
1
1
-
-
1
-
-
-
-
1
-
-
-
-
-
1
1
1
-
3
-
-
-
-
-
1
-
1
-
-
23
-
1
1
-
-
1
1
-
-
-
-
-
-
-
-
658739
Cheng
Characterization of a novel hu ...
Homo sapiens
FEBS Lett.
566
17-24
2004
-
-
1
-
-
-
-
4
-
-
-
-
-
3
-
-
-
-
-
18
-
-
10
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
18
-
-
10
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
658939
Stwora-Wojczyk
O-glycosylation in Toxoplasma ...
Toxoplasma gondii
Int. J. Parasitol.
34
309-322
2004
-
-
1
-
-
-
-
-
-
-
-
-
-
9
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
659929
Sangadala
Cloning, expression and proper ...
Sus scrofa
Mol. Cell. Biochem.
266
117-126
2004
-
-
1
-
-
-
-
-
-
-
-
-
-
4
-
-
1
-
-
1
1
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
1
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
660363
Fritz
The beginnings of mucin biosyn ...
Mus musculus
Proc. Natl. Acad. Sci. USA
101
15307-15312
2004
-
-
1
1
-
-
-
-
-
1
-
1
-
2
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
660415
Duclos
Characterization of the UDP-N- ...
Bos taurus
Protein Eng. Des. Sel.
17
635-646
2004
-
-
1
-
8
-
-
10
-
1
-
-
-
4
-
-
1
-
-
-
-
-
1
-
-
-
-
10
-
-
-
-
-
-
-
-
-
1
-
-
8
-
-
-
-
10
-
1
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
10
-
-
-
-
-
-
-
-
-
-
489183
Wang
Cloning and characterization o ...
Homo sapiens
Biochem. Biophys. Res. Commun.
300
738-744
2003
-
-
1
-
-
-
-
-
2
-
-
2
-
2
-
-
1
-
-
2
-
-
7
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
2
-
-
-
1
-
2
-
-
7
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
489184
Zhang
Cloning and characterization o ...
Homo sapiens, Mus musculus
J. Biol. Chem.
278
573-584
2003
-
-
2
-
1
-
-
12
2
-
-
4
-
5
-
-
1
-
-
7
-
-
7
-
2
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
1
-
-
-
-
12
2
-
-
4
-
-
-
1
-
9
-
-
7
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
489188
Iwasaki
Initiation of O-glycan synthes ...
Homo sapiens
J. Biol. Chem.
278
5613-5621
2003
-
-
1
-
-
-
-
-
-
-
-
2
-
2
-
-
1
-
-
2
-
-
6
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
1
-
2
-
-
6
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
489194
Wojczyk
cDNA cloning and expression of ...
Toxoplasma gondii
Mol. Biochem. Parasitol.
131
93-107
2003
-
-
1
-
-
-
-
-
2
-
-
-
-
4
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
636809
Marcos
Polypeptide GalNAc-transferase ...
Homo sapiens
J. Histochem. Cytochem.
51
761-771
2003
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
659250
Ten Hagen
Functional characterization an ...
Drosophila melanogaster
J. Biol. Chem.
278
35039-35048
2003
-
-
-
-
-
-
-
-
-
-
-
-
-
10
-
-
-
-
-
-
-
-
46
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
46
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
659778
Casaravilla
Mucin-type O-glycosylation in ...
Echinococcus granulosus, Fasciola hepatica, Toxocara canis
J. Parasitol.
89
709-714
2003
-
-
-
-
-
-
-
-
-
-
-
-
-
10
-
-
-
-
-
-
-
-
9
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
9
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
489190
Tenno
The lectin domain of UDP-GalNA ...
Rattus norvegicus
J. Biol. Chem.
277
47088-47096
2002
-
-
1
-
4
-
2
9
1
1
-
2
-
1
-
-
1
-
-
-
-
-
8
-
1
-
-
-
-
-
-
-
2
-
-
-
-
1
-
-
4
-
-
2
2
9
1
1
-
2
-
-
-
1
-
-
-
-
8
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
489191
Ten Hagen
A UDP-GalNAc:polypeptide N-ace ...
Drosophila melanogaster
J. Biol. Chem.
277
22616-22622
2002
-
-
1
-
3
-
-
2
1
-
1
1
-
3
-
-
1
-
-
2
-
-
5
1
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
3
-
-
-
-
2
1
-
1
1
-
-
-
1
-
2
-
-
5
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
489193
Takeuchi
O-GalNAc incorporation into a ...
Homo sapiens
Eur. J. Biochem.
269
6173-6183
2002
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
5
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
5
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
658686
Guo
-
Molecular cloning; characteriz ...
Homo sapiens
FEBS Lett.
26297
1-8
2002
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
659177
Sschwientek
Functional conservation of sub ...
Homo sapiens
J. Biol. Chem.
277
22623-22638
2002
-
-
-
-
-
-
-
-
-
-
-
-
-
7
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
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-
-
-
-
-
-
489182
Ten Hagen
Cloning and characterization o ...
Mus musculus, Rattus norvegicus
J. Biol. Chem.
276
17395-17404
2001
-
-
1
-
-
-
-
-
2
-
-
3
-
4
-
-
-
-
-
24
-
-
9
-
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-
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-
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24
-
-
9
-
2
-
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-
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-
-
-
-
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489187
Hanisch
Evidence for glycosylation-dep ...
Homo sapiens
Glycobiology
11
731-740
2001
-
-
1
-
-
-
2
-
1
-
-
1
-
1
-
-
1
-
-
-
-
-
6
-
1
-
-
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1
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1
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1
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6
-
1
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-
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-
-
-
-
-
489195
Katao
Distinct orders of GalNAc inco ...
Homo sapiens
Biochem. Biophys. Res. Commun.
287
110-115
2001
-
-
-
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-
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-
-
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2
-
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3
-
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-
-
-
-
-
-
489181
Toba
Brain-specific expression of a ...
Homo sapiens
Biochim. Biophys. Acta
1493
264-268
2000
-
-
1
-
-
-
-
-
1
-
-
2
-
2
-
-
-
-
-
2
-
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6
-
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1
-
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2
-
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6
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
489196
Iida
Order and maximum incorporatio ...
Homo sapiens
Biochem. J.
347
535-542
2000
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
1
-
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1
-
-
-
-
-
-
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1
-
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3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
489180
Bennett
A novel human UDP-N-acetyl-D-g ...
Homo sapiens
FEBS Lett.
460
226-230
1999
1
-
1
-
-
-
-
-
1
-
-
2
-
3
-
-
-
-
-
-
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
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-
1
-
-
2
-
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-
-
-
-
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
489185
Ten Hagen
Characterization of a UDP-GalN ...
Mus musculus, Rattus norvegicus
J. Biol. Chem.
274
27867-27874
1999
-
-
2
-
-
-
-
-
2
-
-
4
-
4
-
-
-
-
-
10
-
-
10
-
2
-
-
-
-
-
-
-
-
-
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-
-
2
-
-
-
-
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-
-
2
-
-
4
-
-
-
-
-
10
-
-
10
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
489189
Hagen
Structure-function analysis of ...
Mus musculus
J. Biol. Chem.
274
6797-6803
1999
-
-
1
-
24
-
-
-
2
2
-
1
-
2
-
-
1
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
24
-
-
-
-
-
2
2
-
1
-
-
-
1
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
659138
Bennett
Cloning and characterization o ...
Homo sapiens
J. Biol. Chem.
274
25362-25370
1999
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
489177
Mendicino
Purification and characterizat ...
Sus scrofa
Mol. Cell. Biochem.
185
135-145
1998
-
-
-
-
-
-
4
4
2
1
1
1
-
4
-
-
1
-
-
7
1
1
8
1
1
-
-
-
2
-
-
-
2
-
-
-
-
-
-
-
-
-
-
4
2
4
2
1
1
1
-
-
-
1
-
7
1
1
8
1
1
-
-
-
2
-
-
-
-
-
-
-
-
-
489192
Hennebicq
Polypeptide:N-acetylgalactosam ...
Homo sapiens
Biochimie
80
69-73
1998
-
1
-
-
-
-
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1
1
-
-
-
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2
-
-
-
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-
5
3
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2
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1
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-
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-
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1
-
-
-
-
-
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1
1
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-
-
-
-
-
-
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5
3
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
489197
Hagen
cDNA cloning and expression of ...
Caenorhabditis elegans
J. Biol. Chem.
273
8268-8277
1998
-
-
1
-
-
-
-
-
-
-
-
1
-
4
-
-
-
-
-
-
-
-
1
-
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-
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-
-
-
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-
-
-
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1
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-
-
-
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1
-
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-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
659135
Ten hagen
Cloning and expression of a no ...
Rattus norvegicus
J. Biol. Chem.
273
27749-27754
1998
-
-
1
-
-
-
-
4
-
-
-
-
-
3
-
-
-
-
-
3
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
3
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
659136
Bennett
Cloning of human UDP-N-acetyl- ...
Homo sapiens
J. Biol. Chem.
273
30472-30481
1998
-
-
1
-
1
-
-
12
-
-
-
-
-
5
-
-
1
-
-
29
-
-
30
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
1
-
-
-
-
12
-
-
-
-
-
-
-
1
-
31
-
-
30
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
489179
Wandall
Substrate specificities of thr ...
Homo sapiens
J. Biol. Chem.
272
23503-23514
1997
-
-
1
-
-
-
-
5
1
2
-
2
-
2
-
-
1
-
-
-
2
-
8
-
1
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
5
1
2
-
2
-
-
-
1
-
-
2
-
8
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
489198
Wragg
-
Identification of essential hi ...
Bos taurus
Biochem. J.
328
193-197
1997
-
-
1
-
4
-
1
-
-
-
-
1
-
1
-
1
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
4
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
489199
Hagen
cDNA cloning and expression of ...
Mus musculus
J. Biol. Chem.
272
13843-13848
1997
-
-
1
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
15
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
15
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
489176
Brockhausen
Specificity of O-glycosylation ...
Bos taurus
Glycoconj. J.
13
849-856
1996
-
-
-
-
-
-
-
3
-
-
-
2
-
2
-
-
1
-
-
1
1
-
4
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
2
-
-
-
1
-
1
1
-
4
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
657716
Zara
Cloning and expression of mous ...
Mus musculus
Biochem. Biophys. Res. Commun.
228
38-44
1996
-
-
1
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
659128
Bennett
cDNA cloning and expression of ...
Homo sapiens
J. Biol. Chem.
271
17006-17012
1996
-
-
1
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
489175
Homa
Conversion of a bovine UDP-Gal ...
Bos taurus
Protein Expr. Purif.
6
141-148
1995
-
-
1
-
-
-
-
3
7
-
1
1
-
4
-
1
1
-
-
-
1
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
3
7
-
1
1
-
-
1
1
-
-
1
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
489178
Soerensen
UDP-N-acetyl-alpha-D-galactosa ...
Homo sapiens, Ovis sp., Rattus norvegicus, Sus scrofa
J. Biol. Chem.
270
24166-24173
1995
-
-
-
-
-
-
2
5
-
-
2
3
-
6
-
-
3
-
-
13
2
-
25
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
5
-
-
2
3
-
-
-
3
-
13
2
-
25
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
489200
Wragg
Kinetic analysis of a recombin ...
Bos taurus
J. Biol. Chem.
270
16947-16954
1995
-
-
1
-
-
-
2
-
-
-
-
-
-
1
-
-
1
1
-
-
-
-
4
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
1
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
658856
Yoshida
Cloning and expression of a po ...
Sus scrofa
Glycoconj. J.
12
824-828
1995
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
658857
Hagen
Cloning and sequence homology ...
Rattus norvegicus
Glycoconj. J.
12
901-909
1995
-
-
1
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
10
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
10
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
659127
White
Purification and cDNA cloning ...
Homo sapiens
J. Biol. Chem.
270
24156-24165
1995
-
1
1
-
-
-
-
-
1
-
3
-
-
4
-
-
1
-
-
9
1
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
-
-
-
-
-
1
-
3
-
-
-
-
2
-
10
1
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
489201
Roth
Subcellular localization of th ...
Bos taurus, Sus scrofa
Proc. Natl. Acad. Sci. USA
91
8935-8939
1994
-
-
-
-
-
-
-
-
4
-
-
2
-
5
-
-
2
-
-
2
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
2
-
-
-
2
-
2
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
489202
Nishimaori
N-Acetylgalactosamine glycosyl ...
Homo sapiens
Cancer Res.
54
3738-3744
1994
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
2
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
489169
Wang
The acceptor substrate specifi ...
Sus scrofa
J. Biol. Chem.
268
22979-22983
1993
-
-
-
-
-
-
-
9
-
-
-
1
-
3
-
-
1
-
-
1
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
9
-
-
-
1
-
-
-
1
-
1
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
489186
Elhammer
The specificity of UDP-GalNAc: ...
Bos taurus
J. Biol. Chem.
268
10029-10038
1993
-
-
-
-
-
-
-
2
-
-
-
1
-
2
-
-
1
-
-
-
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
1
-
-
-
1
-
-
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
659124
Homa
Isolation and expression of a ...
Bos taurus
J. Biol. Chem.
268
12609-12616
1993
-
-
1
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3
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659126
Hagen
Purification, cloning, and exp ...
Bos taurus
J. Biol. Chem.
268
18960-18965
1993
-
-
1
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489170
Wang
Purification and characterizat ...
Sus scrofa
J. Biol. Chem.
267
12709-12716
1992
1
-
-
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1
4
1
6
1
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3
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4
1
6
1
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1
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2
2
2
7
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1
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1
1
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489174
Cottrell
Polypeptide N-acetylgalactosam ...
Sus scrofa
Biochem. J.
283
299-305
1992
1
-
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2
1
1
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4
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489171
Hatanaka
Synthesis of a new inhibitor o ...
Sus scrofa
Biochem. Biophys. Res. Commun.
175
668-672
1991
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3
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489172
Elhammer
Purification and characterizat ...
Bos taurus, Mus musculus
J. Biol. Chem.
261
5249-5255
1986
-
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3
3
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4
2
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4
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1
10
2
2
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489168
Takeuchi
-
Purification and characterizat ...
Bos taurus
Agric. Biol. Chem.
49
1059-1069
1985
2
1
-
-
-
-
16
2
1
5
1
1
-
1
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6
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1
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16
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1
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6
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-
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-
489167
Sugiura
Purification and characterizat ...
Rattus norvegicus
J. Biol. Chem.
257
9501-9507
1982
-
-
-
-
-
-
5
1
1
3
2
-
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1
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1
2
1
9
1
1
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1
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5
3
1
1
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1
2
1
9
1
1
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1
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-
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489173
Briand
Investigation of the requireme ...
Bos taurus
J. Biol. Chem.
256
12205-12207
1981
-
-
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-
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-
6
1
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1
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1
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6
1
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1
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1
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