Literature summary for 2.4.1.41 extracted from

Soerensen, T.; White, T.; Wandall, H.H.; Kristensen, A.K.; Roepstorff, P.; Clausen, H.
UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase. Identification and separation of two distinct transferase activities (1995), J. Biol. Chem., 270, 24166-24173.

Inhibitors

Inhibitors	Comment	Organism	Structure
HIV-V3	competitive inhibition of Muc2 glycosylation	Homo sapiens
Muc2	competitive inhibition of HIV-V3 peptide glycosylation	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism
additional information	-	additional information	-	Rattus norvegicus
additional information	-	additional information	-	Sus scrofa
additional information	-	additional information	values for different human mucin Muc1 peptides	Homo sapiens
0.23	-	human mucin Muc2 peptide	-	Homo sapiens
0.5	-	HIV-V3 peptide	-	Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
100000	-	gel filtration	Sus scrofa
100000	-	gel filtration	Ovis sp.

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.
UDP-N-acetyl-D-galactosamine + polypeptide	Sus scrofa	-	UDP + N-acetyl-D-galactosaminyl-polypeptide	-	?
UDP-N-acetyl-D-galactosamine + polypeptide	Homo sapiens	different enzyme activities are involved in the initiation of GalNAc O-glycosylation and these are differentially expressed in cells and organs	UDP + N-acetyl-D-galactosaminyl-polypeptide	-	?
UDP-N-acetyl-D-galactosamine + polypeptide	Rattus norvegicus	different enzyme activities are involved in the initiation of GalNAc O-glycosylation and these are differentially expressed in cells and organs	UDP + N-acetyl-D-galactosaminyl-polypeptide	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-
Ovis sp.	-	-	-
Rattus norvegicus	-	male rats	-
Sus scrofa	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
placenta enzyme, Muc2 peptide affinity chromatography separates two distinct transferase activities with overlapping specificity concerning Muc2 substrate, but which are distinguishable with respect to HIV-V3 substrate	Homo sapiens
submaxillary gland enzyme, 123.5fold	Ovis sp.
submaxillary gland enzyme, 25.4fold	Sus scrofa

Source Tissue

Source Tissue	Comment	Organism	Textmining
heart ventricle	-	Rattus norvegicus	-
kidney	-	Homo sapiens	-
kidney	expresses GalNAc-T2	Rattus norvegicus	-
liver	expresses significantly more GalNAc-T2 than GalNAc-T1	Homo sapiens	-
additional information	quantitative and qualitative differences in different organs	Homo sapiens	-
additional information	quantitative and qualitative differences in different organs	Rattus norvegicus	-
placenta	-	Homo sapiens	-
salivary gland	-	Rattus norvegicus	-
salivary gland	-	Sus scrofa	-
salivary gland	-	Ovis sp.	-
submaxillary gland	-	Sus scrofa	-
submaxillary gland	-	Ovis sp.	-
testis	-	Rattus norvegicus	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.09	-	-	Sus scrofa
0.11	-	-	Ovis sp.

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
additional information	different substrate specificities before and after Muc2 affinity chromatography of enzyme	Homo sapiens	?	-	?
additional information	different substrate specificities before and after Muc2 affinity chromatography of enzyme	Sus scrofa	?	-	?
additional information	different substrate specificities before and after Muc2 affinity chromatography of enzyme	Ovis sp.	?	-	?
additional information	existence of multiple enzyme activities, differentially expressed in different organs, at least 2 distinct enzyme specificities	Homo sapiens	?	-	?
additional information	existence of multiple enzyme activities, differentially expressed in different organs, at least 2 distinct enzyme specificities	Rattus norvegicus	?	-	?
UDP-N-acetyl-alpha-D-galactosamine + human mucin Muc2 peptide	-	Homo sapiens	?	-	?
UDP-N-acetyl-D-galactosamine + HIV-V3 peptide	submaxillary gland enzyme, low activity	Sus scrofa	UDP + N-acetyl-D-galactosaminyl-HIV-V3 peptide	-	?
UDP-N-acetyl-D-galactosamine + HIV-V3 peptide	submaxillary gland enzyme, low activity	Ovis sp.	UDP + N-acetyl-D-galactosaminyl-HIV-V3 peptide	-	?
UDP-N-acetyl-D-galactosamine + HIV-V3 peptide	placenta, but not liver enzyme	Homo sapiens	UDP + N-acetyl-D-galactosaminyl-HIV-V3 peptide	-	?
UDP-N-acetyl-D-galactosamine + human chorionic gonadotropin-beta peptide	placenta enzyme	Homo sapiens	UDP + N-acetyl-D-galactosaminyl-human chorionic gonadotropin-beta peptide	-	?
UDP-N-acetyl-D-galactosamine + human chorionic gonadotropin-beta peptide	only Ser acceptor sites, with a very low activity	Homo sapiens	UDP + N-acetyl-D-galactosaminyl-human chorionic gonadotropin-beta peptide	-	?
UDP-N-acetyl-D-galactosamine + human chorionic gonadotropin-beta peptide	only Ser acceptor sites, with a very low activity	Sus scrofa	UDP + N-acetyl-D-galactosaminyl-human chorionic gonadotropin-beta peptide	-	?
UDP-N-acetyl-D-galactosamine + human chorionic gonadotropin-beta peptide	only Ser acceptor sites, with a very low activity	Ovis sp.	UDP + N-acetyl-D-galactosaminyl-human chorionic gonadotropin-beta peptide	-	?
UDP-N-acetyl-D-galactosamine + human chorionic gonadotropin-beta peptide	submaxillary gland enzyme	Sus scrofa	UDP + N-acetyl-D-galactosaminyl-human chorionic gonadotropin-beta peptide	-	?
UDP-N-acetyl-D-galactosamine + human chorionic gonadotropin-beta peptide	submaxillary gland enzyme	Ovis sp.	UDP + N-acetyl-D-galactosaminyl-human chorionic gonadotropin-beta peptide	-	?
UDP-N-acetyl-D-galactosamine + polypeptide	-	Sus scrofa	UDP + N-acetyl-D-galactosaminyl-polypeptide	-	?
UDP-N-acetyl-D-galactosamine + polypeptide	acceptor: SIV3 peptide	Sus scrofa	UDP + N-acetyl-D-galactosaminyl-polypeptide	-	?
UDP-N-acetyl-D-galactosamine + polypeptide	transfer of GalNAc to a serine or threonine residue on the acceptor protein	Homo sapiens	UDP + N-acetyl-D-galactosaminyl-polypeptide	-	?
UDP-N-acetyl-D-galactosamine + polypeptide	transfer of GalNAc to a serine or threonine residue on the acceptor protein	Rattus norvegicus	UDP + N-acetyl-D-galactosaminyl-polypeptide	-	?
UDP-N-acetyl-D-galactosamine + polypeptide	transfer of GalNAc to a serine or threonine residue on the acceptor protein	Sus scrofa	UDP + N-acetyl-D-galactosaminyl-polypeptide	-	?
UDP-N-acetyl-D-galactosamine + polypeptide	transfer of GalNAc to a serine or threonine residue on the acceptor protein	Ovis sp.	UDP + N-acetyl-D-galactosaminyl-polypeptide	-	?
UDP-N-acetyl-D-galactosamine + polypeptide	acceptors: human mucin Muc1 and Muc2 peptides, human chorionic gonadotropin-beta peptide, HIV-V3 peptides	Homo sapiens	UDP + N-acetyl-D-galactosaminyl-polypeptide	-	?
UDP-N-acetyl-D-galactosamine + polypeptide	acceptors: human mucin Muc1 and Muc2 peptides, human chorionic gonadotropin-beta peptide, HIV-V3 peptides	Sus scrofa	UDP + N-acetyl-D-galactosaminyl-polypeptide	-	?
UDP-N-acetyl-D-galactosamine + polypeptide	acceptors: human mucin Muc1 and Muc2 peptides, human chorionic gonadotropin-beta peptide, HIV-V3 peptides	Ovis sp.	UDP + N-acetyl-D-galactosaminyl-polypeptide	-	?
UDP-N-acetyl-D-galactosamine + polypeptide	different enzyme activities are involved in the initiation of GalNAc O-glycosylation and these are differentially expressed in cells and organs	Homo sapiens	UDP + N-acetyl-D-galactosaminyl-polypeptide	-	?
UDP-N-acetyl-D-galactosamine + polypeptide	different enzyme activities are involved in the initiation of GalNAc O-glycosylation and these are differentially expressed in cells and organs	Rattus norvegicus	UDP + N-acetyl-D-galactosaminyl-polypeptide	-	?

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Homo sapiens
37	-	assay at	Rattus norvegicus
37	-	assay at	Sus scrofa