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Literature summary for 2.4.1.40 extracted from

  • Blackler, R.J.; Gagnon, S.M.; Polakowski, R.; Rose, N.L.; Zheng, R.B.; Letts, J.A.; Johal, A.R.; Schuman, B.; Borisova, S.N.; Palcic, M.M.; Evans, S.V.
    Glycosyltransfer in mutants of putative catalytic residue Glu303 of the human ABO(H) A and B blood group glycosyltransferases GTA and GTB proceeds through a labile active site (2017), Glycobiology, 27, 370-380 .
    View publication on PubMed
Search for more literature for 2.4.1.40

Crystallization (Commentary)

Crystallization (Comment) Organism
wild-type and mutants E303A, E303C, E303D, E303Q Homo sapiens

Protein Variants

Protein Variants Comment Organism
E303A residue E303 plays a critical role in maintaining the stability of a strained double-turn in the active site through several hydrogen bonds Homo sapiens
E303C residue E303 plays a critical role in maintaining the stability of a strained double-turn in the active site through several hydrogen bonds. Mutant retains significant activity despite disrupted active site architecture Homo sapiens
E303D residue E303 plays a critical role in maintaining the stability of a strained double-turn in the active site through several hydrogen bonds. Mutant retains significant activity despite disrupted active site architecture Homo sapiens
E303Q residue E303 plays a critical role in maintaining the stability of a strained double-turn in the active site through several hydrogen bonds Homo sapiens
additional information the enzymes GTA, EC 2.4.1.40, and GTB have nearly identical sequences, while the corresponding mutants of GTA/GTB have up to a 13fold difference in their residual activities relative to wild type.The mutated Cys, Asp and Gln functional groups are no more than 0.8 A further from the anomeric carbon of donor substrate compared to wild type Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0087
-
 UDP-N-acetyl-alpha-D-galactosamine wild-type, pH 7.0, 37°C Homo sapiens
0.03
-
 UDP-N-acetyl-alpha-D-galactosamine mutant E303A, pH 7.0, 37°C Homo sapiens
0.036
-
 alpha-Fuc-(1->2)-beta-D-Gal-(CH2)7CH3 mutant E303Q, pH 7.0, 37°C Homo sapiens
0.05
-
 UDP-N-acetyl-alpha-D-galactosamine mutant E303Q, pH 7.0, 37°C Homo sapiens
0.051
-
 UDP-N-acetyl-alpha-D-galactosamine mutant E303C, pH 7.0, 37°C Homo sapiens
0.086
-
 UDP-N-acetyl-alpha-D-galactosamine mutant E303D, pH 7.0, 37°C Homo sapiens
0.099
-
 alpha-Fuc-(1->2)-beta-D-Gal-(CH2)7CH3 wild-type, pH 7.0, 37°C Homo sapiens
0.27
-
 alpha-Fuc-(1->2)-beta-D-Gal-(CH2)7CH3 mutant E303D, pH 7.0, 37°C Homo sapiens
0.4
-
 alpha-Fuc-(1->2)-beta-D-Gal-(CH2)7CH3 mutant E303C, pH 7.0, 37°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P16442
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-N-acetyl-alpha-D-galactosamine + alpha-Fuc-(1->2)-beta-D-Gal-(CH2)7CH3
-
 Homo sapiens UDP + N-acetyl-alpha-D-galactosaminyl-(1->3)-[alpha-Fuc-(1->2)]-beta-D-Gal-(CH2)7CH3
-
 ?

Synonyms

Synonyms Comment Organism
GTA
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0047
-
 alpha-Fuc-(1->2)-beta-D-Gal-(CH2)7CH3 mutant E303A, pH 7.0, 37°C Homo sapiens
0.027
-
 alpha-Fuc-(1->2)-beta-D-Gal-(CH2)7CH3 mutant E303Q, pH 7.0, 37°C Homo sapiens
0.09
-
 alpha-Fuc-(1->2)-beta-D-Gal-(CH2)7CH3 mutant E303C, pH 7.0, 37°C Homo sapiens
2.2
-
 alpha-Fuc-(1->2)-beta-D-Gal-(CH2)7CH3 mutant E303D, pH 7.0, 37°C Homo sapiens
17.5
-
 alpha-Fuc-(1->2)-beta-D-Gal-(CH2)7CH3 wild-type, pH 7.0, 37°C Homo sapiens