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Literature summary for 2.4.1.4 extracted from
- Site-specific alpha-glycosylation of hydroxyflavones and hydroxyflavanones by amylosucrase from Deinococcus geothermalis (2019), Enzyme Microb. Technol., 129, 109361 .
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | transglycosylation reactions with amylosucrase from Deinococcus geothermalis constitute an efficient and economical method to produce alpha-glucosyl flavonoids | Deinococcus geothermalis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.31 | - |
6-hydroxyflavanone | pH 7.0, 45°C | Deinococcus geothermalis |  |
| 3.9 | - |
6-hydroxyflavanone | pH 5.0, 45°C | Deinococcus geothermalis | |
| 4.67 | - |
4'-hydroxyflavanone | pH 7.0, 45°C | Deinococcus geothermalis | |
| 9.07 | - |
4'-hydroxyflavanone | pH 5.0, 45°C | Deinococcus geothermalis | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Deinococcus geothermalis | Q1J0W0 | - |
- |
| Deinococcus geothermalis DSM 11300 | Q1J0W0 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sucrose + 4'-hydroxyflavanone | 3-OH and 7-OH positions of the mono-hydroxyflavones and -hydroxyflavanones are resistant to transglycosylation by Deinococcus geothermalis amylosucrase, whereas the 6-OH and 4'-OH positions of the mono-hydroxyflavones and mono-hydroxyflavanones exhibit relatively strong transglycosylation reactivities with the glucose donors released from sucrose by amylosucrase from Deinococcus geothermalis. The 6-OH position is considerably more reactive (54fold higher kcat) than the 4'-OH position in both hydroxyflavones and hydroxyflavanones. Further, the transglycosylation reactions with di- and tri-hydroxyflavones and hydroxyflavanones are also investigated and observed to exhibit similar results to those observed for the mono-hydroxyflavones and mono-hydroxyflavanones molecules | Deinococcus geothermalis | D-fructose + ? | - |
? | |
| sucrose + 4'-hydroxyflavanone | 3-OH and 7-OH positions of the mono-hydroxyflavones and -hydroxyflavanones are resistant to transglycosylation by Deinococcus geothermalis amylosucrase, whereas the 6-OH and 4'-OH positions of the mono-hydroxyflavones and mono-hydroxyflavanones exhibit relatively strong transglycosylation reactivities with the glucose donors released from sucrose by amylosucrase from Deinococcus geothermalis. The 6-OH position is considerably more reactive (54fold higher kcat) than the 4'-OH position in both hydroxyflavones and hydroxyflavanones. Further, the transglycosylation reactions with di- and tri-hydroxyflavones and hydroxyflavanones are also investigated and observed to exhibit similar results to those observed for the mono-hydroxyflavones and mono-hydroxyflavanones molecules | Deinococcus geothermalis DSM 11300 | D-fructose + ? | - |
? | |
| sucrose + 6-hydroxyflavanone | 3-OH and 7-OH positions of the mono-hydroxyflavones and -hydroxyflavanones are resistant to transglycosylation by Deinococcus geothermalis amylosucrase, whereas the 6-OH and 4'-OH positions of the mono-hydroxyflavones and -hydroxyflavanones exhibit relatively strong transglycosylation reactivities with the glucose donors released from sucrose by amylosucrase from Deinococcus geothermalis. The 6-OH position is considerably more reactive (54fold higher kcat) than the 4'-OH position in both hydroxyflavones and hydroxyflavanones. Further, the transglycosylation reactions with di- and tri-hydroxyflavones and hydroxyflavanones are also investigated and observed to exhibit similar results to those observed for the mono-hydroxyflavones and mono-hydroxyflavanones molecules | Deinococcus geothermalis | D-fructose + ? | - |
? | |
| sucrose + 6-hydroxyflavanone | 3-OH and 7-OH positions of the mono-hydroxyflavones and -hydroxyflavanones are resistant to transglycosylation by Deinococcus geothermalis amylosucrase, whereas the 6-OH and 4'-OH positions of the mono-hydroxyflavones and -hydroxyflavanones exhibit relatively strong transglycosylation reactivities with the glucose donors released from sucrose by amylosucrase from Deinococcus geothermalis. The 6-OH position is considerably more reactive (54fold higher kcat) than the 4'-OH position in both hydroxyflavones and hydroxyflavanones. Further, the transglycosylation reactions with di- and tri-hydroxyflavones and hydroxyflavanones are also investigated and observed to exhibit similar results to those observed for the mono-hydroxyflavones and mono-hydroxyflavanones molecules | Deinococcus geothermalis DSM 11300 | D-fructose + ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ASASE | - |
Deinococcus geothermalis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Deinococcus geothermalis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0038 | - |
4'-hydroxyflavanone | pH 5.0, 45°C | Deinococcus geothermalis | |
| 0.015 | - |
4'-hydroxyflavanone | pH 7.0, 45°C | Deinococcus geothermalis | |
| 0.71 | - |
6-hydroxyflavanone | pH 5.0, 45°C | Deinococcus geothermalis | |
| 0.84 | - |
6-hydroxyflavanone | pH 7.0, 45°C | Deinococcus geothermalis | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0005 | - |
4'-hydroxyflavanone | pH 5.0, 45°C | Deinococcus geothermalis | |
| 0.0032 | - |
4'-hydroxyflavanone | pH 7.0, 45°C | Deinococcus geothermalis | |
| 0.1835 | - |
6-hydroxyflavanone | pH 5.0, 45°C | Deinococcus geothermalis | |
| 0.6943 | - |
6-hydroxyflavanone | pH 7.0, 45°C | Deinococcus geothermalis | |
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