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Literature summary for 2.4.1.4 extracted from

  • Kim, M.; Seo, D.; Jung, J.; Jung, D.; Joe, M.; Lim, S.; Lee, J.; Park, C.
    Molecular cloning and expression of amylosucrase from highly radiation-resistant Deinococcus radiopugnans (2014), Food Sci. Biotechnol., 23, 2007-2012.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
gene drpas, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of the His-tagged enzyme in Escherichia coli Deinococcus radiopugnans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
72000
-
2 * 72000, recombinant His-tagged enzyme, SDS-PAGE, 2 * 72822, sequence calculation Deinococcus radiopugnans
72822
-
2 * 72000, recombinant His-tagged enzyme, SDS-PAGE, 2 * 72822, sequence calculation Deinococcus radiopugnans
152100
-
recombinant His-tagged enzyme, gel filtration Deinococcus radiopugnans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Deinococcus radiopugnans amylosucrase is a versatile enzyme that carries out 3 different catalytic reactions: 1. hydrolysis of sucrose to release a glucose molecule and a fructose molecule, 2. synthesis of glucose polymers from liberated glucose molecules, and 3. production of the sucrose isomers turanose and isomaltulose through an isomerization reaction. In addition, the enzyme can attach glucose molecules to an atypical substrate, thereby generating unnatural glucan-conjugates. The enzyme produces glucose, fructose, soluble maltooligosaccharide, insoluble glucan, and sucrose isomers (turanose and trehalulose) using only sucrose as a substrate ?
-
?
additional information Deinococcus radiopugnans ATCC 19172 amylosucrase is a versatile enzyme that carries out 3 different catalytic reactions: 1. hydrolysis of sucrose to release a glucose molecule and a fructose molecule, 2. synthesis of glucose polymers from liberated glucose molecules, and 3. production of the sucrose isomers turanose and isomaltulose through an isomerization reaction. In addition, the enzyme can attach glucose molecules to an atypical substrate, thereby generating unnatural glucan-conjugates. The enzyme produces glucose, fructose, soluble maltooligosaccharide, insoluble glucan, and sucrose isomers (turanose and trehalulose) using only sucrose as a substrate ?
-
?

Organism

Organism UniProt Comment Textmining
Deinococcus radiopugnans
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gene drpas
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Deinococcus radiopugnans ATCC 19172
-
gene drpas
-

Purification (Commentary)

Purification (Comment) Organism
recombinant the His-tagged enzyme from Escherichia coli by nickel affinity chromatography Deinococcus radiopugnans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information amylosucrase is a versatile enzyme that carries out 3 different catalytic reactions: 1. hydrolysis of sucrose to release a glucose molecule and a fructose molecule, 2. synthesis of glucose polymers from liberated glucose molecules, and 3. production of the sucrose isomers turanose and isomaltulose through an isomerization reaction. In addition, the enzyme can attach glucose molecules to an atypical substrate, thereby generating unnatural glucan-conjugates. The enzyme produces glucose, fructose, soluble maltooligosaccharide, insoluble glucan, and sucrose isomers (turanose and trehalulose) using only sucrose as a substrate Deinococcus radiopugnans ?
-
?
additional information the enzyme does not require a nucleotide-activated sugar as a glucosyl-donor Deinococcus radiopugnans ?
-
?
additional information amylosucrase is a versatile enzyme that carries out 3 different catalytic reactions: 1. hydrolysis of sucrose to release a glucose molecule and a fructose molecule, 2. synthesis of glucose polymers from liberated glucose molecules, and 3. production of the sucrose isomers turanose and isomaltulose through an isomerization reaction. In addition, the enzyme can attach glucose molecules to an atypical substrate, thereby generating unnatural glucan-conjugates. The enzyme produces glucose, fructose, soluble maltooligosaccharide, insoluble glucan, and sucrose isomers (turanose and trehalulose) using only sucrose as a substrate Deinococcus radiopugnans ATCC 19172 ?
-
?
additional information the enzyme does not require a nucleotide-activated sugar as a glucosyl-donor Deinococcus radiopugnans ATCC 19172 ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 72000, recombinant His-tagged enzyme, SDS-PAGE, 2 * 72822, sequence calculation Deinococcus radiopugnans

Synonyms

Synonyms Comment Organism
DRpAS
-
Deinococcus radiopugnans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40 45 recombinant His-tagged enzyme Deinococcus radiopugnans

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 50 activity range, profile overview Deinococcus radiopugnans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40 50 half-life of the enzyme is 421.4 h at 40°C and 3.3 h at 45°C, the thermostability of DRpAS dramatically decreases at 50°C, Tm value is 50.7°C and the half-life of the enzyme is 7.2 min at 50°C Deinococcus radiopugnans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
recombinant His-tagged enzyme Deinococcus radiopugnans

pI Value

Organism Comment pI Value Maximum pI Value
Deinococcus radiopugnans sequence calculation
-
5.3

General Information

General Information Comment Organism
evolution the enzyme is a glucosyltransferase of the glycoside hydrolase 13 family, that does not require a nucleotide-activated sugar as a glucosyl-donor Deinococcus radiopugnans
additional information loops 3, 4, and 7 of AS form an active-site pocket and play an important role in transglycosylation activity Deinococcus radiopugnans