Cloned (Comment) | Organism |
---|---|
gene drpas, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of the His-tagged enzyme in Escherichia coli | Deinococcus radiopugnans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
72000 | - |
2 * 72000, recombinant His-tagged enzyme, SDS-PAGE, 2 * 72822, sequence calculation | Deinococcus radiopugnans |
72822 | - |
2 * 72000, recombinant His-tagged enzyme, SDS-PAGE, 2 * 72822, sequence calculation | Deinococcus radiopugnans |
152100 | - |
recombinant His-tagged enzyme, gel filtration | Deinococcus radiopugnans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Deinococcus radiopugnans | amylosucrase is a versatile enzyme that carries out 3 different catalytic reactions: 1. hydrolysis of sucrose to release a glucose molecule and a fructose molecule, 2. synthesis of glucose polymers from liberated glucose molecules, and 3. production of the sucrose isomers turanose and isomaltulose through an isomerization reaction. In addition, the enzyme can attach glucose molecules to an atypical substrate, thereby generating unnatural glucan-conjugates. The enzyme produces glucose, fructose, soluble maltooligosaccharide, insoluble glucan, and sucrose isomers (turanose and trehalulose) using only sucrose as a substrate | ? | - |
? | |
additional information | Deinococcus radiopugnans ATCC 19172 | amylosucrase is a versatile enzyme that carries out 3 different catalytic reactions: 1. hydrolysis of sucrose to release a glucose molecule and a fructose molecule, 2. synthesis of glucose polymers from liberated glucose molecules, and 3. production of the sucrose isomers turanose and isomaltulose through an isomerization reaction. In addition, the enzyme can attach glucose molecules to an atypical substrate, thereby generating unnatural glucan-conjugates. The enzyme produces glucose, fructose, soluble maltooligosaccharide, insoluble glucan, and sucrose isomers (turanose and trehalulose) using only sucrose as a substrate | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Deinococcus radiopugnans | - |
gene drpas | - |
Deinococcus radiopugnans ATCC 19172 | - |
gene drpas | - |
Purification (Comment) | Organism |
---|---|
recombinant the His-tagged enzyme from Escherichia coli by nickel affinity chromatography | Deinococcus radiopugnans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | amylosucrase is a versatile enzyme that carries out 3 different catalytic reactions: 1. hydrolysis of sucrose to release a glucose molecule and a fructose molecule, 2. synthesis of glucose polymers from liberated glucose molecules, and 3. production of the sucrose isomers turanose and isomaltulose through an isomerization reaction. In addition, the enzyme can attach glucose molecules to an atypical substrate, thereby generating unnatural glucan-conjugates. The enzyme produces glucose, fructose, soluble maltooligosaccharide, insoluble glucan, and sucrose isomers (turanose and trehalulose) using only sucrose as a substrate | Deinococcus radiopugnans | ? | - |
? | |
additional information | the enzyme does not require a nucleotide-activated sugar as a glucosyl-donor | Deinococcus radiopugnans | ? | - |
? | |
additional information | amylosucrase is a versatile enzyme that carries out 3 different catalytic reactions: 1. hydrolysis of sucrose to release a glucose molecule and a fructose molecule, 2. synthesis of glucose polymers from liberated glucose molecules, and 3. production of the sucrose isomers turanose and isomaltulose through an isomerization reaction. In addition, the enzyme can attach glucose molecules to an atypical substrate, thereby generating unnatural glucan-conjugates. The enzyme produces glucose, fructose, soluble maltooligosaccharide, insoluble glucan, and sucrose isomers (turanose and trehalulose) using only sucrose as a substrate | Deinococcus radiopugnans ATCC 19172 | ? | - |
? | |
additional information | the enzyme does not require a nucleotide-activated sugar as a glucosyl-donor | Deinococcus radiopugnans ATCC 19172 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 72000, recombinant His-tagged enzyme, SDS-PAGE, 2 * 72822, sequence calculation | Deinococcus radiopugnans |
Synonyms | Comment | Organism |
---|---|---|
DRpAS | - |
Deinococcus radiopugnans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 45 | recombinant His-tagged enzyme | Deinococcus radiopugnans |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 50 | activity range, profile overview | Deinococcus radiopugnans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 50 | half-life of the enzyme is 421.4 h at 40°C and 3.3 h at 45°C, the thermostability of DRpAS dramatically decreases at 50°C, Tm value is 50.7°C and the half-life of the enzyme is 7.2 min at 50°C | Deinococcus radiopugnans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
recombinant His-tagged enzyme | Deinococcus radiopugnans |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Deinococcus radiopugnans | sequence calculation | - |
5.3 |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme is a glucosyltransferase of the glycoside hydrolase 13 family, that does not require a nucleotide-activated sugar as a glucosyl-donor | Deinococcus radiopugnans |
additional information | loops 3, 4, and 7 of AS form an active-site pocket and play an important role in transglycosylation activity | Deinococcus radiopugnans |