Cloned (Comment) | Organism |
---|---|
gene dgas, recombinant expression of C-terminally His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Deinococcus geothermalis |
gene npas, recombinant expression of C-terminally His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Neisseria polysaccharea |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of chimeric enzymes using gene dgas and gene npas from Deinococcus geothermalis by overlap extension polymerase chain reaction, the mutants show altered polymerization activity and thermostability | Neisseria polysaccharea |
additional information | construction of chimeric enzymes using gene dgas and gene npas from Neisseria polysaccharea by overlap extension polymerase chain reaction, the mutants show altered polymerization activity and thermostability. Three-dimensional modeling structure and molecular dynamics of mutant DGAS-B, quaternary structure | Deinococcus geothermalis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Neisseria polysaccharea | amylosucrase synthesizes alpha-1,4-glucans using sucrose as a sole substrate | ? | - |
? | |
additional information | Deinococcus geothermalis | amylosucrase synthesizes alpha-1,4-glucans using sucrose as a sole substrate | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Deinococcus geothermalis | Q1J0W0 | gene dgas | - |
Neisseria polysaccharea | Q9ZEU2 | gene npas | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Neisseria polysaccharea |
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Deinococcus geothermalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | amylosucrase synthesizes alpha-1,4-glucans using sucrose as a sole substrate | Neisseria polysaccharea | ? | - |
? | |
additional information | amylosucrase synthesizes alpha-1,4-glucans using sucrose as a sole substrate | Deinococcus geothermalis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the active pocket in the enzyme is surrounded by A, B, and B' domains. The A domain serves as a catalytic domain and has the typical (beta/alpha)8-barrel structure of the GH13 family. The B' domain includes oligosaccharide binding sites, and both the B and B' domains of the enzyme contain acceptor subsites (+2, +3, +4, +5, and +6) | Deinococcus geothermalis |
More | the active pocket in the enzyme is surrounded by A, B, and B' domains. The A domain serves as a catalytic domain andhas the typical (beta/alpha)8-barrel structure of the GH13 family. The B' domain includes oligosaccharide binding sites, and both the B and B' domains of the enzyme contain acceptor subsites (+2, +3, +4, +5, and +6) | Neisseria polysaccharea |
Synonyms | Comment | Organism |
---|---|---|
ASASE | - |
Neisseria polysaccharea |
ASASE | - |
Deinococcus geothermalis |
DGAS | - |
Deinococcus geothermalis |
NPAS | - |
Neisseria polysaccharea |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 55 | recombinant chimeric enzymes | Neisseria polysaccharea |
30 | 55 | recombinant chimeric enzymes | Deinococcus geothermalis |
40 | - |
assay at, wild-type enzyme | Deinococcus geothermalis |
45 | - |
assay at, wild-type enzyme | Neisseria polysaccharea |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
43.2 | 51.5 | the half-lives of mutant NPAS-B' and wild-type NPAS are 4.28 and 9.99 min at 45°C and their melting temperatures are 43.25°C and 51.52°C, respectively | Neisseria polysaccharea |
50 | 62 | half-lives of mutant DGAS-B are 30.70 and 3.39 min at 50°C and 55°C, respectively, whereas that of the wild-type DGAS is 65.74 min at 55°C. The melting temperatures of mutant DGAS-B and wild-type DGAS are 54.23°C and 61.42°C, respectively | Deinococcus geothermalis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | wild-type enzyme | Deinococcus geothermalis |
7 | - |
recombinant chimeric enzyme DGAS-B | Deinococcus geothermalis |
8 | - |
recombinant chimeric enzyme NPAS-B' | Neisseria polysaccharea |
8 | 9 | wild-type enzyme | Neisseria polysaccharea |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 9.5 | activity range, wild-type and mutant enzymes, profile overview | Neisseria polysaccharea |
5.5 | 9.5 | activity range, wild-type and mutant enzymes, profile overview | Deinococcus geothermalis |