Cloned (Comment) | Organism |
---|---|
expression of GST-tagged ASase in Escherichia coli strain BL21 (DE3) | Deinococcus geothermalis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
73000 | - |
x * 73214, sequence calculation, x * 73000, recombinant detagged enzyme, SDS-PAGE | Deinococcus geothermalis |
73214 | - |
x * 73214, sequence calculation, x * 73000, recombinant detagged enzyme, SDS-PAGE | Deinococcus geothermalis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose + [(1->4)-alpha-D-glucosyl]n | Deinococcus geothermalis | - |
D-fructose + [(1->4)-alpha-D-glucosyl]n+1 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Deinococcus geothermalis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant GST-tagged ASase from Escherichia coli strain BL21 (DE3) by glutathione affinity chromatography, the tag is cleaved off by thrombin | Deinococcus geothermalis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.8 | - |
sucrose hydrolysis, purified recombinant GST-tagged DgAS, pH 7.0, 37°C | Deinococcus geothermalis |
5 | - |
sucrose hydrolysis, purified recombinant detagged DgAS, pH 7.0, 37°C | Deinococcus geothermalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | enzymatic production of trehalose from sucrose using amylosucrase and maltooligosyltrehalose synthase-trehalohydrolase, overview | Deinococcus geothermalis | ? | - |
? | |
sucrose + [(1->4)-alpha-D-glucosyl]n | - |
Deinococcus geothermalis | D-fructose + [(1->4)-alpha-D-glucosyl]n+1 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 73214, sequence calculation, x * 73000, recombinant detagged enzyme, SDS-PAGE | Deinococcus geothermalis |
Synonyms | Comment | Organism |
---|---|---|
ASASE | - |
Deinococcus geothermalis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Deinococcus geothermalis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Deinococcus geothermalis |