Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.1.4 extracted from

  • Kim, H.; Jung, J.; Seo, D.; Ha, S.; Yoo, S.; Kim, C.; Park, C.
    Novel enzymatic production of trehalose from sucrose using amylosucrase and maltooligosyltrehalose synthase-trehalohydrolase (2011), World J. Microbiol. Biotechnol., 27, 2851-2856.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of GST-tagged ASase in Escherichia coli strain BL21 (DE3) Deinococcus geothermalis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
73000
-
x * 73214, sequence calculation, x * 73000, recombinant detagged enzyme, SDS-PAGE Deinococcus geothermalis
73214
-
x * 73214, sequence calculation, x * 73000, recombinant detagged enzyme, SDS-PAGE Deinococcus geothermalis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sucrose + [(1->4)-alpha-D-glucosyl]n Deinococcus geothermalis
-
D-fructose + [(1->4)-alpha-D-glucosyl]n+1
-
?

Organism

Organism UniProt Comment Textmining
Deinococcus geothermalis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant GST-tagged ASase from Escherichia coli strain BL21 (DE3) by glutathione affinity chromatography, the tag is cleaved off by thrombin Deinococcus geothermalis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.8
-
sucrose hydrolysis, purified recombinant GST-tagged DgAS, pH 7.0, 37°C Deinococcus geothermalis
5
-
sucrose hydrolysis, purified recombinant detagged DgAS, pH 7.0, 37°C Deinococcus geothermalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzymatic production of trehalose from sucrose using amylosucrase and maltooligosyltrehalose synthase-trehalohydrolase, overview Deinococcus geothermalis ?
-
?
sucrose + [(1->4)-alpha-D-glucosyl]n
-
Deinococcus geothermalis D-fructose + [(1->4)-alpha-D-glucosyl]n+1
-
?

Subunits

Subunits Comment Organism
? x * 73214, sequence calculation, x * 73000, recombinant detagged enzyme, SDS-PAGE Deinococcus geothermalis

Synonyms

Synonyms Comment Organism
ASASE
-
Deinococcus geothermalis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Deinococcus geothermalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Deinococcus geothermalis