Cloned (Comment) | Organism |
---|---|
gene acas, locus CP001341, DNA and amino acid sequence determination and analysis, expression of C-terminally His6-tagged enzyme | Pseudarthrobacter chlorophenolicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
71705 | - |
1 * 72000, SDS-PAGE, 1 * 71705, sequence calculation | Pseudarthrobacter chlorophenolicus |
72000 | - |
gel filtration | Pseudarthrobacter chlorophenolicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudarthrobacter chlorophenolicus | - |
gene acas encoding an amylosucrase | - |
Pseudarthrobacter chlorophenolicus A6 / DSM 12829 | - |
gene acas encoding an amylosucrase | - |
Purification (Comment) | Organism |
---|---|
recombinant C-terminally His6-tagged enzyme by nickel affinity chromatography | Pseudarthrobacter chlorophenolicus |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 72000, SDS-PAGE, 1 * 71705, sequence calculation | Pseudarthrobacter chlorophenolicus |
Synonyms | Comment | Organism |
---|---|---|
ACAS | - |
Pseudarthrobacter chlorophenolicus |
ASASE | - |
Pseudarthrobacter chlorophenolicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Pseudarthrobacter chlorophenolicus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 50 | active at | Pseudarthrobacter chlorophenolicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
the purified recombinant His-tagged ACAS is stable up to 40°C, but rapid loss of activity above, half-life is 1.74 h | Pseudarthrobacter chlorophenolicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Pseudarthrobacter chlorophenolicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | high activity range | Pseudarthrobacter chlorophenolicus |
General Information | Comment | Organism |
---|---|---|
evolution | amylosucrase belongs to the glycoside hydrolase family 13, GH 13 | Pseudarthrobacter chlorophenolicus |