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Literature summary for 2.4.1.4 extracted from

  • Seo, D.H.; Jung, J.H.; Choi, H.C.; Cho, H.K.; Kim, H.H.; Ha, S.J.; Yoo, S.H.; Cha, J.; Park, C.S.
    Functional expression of amylosucrase, a glucan-synthesizing enzyme, from Arthrobacter chlorophenolicus A6 (2012), J. Microbiol. Biotechnol., 22, 1253-1257.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene acas, locus CP001341, DNA and amino acid sequence determination and analysis, expression of C-terminally His6-tagged enzyme Pseudarthrobacter chlorophenolicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
71705
-
1 * 72000, SDS-PAGE, 1 * 71705, sequence calculation Pseudarthrobacter chlorophenolicus
72000
-
gel filtration Pseudarthrobacter chlorophenolicus

Organism

Organism UniProt Comment Textmining
Pseudarthrobacter chlorophenolicus
-
gene acas encoding an amylosucrase
-
Pseudarthrobacter chlorophenolicus A6 / DSM 12829
-
gene acas encoding an amylosucrase
-

Purification (Commentary)

Purification (Comment) Organism
recombinant C-terminally His6-tagged enzyme by nickel affinity chromatography Pseudarthrobacter chlorophenolicus

Subunits

Subunits Comment Organism
monomer 1 * 72000, SDS-PAGE, 1 * 71705, sequence calculation Pseudarthrobacter chlorophenolicus

Synonyms

Synonyms Comment Organism
ACAS
-
Pseudarthrobacter chlorophenolicus
ASASE
-
Pseudarthrobacter chlorophenolicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
Pseudarthrobacter chlorophenolicus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 50 active at Pseudarthrobacter chlorophenolicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
the purified recombinant His-tagged ACAS is stable up to 40°C, but rapid loss of activity above, half-life is 1.74 h Pseudarthrobacter chlorophenolicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Pseudarthrobacter chlorophenolicus

pH Range

pH Minimum pH Maximum Comment Organism
7 9 high activity range Pseudarthrobacter chlorophenolicus

General Information

General Information Comment Organism
evolution amylosucrase belongs to the glycoside hydrolase family 13, GH 13 Pseudarthrobacter chlorophenolicus