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Literature summary for 2.4.1.4 extracted from

  • Jung, J.H.; Seo, D.H.; Ha, S.J.; Song, M.C.; Cha, J.; Yoo, S.H.; Kim, T.J.; Baek, N.I.; Baik, M.Y.; Park, C.S.
    Enzymatic synthesis of salicin glycosides through transglycosylation catalyzed by amylosucrases from Deinococcus geothermalis and Neisseria polysaccharea (2009), Carbohydr. Res., 344, 1612-1619.
    View publication on PubMed

Application

Application Comment Organism
synthesis the enzyme can be used for synthesis of salicin glycosides with sucrose serving as the glucopyranosyl donor and salicin as the acceptor molecule Deinococcus geothermalis
synthesis the enzyme can efficiently be used for synthesis of salicin glycosides with sucrose serving as the glucopyranosyl donor and salicin as the acceptor molecule Neisseria polysaccharea

Cloned(Commentary)

Cloned (Comment) Organism
expression of GST-tagged DGAS in Escherichia coli strain BL21 Deinococcus geothermalis
expression of His-tagged NPAS in Escherichia coli strain BL21 Neisseria polysaccharea

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Deinococcus geothermalis the enzyme catalyze the synthesis of an alpha-(1,4)-linked glucan polymer from sucrose instead of an expensive activated sugar, such as ADP- or UDP-glucose ?
-
?
additional information Neisseria polysaccharea the enzyme catalyze the synthesis of an alpha-(1,4)-linked glucan polymer from sucrose instead of an expensive activated sugar, such as ADP- or UDP-glucose ?
-
?

Organism

Organism UniProt Comment Textmining
Deinococcus geothermalis
-
strain DSM 11300
-
Neisseria polysaccharea Q9ZEU2 strain ATCC 43768
-

Purification (Commentary)

Purification (Comment) Organism
recombinant GST-tagged DGAS from Escherichia coli strain BL21 by glutathione affinity chromatography Deinococcus geothermalis
recombinant His-tagged NPAS from Escherichia coli strain BL21 by nickel affinity chromatography Neisseria polysaccharea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme catalyze the synthesis of an alpha-(1,4)-linked glucan polymer from sucrose instead of an expensive activated sugar, such as ADP- or UDP-glucose Deinococcus geothermalis ?
-
?
additional information the enzyme catalyze the synthesis of an alpha-(1,4)-linked glucan polymer from sucrose instead of an expensive activated sugar, such as ADP- or UDP-glucose Neisseria polysaccharea ?
-
?
additional information the hydrolysis reaction is not a rate-limiting step to perform transglycosylation in rDGAS Deinococcus geothermalis ?
-
?
sucrose + salicin synthesis of salicin glycosides with sucrose serving as the glucopyranosyl donor and salicin as the acceptor molecule, DGAS specifically synthesizes only one salicin transglycosylation product Deinococcus geothermalis D-fructose + alpha-D-glucopyranosyl-(1,4)-salicin product determination by NMR and TLC ?
sucrose + salicin synthesis of salicin glycosides with sucrose serving as the glucopyranosyl donor and salicin as the acceptor molecule Neisseria polysaccharea D-fructose + alpha-D-glucopyranosyl-(1,4)-salicin + alpha-D-glucopyranosyl-(1,4)-alpha-D-glucopyranosyl-(1,4)-salicin i.e. glucosyl salicin and maltosyl salicin, identification by NMR and TLC analysis ?

Synonyms

Synonyms Comment Organism
ASASE
-
Deinococcus geothermalis
ASASE
-
Neisseria polysaccharea
DGAS
-
Deinococcus geothermalis
More the enzyme is a member of family 13 of the glycoside hydrolases Deinococcus geothermalis
More the enzyme is a member of family 13 of the glycoside hydrolases Neisseria polysaccharea
NPAS
-
Neisseria polysaccharea

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
recombinant enzyme Neisseria polysaccharea
47
-
recombinant enzyme Deinococcus geothermalis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 50 recombinant enzyme, activity range, temperature profile, overview Neisseria polysaccharea
20 57 recombinant enzyme, activity range, 80% of maximal activity at 35-45°C, 20% at 50°C, temperature profile, overview Deinococcus geothermalis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
half-life of NPAS is 21 h Neisseria polysaccharea
50
-
half-life of NPAS below 1 min Neisseria polysaccharea
55
-
DGAS shows a half-life of 6.8 h at 55°C Deinococcus geothermalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
recombinant enzyme Neisseria polysaccharea
8
-
recombinant enzyme Deinococcus geothermalis