Application | Comment | Organism |
---|---|---|
synthesis | the enzyme can be used for synthesis of salicin glycosides with sucrose serving as the glucopyranosyl donor and salicin as the acceptor molecule | Deinococcus geothermalis |
synthesis | the enzyme can efficiently be used for synthesis of salicin glycosides with sucrose serving as the glucopyranosyl donor and salicin as the acceptor molecule | Neisseria polysaccharea |
Cloned (Comment) | Organism |
---|---|
expression of GST-tagged DGAS in Escherichia coli strain BL21 | Deinococcus geothermalis |
expression of His-tagged NPAS in Escherichia coli strain BL21 | Neisseria polysaccharea |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Deinococcus geothermalis | the enzyme catalyze the synthesis of an alpha-(1,4)-linked glucan polymer from sucrose instead of an expensive activated sugar, such as ADP- or UDP-glucose | ? | - |
? | |
additional information | Neisseria polysaccharea | the enzyme catalyze the synthesis of an alpha-(1,4)-linked glucan polymer from sucrose instead of an expensive activated sugar, such as ADP- or UDP-glucose | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Deinococcus geothermalis | - |
strain DSM 11300 | - |
Neisseria polysaccharea | Q9ZEU2 | strain ATCC 43768 | - |
Purification (Comment) | Organism |
---|---|
recombinant GST-tagged DGAS from Escherichia coli strain BL21 by glutathione affinity chromatography | Deinococcus geothermalis |
recombinant His-tagged NPAS from Escherichia coli strain BL21 by nickel affinity chromatography | Neisseria polysaccharea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme catalyze the synthesis of an alpha-(1,4)-linked glucan polymer from sucrose instead of an expensive activated sugar, such as ADP- or UDP-glucose | Deinococcus geothermalis | ? | - |
? | |
additional information | the enzyme catalyze the synthesis of an alpha-(1,4)-linked glucan polymer from sucrose instead of an expensive activated sugar, such as ADP- or UDP-glucose | Neisseria polysaccharea | ? | - |
? | |
additional information | the hydrolysis reaction is not a rate-limiting step to perform transglycosylation in rDGAS | Deinococcus geothermalis | ? | - |
? | |
sucrose + salicin | synthesis of salicin glycosides with sucrose serving as the glucopyranosyl donor and salicin as the acceptor molecule, DGAS specifically synthesizes only one salicin transglycosylation product | Deinococcus geothermalis | D-fructose + alpha-D-glucopyranosyl-(1,4)-salicin | product determination by NMR and TLC | ? | |
sucrose + salicin | synthesis of salicin glycosides with sucrose serving as the glucopyranosyl donor and salicin as the acceptor molecule | Neisseria polysaccharea | D-fructose + alpha-D-glucopyranosyl-(1,4)-salicin + alpha-D-glucopyranosyl-(1,4)-alpha-D-glucopyranosyl-(1,4)-salicin | i.e. glucosyl salicin and maltosyl salicin, identification by NMR and TLC analysis | ? |
Synonyms | Comment | Organism |
---|---|---|
ASASE | - |
Deinococcus geothermalis |
ASASE | - |
Neisseria polysaccharea |
DGAS | - |
Deinococcus geothermalis |
More | the enzyme is a member of family 13 of the glycoside hydrolases | Deinococcus geothermalis |
More | the enzyme is a member of family 13 of the glycoside hydrolases | Neisseria polysaccharea |
NPAS | - |
Neisseria polysaccharea |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
recombinant enzyme | Neisseria polysaccharea |
47 | - |
recombinant enzyme | Deinococcus geothermalis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 50 | recombinant enzyme, activity range, temperature profile, overview | Neisseria polysaccharea |
20 | 57 | recombinant enzyme, activity range, 80% of maximal activity at 35-45°C, 20% at 50°C, temperature profile, overview | Deinococcus geothermalis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
half-life of NPAS is 21 h | Neisseria polysaccharea |
50 | - |
half-life of NPAS below 1 min | Neisseria polysaccharea |
55 | - |
DGAS shows a half-life of 6.8 h at 55°C | Deinococcus geothermalis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
recombinant enzyme | Neisseria polysaccharea |
8 | - |
recombinant enzyme | Deinococcus geothermalis |