Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in Escherichia coli strain JM109 | Deinococcus radiodurans |
Protein Variants | Comment | Organism |
---|---|---|
A170V/Q353L | random mutagenesis, the mutant shows 3.5fold increased thermostabilty at 50°C compared to the wild-type enzyme | Deinococcus radiodurans |
additional information | random mutagenesis and screening for enzyme variants with increased thermostability, molecular dynamics simulations, overview | Deinococcus radiodurans |
P351S | random mutagenesis, the mutant shows 8fold increased thermostabilty at 50°C compared to the wild-type enzyme | Deinococcus radiodurans |
R20C/A451T | random mutagenesis, the mutant shows 10fold increased thermostabilty at 50°C compared to the wild-type enzyme | Deinococcus radiodurans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Deinococcus radiodurans | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | amylosucrase is a transglucosidase that catalyzes amylose-like polymer synthesis from sucrose substrate | Deinococcus radiodurans | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
36 | - |
recombinant wild-type enzyme and mutant P351S | Deinococcus radiodurans |
40 | - |
recombinant mutants A170V/Q353L and R20C/A451T | Deinococcus radiodurans |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 50 | - |
Deinococcus radiodurans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
half-life of the recombinant wild-type enzyme is 3 min, and of mutant R20C/A451T 32 min, of mutant A170V/Q353L 10 min, and of mutant P351S 24 min | Deinococcus radiodurans |