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Literature summary for 2.4.1.4 extracted from

  • Emond, S.; Andre, I.; Jaziri, K.; Potocki-Veronese, G.; Mondon, P.; Bouayadi, K.; Kharrat, H.; Monsan, P.; Remaud-Simeon, M.
    Combinatorial engineering to enhance thermostability of amylosucrase (2008), Protein Sci., 17, 967-976.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant enzymes in Escherichia coli strain JM109 Deinococcus radiodurans

Protein Variants

Protein Variants Comment Organism
A170V/Q353L random mutagenesis, the mutant shows 3.5fold increased thermostabilty at 50°C compared to the wild-type enzyme Deinococcus radiodurans
additional information random mutagenesis and screening for enzyme variants with increased thermostability, molecular dynamics simulations, overview Deinococcus radiodurans
P351S random mutagenesis, the mutant shows 8fold increased thermostabilty at 50°C compared to the wild-type enzyme Deinococcus radiodurans
R20C/A451T random mutagenesis, the mutant shows 10fold increased thermostabilty at 50°C compared to the wild-type enzyme Deinococcus radiodurans

Organism

Organism UniProt Comment Textmining
Deinococcus radiodurans
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information amylosucrase is a transglucosidase that catalyzes amylose-like polymer synthesis from sucrose substrate Deinococcus radiodurans ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
36
-
recombinant wild-type enzyme and mutant P351S Deinococcus radiodurans
40
-
recombinant mutants A170V/Q353L and R20C/A451T Deinococcus radiodurans

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 50
-
Deinococcus radiodurans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
half-life of the recombinant wild-type enzyme is 3 min, and of mutant R20C/A451T 32 min, of mutant A170V/Q353L 10 min, and of mutant P351S 24 min Deinococcus radiodurans