Cloned (Comment) | Organism |
---|---|
gene dgas, DNA and amino acid sequence determination and analysis, expression of GST-tagged enzyme in Escherichia coli strain JM109 | Deinococcus geothermalis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Deinococcus geothermalis | amylosucrase is a transglucosidase that catalyses the synthesis of an amylose-type polymer from sucrose, an abundant agro-resource | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Deinococcus geothermalis | - |
gene dgas | - |
Purification (Comment) | Organism |
---|---|
recombinant GST-tagged enzyme from Escherichia coli strain JM109 by glutathione affinity chromatography | Deinococcus geothermalis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
44 | - |
purified recombinant enzyme | Deinococcus geothermalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | amylosucrase is a transglucosidase that catalyses the synthesis of an amylose-type polymer from sucrose, an abundant agro-resource | Deinococcus geothermalis | ? | - |
? | |
additional information | the purified recombinant enzyme produces an alpha-glucan at 50°C, with an average degree of polymerization of 45 and a polymerization yield of 76% | Deinococcus geothermalis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | three-dimensional modelling, overview | Deinococcus geothermalis |
Synonyms | Comment | Organism |
---|---|---|
DGAS | - |
Deinococcus geothermalis |
More | the enzyme belongs to the glycoside-hydrolase, GH, family 13 | Deinococcus geothermalis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
recombinant enzyme | Deinococcus geothermalis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
purified recombinant enzyme, half-life is 26 h, denaturation kinetics, overview | Deinococcus geothermalis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Deinococcus geothermalis |