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Literature summary for 2.4.1.4 extracted from

  • Emond, S.; Mondeil, S.; Jaziri, K.; Andre, I.; Monsan, P.; Remaud-Simeon, M.; Potocki-Veronese, G.
    Cloning, purification and characterization of a thermostable amylosucrase from Deinococcus geothermalis (2008), FEMS Microbiol. Lett., 285, 25-32.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene dgas, DNA and amino acid sequence determination and analysis, expression of GST-tagged enzyme in Escherichia coli strain JM109 Deinococcus geothermalis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Deinococcus geothermalis amylosucrase is a transglucosidase that catalyses the synthesis of an amylose-type polymer from sucrose, an abundant agro-resource ?
-
?

Organism

Organism UniProt Comment Textmining
Deinococcus geothermalis
-
gene dgas
-

Purification (Commentary)

Purification (Comment) Organism
recombinant GST-tagged enzyme from Escherichia coli strain JM109 by glutathione affinity chromatography Deinococcus geothermalis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
44
-
purified recombinant enzyme Deinococcus geothermalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information amylosucrase is a transglucosidase that catalyses the synthesis of an amylose-type polymer from sucrose, an abundant agro-resource Deinococcus geothermalis ?
-
?
additional information the purified recombinant enzyme produces an alpha-glucan at 50°C, with an average degree of polymerization of 45 and a polymerization yield of 76% Deinococcus geothermalis ?
-
?

Subunits

Subunits Comment Organism
More three-dimensional modelling, overview Deinococcus geothermalis

Synonyms

Synonyms Comment Organism
DGAS
-
Deinococcus geothermalis
More the enzyme belongs to the glycoside-hydrolase, GH, family 13 Deinococcus geothermalis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
recombinant enzyme Deinococcus geothermalis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
purified recombinant enzyme, half-life is 26 h, denaturation kinetics, overview Deinococcus geothermalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Deinococcus geothermalis