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Literature summary for 2.4.1.4 extracted from

  • van der Veen, B.A.; Potocki-Veronese, G.; Albenne, C.; Joucla, G.; Monsan, P.; Remaud-Simeon, M.
    Combinatorial engineering to enhance amylosucrase performance: construction, selection, and screening of variant libraries for increased activity (2004), FEBS Lett., 560, 91-97.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
potential use for the synthesis or the modification of polysaccharides is limited by its low catalytic efficiency on sucrose alone, its low stability, and its side reactions resulting in sucrose isomer formation. Development of a zero background expression cloning strategy for the generation of large variant libraries, a selection mechanism to discard inactive variants, and a screening method for identification of interesting clones Neisseria polysaccharea

Protein Variants

Protein Variants Comment Organism
synthesis potential use for the synthesis or the modification of polysaccharides is limited by its low catalytic efficiency on sucrose alone, its low stability, and its side reactions resulting in sucrose isomer formation. Development of a zero background expression cloning strategy for the generation of large variant libraries, a selection mechanism to discard inactive variants, and a screening method for identification of interesting clones Neisseria polysaccharea

Organism

Organism UniProt Comment Textmining
Neisseria polysaccharea
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