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Literature summary for 2.4.1.390 extracted from

  • Yang, Y.; Sun, Y.; Zhang, T.; Hamaker, B.R.; Miao, M.
    Insights into the catalytic properties of 4,3-alpha-glucanotransferase to guide the biofabrication of alpha-glucans with low digestibility (2024), Food Funct., 15, 8274-8285.
    View publication on PubMed
Search for more literature for 2.4.1.390

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Limosilactobacillus fermentum

Inhibitors

Inhibitors Comment Organism Structure
Al3+
-
 Limosilactobacillus fermentum
Co2+
-
 Limosilactobacillus fermentum
Cu2+
-
 Limosilactobacillus fermentum
Fe2+
-
 Limosilactobacillus fermentum
Pb2+
-
 Limosilactobacillus fermentum

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 1 mM, stimulates activity by 2.22fold Limosilactobacillus fermentum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
100000
-
 SDS-PAGE Limosilactobacillus fermentum

Organism

Organism UniProt Comment Textmining
Limosilactobacillus fermentum A0A1D7ZV76 cf. EC 2.4.1.5; cf. EC 2.4.1.5
-

Purification (Commentary)

Purification (Comment) Organism
-
 Limosilactobacillus fermentum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
high-amylose maize starch
-
 Limosilactobacillus fermentum ?
-
 ?
maize starch
-
 Limosilactobacillus fermentum ?
-
 ?
additional information 4,3-alpha-GTase has a stronger affinity for linear starch chains than for branched glucan chains. The affinity of 4,3-alpha-GTase for alpha-1,4 linked glucan chains is positively correlated with the degree of polymerization of the substrate. Oligosaccharides are the receptor molecules of 4,3-alpha-GTase. Therefore the hydrolytic activity of the enzyme is preferentially presented when the enzyme was incubated with long-chain alpha-1,4 glucans. Longchain alpha-1,4 linked glucan chains are the preferred donor molecules for the enzymatic transglycosylation reaction, and the higher degree of polymerization value results in higher efficiency of the transglycosylation reaction Limosilactobacillus fermentum ?
-
-
waxy maize starch
-
 Limosilactobacillus fermentum ?
-
 ?

Subunits

Subunits Comment Organism
? x * 100000, SDS-PAGE Limosilactobacillus fermentum

Synonyms

Synonyms Comment Organism
4,3-alpha-GTase
-
 Limosilactobacillus fermentum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Limosilactobacillus fermentum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 50 more than 90% activity between 30°C-50°C Limosilactobacillus fermentum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 6 h, approximately 90% residual activity is retained Limosilactobacillus fermentum
45
-
 6 h, more than 60% residual activity is retained Limosilactobacillus fermentum
50
-
 6 h, more than 40% residual activity is retained Limosilactobacillus fermentum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Limosilactobacillus fermentum

pH Range

pH Minimum pH Maximum Comment Organism
5 6.5 more than 90% activity between pH 5.0-6.5 Limosilactobacillus fermentum