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Literature summary for 2.4.1.390 extracted from
- Extracellular recombinant production of 4,6 and 4,3 alpha-glucanotransferases in Lactococcus lactis (2023), Enzyme Microb. Technol., 164, 110175.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| 4,6-alpha-GTase (cf. EC 2.4.1.394) and 4,3-alpha-GTase genes from Limosilactobacillus reuteri E81 and Limosilactobacillus fermentum PFC282 respectively are transformed into the plasmid pLEB124 vector having the signal peptide usp45 under the P45 continuous promoter and are successfully expressed in Lactococcus lactis MG1363 | Limosilactobacillus fermentum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Limosilactobacillus fermentum | - |
- |
- |
| Limosilactobacillus fermentum PFC282 | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| maltoheptaose | - |
Limosilactobacillus fermentum | malto-oligosaccaride | - |
? |  |
| maltoheptaose | - |
Limosilactobacillus fermentum PFC282 | malto-oligosaccaride | - |
? | |
| maltohexaose | - |
Limosilactobacillus fermentum | malto-oligosaccaride | - |
? | |
| maltohexaose | - |
Limosilactobacillus fermentum PFC282 | malto-oligosaccaride | - |
? | |
| maltose | - |
Limosilactobacillus fermentum | malto-oligosaccaride | - |
? | |
| maltose | - |
Limosilactobacillus fermentum PFC282 | malto-oligosaccaride | - |
? | |
| starch | - |
Limosilactobacillus fermentum | malto-oligosaccaride | - |
? | |
| starch | - |
Limosilactobacillus fermentum PFC282 | malto-oligosaccaride | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 4,3-alpha-GTase | - |
Limosilactobacillus fermentum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Limosilactobacillus fermentum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Limosilactobacillus fermentum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | 7.5 | pH 3.5: about 45% of maximal activity, pH 7.5: about 45% of maximal activity | Limosilactobacillus fermentum |
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