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Literature summary for 2.4.1.390 extracted from

  • Gangoiti, J.; van Leeuwen, S.S.; Gerwig, G.J.; Duboux, S.; Vafiadi, C.; Pijning, T.; Dijkhuizen, L.
    4,3-alpha-Glucanotransferase, a novel reaction specificity in glycoside hydrolase family 70 and clan GH-H (2017), Sci. Rep., 7, 39761.
    View publication on PubMed
Search for more literature for 2.4.1.390

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Limosilactobacillus fermentum

Crystallization (Commentary)

Crystallization (Comment) Organism
homology modeling of structure Limosilactobacillus fermentum

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular predicted by in silico analysis, protein contains the classical Gram-positive N-terminal signal peptide of 39 amino acids Limosilactobacillus fermentum
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-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
amylose V Limosilactobacillus fermentum
-
 alpha-glucans with alternating alpha (1 ->3)/alpha (1->4)-linkages and with alpha(1->3,4) branching points + D-glucose
-
 ?
maltoheptaose Limosilactobacillus fermentum
-
 alpha-glucans with alternating alpha (1 ->3)/alpha (1->4)-linkages and with alpha(1->3,4) branching points + D-glucose
-
 ?
maltohexaose Limosilactobacillus fermentum
-
 alpha-glucans with alternating alpha (1 ->3)/alpha (1->4)-linkages and with alpha(1->3,4) branching points + D-glucose
-
 ?
maltopentaose Limosilactobacillus fermentum
-
 alpha-glucans with alternating alpha (1 ->3)/alpha (1->4)-linkages and with alpha(1->3,4) branching points + D-glucose
-
 ?
additional information Limosilactobacillus fermentum GtfB displays both hydrolysis and transglycosylase (disproportionation) activity on malto-oligosaccharides of DP 6 and 7. GtfB is not active on malto-oligosaccharides of DP below 4 and shows low disproportionating activity with maltopentaose. The enzyme fails to act on sucrose, panose, nigerose, and isomalto-oligosaccharides with DP2, DP3, and DP5 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Limosilactobacillus fermentum A0A1D7ZV76 cf. EC 2.4.1.5
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Purification (Commentary)

Purification (Comment) Organism
recombinant protein, purification from soluble fraction by metal chelate chromatography and anion-exchange chromatography Limosilactobacillus fermentum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amylose V
-
 Limosilactobacillus fermentum alpha-glucans with alternating alpha (1 ->3)/alpha (1->4)-linkages and with alpha(1->3,4) branching points + D-glucose
-
 ?
amylose V
-
 Limosilactobacillus fermentum alpha-glucans with alternating alpha (1 ->3)/alpha (1->4)-linkages and with alpha(1->3,4) branching points + maltose
-
 ?
maltoheptaose
-
 Limosilactobacillus fermentum alpha-glucans with alternating alpha (1 ->3)/alpha (1->4)-linkages and with alpha(1->3,4) branching points + D-glucose
-
 ?
maltoheptaose
-
 Limosilactobacillus fermentum alpha-glucans with alternating alpha (1 ->3)/alpha (1->4)-linkages and with alpha(1->3,4) branching points + maltose
-
 ?
maltohexaose
-
 Limosilactobacillus fermentum alpha-glucans with alternating alpha (1 ->3)/alpha (1->4)-linkages and with alpha(1->3,4) branching points + D-glucose
-
 ?
maltohexaose
-
 Limosilactobacillus fermentum alpha-glucans with alternating alpha (1 ->3)/alpha (1->4)-linkages and with alpha(1->3,4) branching points + maltose
-
 ?
maltopentaose
-
 Limosilactobacillus fermentum alpha-glucans with alternating alpha (1 ->3)/alpha (1->4)-linkages and with alpha(1->3,4) branching points + D-glucose
-
 ?
maltopentaose
-
 Limosilactobacillus fermentum alpha-glucans with alternating alpha (1 ->3)/alpha (1->4)-linkages and with alpha(1->3,4) branching points + maltose
-
 ?
additional information GtfB displays both hydrolysis and transglycosylase (disproportionation) activity on malto-oligosaccharides of DP 6 and 7. GtfB is not active on malto-oligosaccharides of DP below 4 and shows low disproportionating activity with maltopentaose. The enzyme fails to act on sucrose, panose, nigerose, and isomalto-oligosaccharides with DP2, DP3, and DP5 Limosilactobacillus fermentum ?
-
 ?

Subunits

Subunits Comment Organism
? x * 180000, calculated from sequence Limosilactobacillus fermentum

Synonyms

Synonyms Comment Organism
GtfB
-
 Limosilactobacillus fermentum
LACFE_CDS0221
-
 Limosilactobacillus fermentum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Limosilactobacillus fermentum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 60
-
 Limosilactobacillus fermentum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 enzyme shows low stability at temperatures above 45°C in 20 mM Tris-HCl buffer pH 8.0 Limosilactobacillus fermentum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Limosilactobacillus fermentum

pH Range

pH Minimum pH Maximum Comment Organism
4 6 more than 80% of maximum activity Limosilactobacillus fermentum

General Information

General Information Comment Organism
physiological function enzyme acts as a 4,3-alpha-glucanotransferase, converting amylose into a new type of alpha-glucan with alternating alpha (1 ->3)/alpha (1->4)-linkages and with alpha(1->3,4) branching points Limosilactobacillus fermentum