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Literature summary for 2.4.1.388 extracted from

  • De Doncker, M.; De Graeve, C.; Franceus, J.; Beerens, K.; Kren, V.; Pelantova, H.; Vercauteren, R.; Desmet, T.
    Exploration of GH94 sequence space for enzyme discovery reveals a novel glucosylgalactose phosphorylase specificity (2021), ChemBioChem, 22, 3319-3325 .
    View publication on PubMed
Search for more literature for 2.4.1.388

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7
-
 beta-D-glucosyl-(1->4)-D-galactose at pH 6.5 and 40°C Paenibacillus polymyxa
5
-
 alpha-D-glucopyranose 1-phosphate at pH 6.5 and 40°C Paenibacillus polymyxa
6.7
-
 D-galactopyranose at pH 6.5 and 40°C Paenibacillus polymyxa
17
-
 phosphate at pH 6.5 and 40°C Paenibacillus polymyxa
39
-
 L-arabinose at pH 6.5 and 40°C Paenibacillus polymyxa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
alpha-D-glucopyranose 1-phosphate + D-galactopyranose Paenibacillus polymyxa
-
 beta-D-glucosyl-(1->4)-D-galactose + phosphate
-
 r
beta-D-glucosyl-(1->4)-D-galactose + phosphate Paenibacillus polymyxa
-
 alpha-D-glucopyranose 1-phosphate + D-galactopyranose
-
 r

Organism

Organism UniProt Comment Textmining
Paenibacillus polymyxa
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-D-glucopyranose 1-phosphate + D-galactopyranose
-
 Paenibacillus polymyxa beta-D-glucosyl-(1->4)-D-galactose + phosphate
-
 r
alpha-D-glucopyranose 1-phosphate + L-arabinose
-
 Paenibacillus polymyxa ?
-
 r
beta-D-glucosyl-(1->4)-D-galactose + phosphate
-
 Paenibacillus polymyxa alpha-D-glucopyranose 1-phosphate + D-galactopyranose
-
 r

Subunits

Subunits Comment Organism
? x * 89400, calculated from amino acid sequence Paenibacillus polymyxa

Synonyms

Synonyms Comment Organism
4-O-beta-D-glucosyl-D-galactose phosphorylase
-
 Paenibacillus polymyxa
GGalP
-
 Paenibacillus polymyxa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3 6 beta-D-glucosyl-(1->4)-D-galactose at pH 6.5 and 40°C Paenibacillus polymyxa
34
-
 phosphate at pH 6.5 and 40°C Paenibacillus polymyxa
57
-
 D-galactopyranose at pH 6.5 and 40°C Paenibacillus polymyxa
69
-
 alpha-D-glucopyranose 1-phosphate at pH 6.5 and 40°C Paenibacillus polymyxa
151
-
 L-arabinose at pH 6.5 and 40°C Paenibacillus polymyxa

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2
-
 phosphate at pH 6.5 and 40°C Paenibacillus polymyxa
3.9
-
 L-arabinose at pH 6.5 and 40°C Paenibacillus polymyxa
8.5
-
 D-galactopyranose at pH 6.5 and 40°C Paenibacillus polymyxa
13.3
-
 beta-D-glucosyl-(1->4)-D-galactose at pH 6.5 and 40°C Paenibacillus polymyxa
13.8
-
 alpha-D-glucopyranose 1-phosphate at pH 6.5 and 40°C Paenibacillus polymyxa