Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Triton X-100 | - |
Withania somnifera |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
(5alpha, 6beta,17alpha)-5,6,17,27-tetrahydroxywithanolide | pH 8.0, 30°C | Withania somnifera | |
0.02 | - |
UDP-glucose | pH 8.0, 30°C | Withania somnifera | |
0.04 | - |
estradiol | pH 8.0, 30°C | Withania somnifera | |
0.05 | - |
withanolide U | pH 8.0, 30°C | Withania somnifera | |
0.07 | - |
21beta-hydroxy progesterone | pH 8.0, 30°C | Withania somnifera | |
0.11 | - |
27-hydroxywithanone | pH 8.0, 30°C | Withania somnifera | |
0.11 | - |
withanolide A | pH 8.0, 30°C | Withania somnifera | |
0.17 | - |
testosterone | pH 8.0, 30°C | Withania somnifera | |
0.17 | - |
17alpha-hydroxywithaferin | pH 8.0, 30°C | Withania somnifera |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Withania somnifera | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52000 | - |
gel filtration, SDS-PAGE | Withania somnifera |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Withania somnifera | can conjugate glucose to C-27 position in the withanolide lactone ring and to higher positions in sterols using UDP-glucose as glucose donor, activity is enhanced following the application of salicylic acid and decreased rapidly on exposure of the plants to heat shock, suggesting a functional role of the enzyme in biotic and abiotic stresses | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Withania somnifera | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
UDP-alpha-D-glucose + a 27-hydroxysteroid = UDP + a sterol 27-beta-D-glucoside | enzyme transferes beta-D-glucose to beta-OH present at C-20, C-21 and C-27 positions in sterols/withanolides, provided the sterol posesses a 17beta-OH group. Does not glycosylate sterols at the C-3 position | Withania somnifera |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Withania somnifera | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | can conjugate glucose to C-27 position in the withanolide lactone ring and to higher positions in sterols using UDP-glucose as glucose donor, activity is enhanced following the application of salicylic acid and decreased rapidly on exposure of the plants to heat shock, suggesting a functional role of the enzyme in biotic and abiotic stresses | Withania somnifera | ? | - |
? | |
additional information | UDP-glucose can not be replaced by UDP-galactose | Withania somnifera | ? | - |
? | |
UDP-alpha-D-glucose + 17alpha-hydroxywithaferin | - |
Withania somnifera | UDP + 27-O-(beta-D-glucopyranosyl)-17alpha-hydroxywithaferin | - |
? | |
UDP-alpha-D-glucose + 17alpha-hydroxywithaferin A | 15% of the activity with testosterone as substrate | Withania somnifera | UDP + 27-O-(beta-D-glucopyranosyl)-17alpha-hydroxywithaferin A | - |
? | |
UDP-alpha-D-glucose + 21-hydroxyprogesterone | 13% of the activity with testosterone as substrate | Withania somnifera | UDP + 3,20-dioxopregn-4-en-21-yl beta-D-glucopyranoside | - |
? | |
UDP-alpha-D-glucose + 27-hydroxywithanone | 15% of the activity with testosterone as substrate | Withania somnifera | UDP + 27-O-(beta-D-glucopyranosyl)-27-hydroxywithanone | - |
? | |
UDP-alpha-D-glucose + 5alpha,6beta,17alpha,27-tetrahydroxywithanolide | 23% of the activity with testosterone as substrate | Withania somnifera | UDP + 27-O-(beta-D-glucopyranosyl)-5alpha,6beta,17alpha,27-tetrahydroxywithanolide | - |
? | |
UDP-alpha-D-glucose + hydrocortisone | 11% of the activity with testosterone as substrate | Withania somnifera | UDP + 11beta,17-dihydroxy-3,20-dioxopregn-4-en-21-yl beta-D-glucopyranoside | - |
? | |
UDP-alpha-D-glucose + withanolide A | 21% of the activity with testosterone as substrate | Withania somnifera | UDP + 20-O-(beta-D-glucopyranosyl)-withanolide A | - |
? | |
UDP-alpha-D-glucose + withanolide U | 17% of the activity with testosterone as substrate | Withania somnifera | UDP + 20-O-(beta-D-glucopyranosyl)-withanolide U | - |
? | |
UDP-glucose + estradiol | 41% of the activity with testosterone as substrate | Withania somnifera | UDP + ? | - |
? | |
UDP-glucose + testosterone | 100% of activity | Withania somnifera | UDP + testosterone-17-yl beta-D-glucopyranoside | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 52000, SDS-PAGE, native mass by gel filtration | Withania somnifera |
Synonyms | Comment | Organism |
---|---|---|
More | distinct from EC 2.4.1.173, does not glycosylate sterols at the C-3 position | Withania somnifera |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Withania somnifera |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Withania somnifera |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | half maximum activity at pH 7 and 9 | Withania somnifera |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Withania somnifera | chromatofocusing | - |
4.8 |