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Literature summary for 2.4.1.385 extracted from

  • Madina, B.R.; Sharma, L.K.; Chaturvedi, P.; Sangwan, R.S.; Tuli, R.
    Purification and characterization of a novel glucosyltransferase specific to 27beta-hydroxy steroidal lactones from Withania somnifera and its role in stress responses (2007), Biochim. Biophys. Acta, 1774, 1199-1207.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Triton X-100
-
Withania somnifera

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
(5alpha, 6beta,17alpha)-5,6,17,27-tetrahydroxywithanolide pH 8.0, 30°C Withania somnifera
0.02
-
UDP-glucose pH 8.0, 30°C Withania somnifera
0.04
-
estradiol pH 8.0, 30°C Withania somnifera
0.05
-
withanolide U pH 8.0, 30°C Withania somnifera
0.07
-
21beta-hydroxy progesterone pH 8.0, 30°C Withania somnifera
0.11
-
27-hydroxywithanone pH 8.0, 30°C Withania somnifera
0.11
-
withanolide A pH 8.0, 30°C Withania somnifera
0.17
-
testosterone pH 8.0, 30°C Withania somnifera
0.17
-
17alpha-hydroxywithaferin pH 8.0, 30°C Withania somnifera

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Withania somnifera 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52000
-
gel filtration, SDS-PAGE Withania somnifera

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Withania somnifera can conjugate glucose to C-27 position in the withanolide lactone ring and to higher positions in sterols using UDP-glucose as glucose donor, activity is enhanced following the application of salicylic acid and decreased rapidly on exposure of the plants to heat shock, suggesting a functional role of the enzyme in biotic and abiotic stresses ?
-
?

Organism

Organism UniProt Comment Textmining
Withania somnifera
-
-
-

Reaction

Reaction Comment Organism Reaction ID
UDP-alpha-D-glucose + a 27-hydroxysteroid = UDP + a sterol 27-beta-D-glucoside enzyme transferes beta-D-glucose to beta-OH present at C-20, C-21 and C-27 positions in sterols/withanolides, provided the sterol posesses a 17beta-OH group. Does not glycosylate sterols at the C-3 position Withania somnifera

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Withania somnifera
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information can conjugate glucose to C-27 position in the withanolide lactone ring and to higher positions in sterols using UDP-glucose as glucose donor, activity is enhanced following the application of salicylic acid and decreased rapidly on exposure of the plants to heat shock, suggesting a functional role of the enzyme in biotic and abiotic stresses Withania somnifera ?
-
?
additional information UDP-glucose can not be replaced by UDP-galactose Withania somnifera ?
-
?
UDP-alpha-D-glucose + 17alpha-hydroxywithaferin
-
Withania somnifera UDP + 27-O-(beta-D-glucopyranosyl)-17alpha-hydroxywithaferin
-
?
UDP-alpha-D-glucose + 17alpha-hydroxywithaferin A 15% of the activity with testosterone as substrate Withania somnifera UDP + 27-O-(beta-D-glucopyranosyl)-17alpha-hydroxywithaferin A
-
?
UDP-alpha-D-glucose + 21-hydroxyprogesterone 13% of the activity with testosterone as substrate Withania somnifera UDP + 3,20-dioxopregn-4-en-21-yl beta-D-glucopyranoside
-
?
UDP-alpha-D-glucose + 27-hydroxywithanone 15% of the activity with testosterone as substrate Withania somnifera UDP + 27-O-(beta-D-glucopyranosyl)-27-hydroxywithanone
-
?
UDP-alpha-D-glucose + 5alpha,6beta,17alpha,27-tetrahydroxywithanolide 23% of the activity with testosterone as substrate Withania somnifera UDP + 27-O-(beta-D-glucopyranosyl)-5alpha,6beta,17alpha,27-tetrahydroxywithanolide
-
?
UDP-alpha-D-glucose + hydrocortisone 11% of the activity with testosterone as substrate Withania somnifera UDP + 11beta,17-dihydroxy-3,20-dioxopregn-4-en-21-yl beta-D-glucopyranoside
-
?
UDP-alpha-D-glucose + withanolide A 21% of the activity with testosterone as substrate Withania somnifera UDP + 20-O-(beta-D-glucopyranosyl)-withanolide A
-
?
UDP-alpha-D-glucose + withanolide U 17% of the activity with testosterone as substrate Withania somnifera UDP + 20-O-(beta-D-glucopyranosyl)-withanolide U
-
?
UDP-glucose + estradiol 41% of the activity with testosterone as substrate Withania somnifera UDP + ?
-
?
UDP-glucose + testosterone 100% of activity Withania somnifera UDP + testosterone-17-yl beta-D-glucopyranoside
-
?

Subunits

Subunits Comment Organism
monomer 1 * 52000, SDS-PAGE, native mass by gel filtration Withania somnifera

Synonyms

Synonyms Comment Organism
More distinct from EC 2.4.1.173, does not glycosylate sterols at the C-3 position Withania somnifera

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
Withania somnifera

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Withania somnifera

pH Range

pH Minimum pH Maximum Comment Organism
7 9 half maximum activity at pH 7 and 9 Withania somnifera

pI Value

Organism Comment pI Value Maximum pI Value
Withania somnifera chromatofocusing
-
4.8