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Literature summary for 2.4.1.376 extracted from

  • Li, Z.; Fischer, M.; Satkunarajah, M.; Zhou, D.; Withers, S.; Rini, J.
    Structural basis of Notch O-glucosylation and O-xylosylation by mammalian protein-O-glucosyltransferase 1 (POGLUT1) (2017), Nat. Commun., 8, 185 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.376

Crystallization (Commentary)

Crystallization (Comment) Organism
enzyme in complexes with three different EGF-like domains and either UDP, UDP-2-deoxy-2-fluoro-glucose or UDP-methyl-glucose, hanging drop vapor diffusion method, using 10-20% (w/v) PEG 5000 MME, 50 mM MES pH 6.5, 2-10 mM CaCl2, 0-250 mM NaCl, 5-10% (v/v) glycerol or 2-methyl-2,4-pentanediol Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
UDP-2-deoxy-2-fluoro-glucose
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-alpha-D-glucose + [protein with EGF-like domain]-L-serine Homo sapiens the enzyme can only glycosylate serine residues in the C1XSXPC2 motif UDP + [protein with EGF-like domain]-3-O-(beta-D-glucosyl)-L-serine
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q8NBL1
-
-

Purification (Commentary)

Purification (Comment) Organism
IgG Sepharose affinity chromatography, HiTrap Sepharose column chromatography, and Superdex 200 gel filtration Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-alpha-D-glucose + [factor VII protein with EGF-like domain 1]-L-serine
-
 Homo sapiens UDP + [factor VII protein with EGF-like domain 1]-3-O-(beta-D-glucosyl)-L-serine
-
 ?
UDP-alpha-D-glucose + [Notch1 protein with EGF-like domain 12]-L-serine the enzyme can only glycosylate serine residues in the C1XSXPC2 motif Homo sapiens UDP + [Notch1 protein with EGF-like domain 12]-3-O-(beta-D-glucosyl)-L-serine
-
 ?
UDP-alpha-D-glucose + [protein with EGF-like domain]-L-serine the enzyme can only glycosylate serine residues in the C1XSXPC2 motif Homo sapiens UDP + [protein with EGF-like domain]-3-O-(beta-D-glucosyl)-L-serine
-
 ?

Synonyms

Synonyms Comment Organism
POGLUT1
-
 Homo sapiens
protein-O-glucosyltransferase 1
-
 Homo sapiens
Rumi
-
 Homo sapiens