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Literature summary for 2.4.1.376 extracted from

  • Yu, H.; Takeuchi, H.; Takeuchi, M.; Liu, Q.; Kantharia, J.; Haltiwanger, R.; Li, H.
    Structural analysis of Notch-regulating Rumi reveals basis for pathogenic mutations (2016), Nat. Chem. Biol., 12, 735-740 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.376

Cloned(Commentary)

Cloned (Comment) Organism
expressed in HEK-293T cells Drosophila melanogaster

Crystallization (Commentary)

Crystallization (Comment) Organism
enzyme in binary or ternary complexes with factor IX EGF repeat residues 46-84 and UDP/UDP-alpha-D-glucose, hanging drop vapor diffusion method Drosophila melanogaster

Protein Variants

Protein Variants Comment Organism
A124F the mutation reduces the in vitro enzyme activity Drosophila melanogaster
A192F the mutation markedly reduces the in vitro enzyme activity Drosophila melanogaster
F122A the mutant retains only a trace amount of activity compared to the wild type enzyme Drosophila melanogaster
G189R inactive Drosophila melanogaster
G199V the mutant retains only a trace amount of activity compared to the wild type enzyme Drosophila melanogaster
P197A the mutation markedly reduces the in vitro enzyme activity Drosophila melanogaster
Q259A inactive Drosophila melanogaster
R245L the mutation markedly reduces the enzyme activity Drosophila melanogaster
R298W the mutation completely abolishes the enzyme activity in vitro Drosophila melanogaster
S231A the mutation markedly reduces the in vitro enzyme activity Drosophila melanogaster
T267I the mutation markedly reduces the enzyme activity Drosophila melanogaster

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-alpha-D-glucose + [factor IX protein with EGF-like domain]-L-serine Drosophila melanogaster
-
 UDP + [factor IX protein with EGF-like domain]-3-O-(beta-D-glucosyl)-L-serine
-
 ?
UDP-alpha-D-glucose + [Notch protein with EGF-like domain]-L-serine Drosophila melanogaster
-
 UDP + [Notch protein with EGF-like domain]-3-O-(beta-D-glucosyl)-L-serine
-
 ?

Organism

Organism UniProt Comment Textmining
Drosophila melanogaster
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography and Superdex 200 gel filtration Drosophila melanogaster

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-alpha-D-glucose + [factor IX protein with EGF-like domain]-L-serine
-
 Drosophila melanogaster UDP + [factor IX protein with EGF-like domain]-3-O-(beta-D-glucosyl)-L-serine
-
 ?
UDP-alpha-D-glucose + [Notch protein with EGF-like domain]-L-serine
-
 Drosophila melanogaster UDP + [Notch protein with EGF-like domain]-3-O-(beta-D-glucosyl)-L-serine
-
 ?

Synonyms

Synonyms Comment Organism
Rumi
-
 Drosophila melanogaster

General Information

General Information Comment Organism
metabolism the enzyme is essential for Notch signaling Drosophila melanogaster