Cloned (Comment) | Organism |
---|---|
expressed in HEK-293T cells | Drosophila melanogaster |
Crystallization (Comment) | Organism |
---|---|
enzyme in binary or ternary complexes with factor IX EGF repeat residues 46-84 and UDP/UDP-alpha-D-glucose, hanging drop vapor diffusion method | Drosophila melanogaster |
Protein Variants | Comment | Organism |
---|---|---|
A124F | the mutation reduces the in vitro enzyme activity | Drosophila melanogaster |
A192F | the mutation markedly reduces the in vitro enzyme activity | Drosophila melanogaster |
F122A | the mutant retains only a trace amount of activity compared to the wild type enzyme | Drosophila melanogaster |
G189R | inactive | Drosophila melanogaster |
G199V | the mutant retains only a trace amount of activity compared to the wild type enzyme | Drosophila melanogaster |
P197A | the mutation markedly reduces the in vitro enzyme activity | Drosophila melanogaster |
Q259A | inactive | Drosophila melanogaster |
R245L | the mutation markedly reduces the enzyme activity | Drosophila melanogaster |
R298W | the mutation completely abolishes the enzyme activity in vitro | Drosophila melanogaster |
S231A | the mutation markedly reduces the in vitro enzyme activity | Drosophila melanogaster |
T267I | the mutation markedly reduces the enzyme activity | Drosophila melanogaster |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-alpha-D-glucose + [factor IX protein with EGF-like domain]-L-serine | Drosophila melanogaster | - |
UDP + [factor IX protein with EGF-like domain]-3-O-(beta-D-glucosyl)-L-serine | - |
? | |
UDP-alpha-D-glucose + [Notch protein with EGF-like domain]-L-serine | Drosophila melanogaster | - |
UDP + [Notch protein with EGF-like domain]-3-O-(beta-D-glucosyl)-L-serine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Drosophila melanogaster | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA agarose column chromatography and Superdex 200 gel filtration | Drosophila melanogaster |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-alpha-D-glucose + [factor IX protein with EGF-like domain]-L-serine | - |
Drosophila melanogaster | UDP + [factor IX protein with EGF-like domain]-3-O-(beta-D-glucosyl)-L-serine | - |
? | |
UDP-alpha-D-glucose + [Notch protein with EGF-like domain]-L-serine | - |
Drosophila melanogaster | UDP + [Notch protein with EGF-like domain]-3-O-(beta-D-glucosyl)-L-serine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Rumi | - |
Drosophila melanogaster |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is essential for Notch signaling | Drosophila melanogaster |