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Literature summary for 2.4.1.375 extracted from

  • Matsumoto, N.; Takenaka, Y.; Wachananawat, B.; Kajiura, H.; Imai, T.; Ishimizu, T.
    Rhamnogalacturonan I galactosyltransferase Detection of enzyme activity and its hyperactivation (2019), Plant Physiol. Biochem., 142, 173-178 .
    View publication on PubMed
Search for more literature for 2.4.1.375

Activating Compound

Activating Compound Comment Organism Structure
benzalkonium chloride 3 mM benzalkonium chloride increases the enzyme activity 14fold Vigna angularis
Cetylpyridinium chloride 3 mM cetylpyridinium chloride increases the enzyme activity about 25fold Vigna angularis
cetyltrimethylammonium bromide 3 mM cetyltrimethylammonium bromide increases the enzyme activity 29fold Vigna angularis
polyallylamine 0.01 mM polyallylamine increases the enzyme activity 40fold Vigna angularis

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ complete inhibition at 3 mM Vigna angularis
Fe2+ complete inhibition at 3 mM Vigna angularis
NaCl complete inhibition at 300 mM Vigna angularis
Zn2+ complete inhibition at 3 mM Vigna angularis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.12
-
 GR10-PA enzyme hyperactivated with 3 mM benzalkonium chloride, at pH 7.0 and 30°C Vigna angularis
0.2
-
 UDP-alpha-D-galactose enzyme hyperactivated with 3 mM benzalkonium chloride, at pH 7.0 and 30°C Vigna angularis
0.78
-
 GR10-PA at pH 7.0 and 30°C Vigna angularis
4
-
 UDP-alpha-D-galactose at pH 7.0 and 30°C Vigna angularis

Localization

Localization Comment Organism GeneOntology No. Textmining
Golgi apparatus
-
 Vigna angularis 5794
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ highest activity in the presence of 3 mM Mn2+ Vigna angularis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-alpha-D-galactose + rhamnogalacturonan I Vigna angularis
-
 UDP + 1,4-beta-galactan side chains in rhamnogalacturonan I
-
 ?

Organism

Organism UniProt Comment Textmining
Vigna angularis
-
 azuki bean, cultivar Erimo-wase
-

Source Tissue

Source Tissue Comment Organism Textmining
epicotyl
-
 Vigna angularis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no activity with RG3-PA, GR84-PA RG5-PA, GR6-PA, RG7-PA, and GR8-PA Vigna angularis ?
-
-
UDP-alpha-D-galactose + GR10-PA i.e. alpha-D-galacturonosyl-(1->2)-alpha-L-rhamnosyl-(1->4)-alpha-D-galacturonosyl-(1->2)-alpha-L-rhamnosyl-(1->4)-alpha-D-galacturonosyl-(1->2)-alpha-L-rhamnosyl-(1->4)-alpha-D-galacturonosyl-(1->2)-alpha-L-rhamnosyl-(1->4)-alpha-D-galacturonosyl-(1->2)-alpha-L-rhamnosyl-pyridylamino Vigna angularis UDP + GR10(Gal)-PA
-
 ?
UDP-alpha-D-galactose + GR12-PA highest activity Vigna angularis UDP + GR12(Gal)-PA
-
 ?
UDP-alpha-D-galactose + GR14-PA second highest activity Vigna angularis UDP + GR14(Gal)-PA
-
 ?
UDP-alpha-D-galactose + RG9-PA
-
 Vigna angularis UDP + RG9(Gal)-PA
-
 ?
UDP-alpha-D-galactose + rhamnogalacturonan I
-
 Vigna angularis UDP + 1,4-beta-galactan side chains in rhamnogalacturonan I
-
 ?

Synonyms

Synonyms Comment Organism
RG-GalT
-
 Vigna angularis
RG-I galactosyltransferase
-
 Vigna angularis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Vigna angularis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7
-
 Vigna angularis