Literature summary for 2.4.1.374 extracted from

Sernee, M.; Ralton, J.; Nero, T.; Sobala, L.; Kloehn, J.; Vieira-Lara, M.; Cobbold, S.; Stanton, L.; Pires, D.; Hanssen, E.; Males, A.; Ward, T.; Bastidas, L.; van der Peet, P.; Parker, M.; Ascher, D.; Williams, S.; Davies, G.; McConville, M.
A family of dual-activity glycosyltransferase-phosphorylases mediates mannogen turnover and virulence in Leishmania parasites (2019), Cell Host Microbe, 26, 385-399.e9 .

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Protein Variants

Protein Variants	Comment	Organism
D145N	isoform Mtp2, little effect on transferase activity	Leishmania mexicana
D94N	isoform Mtp2, complete loss of transferase activity	Leishmania mexicana
H161R	isoform Mtp2, reduced transferase activity	Leishmania mexicana

Organism

Organism	UniProt	Comment	Textmining
Leishmania mexicana	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
GDP-alpha-D-mannose + [(1->2)-beta-D-mannosyl]8	-	Leishmania mexicana	GDP + [(1->2)-beta-D-mannosyl]9	-	r
additional information	MTP1 alone produces hyper-elongated mannogen oligomers (DP >60), while expression of MTP2 results in synthesis of a restricted pool of short mannogen oligomers (DP 2-10). Co-expression of both MTP1 and 2 results in an intermediate mannogen profile containing both short and long mannogen oligomers (DP 4 to >60)	Leishmania mexicana	?	-	?

Synonyms

Synonyms	Comment	Organism
mannosyltransferase/phosphorylase	-	Leishmania mexicana
MTP1	-	Leishmania mexicana
MTP2	-	Leishmania mexicana

General Information

General Information	Comment	Organism
physiological function	enzyme catalyzes both the sugar nucleotide-dependent biosynthesis and phosphorolytic turnover of mannogen, glycosyltransferase family 108. Isoforms MTP1 and 2 function primarily as GDPMan-dependent beta-1,2-mannosyltransferases. Parasites lacking isoforms Mtp1-7 do not induce lesions or proliferate in highly susceptible BALB/c mice. Expression of MTP1 alone or MTP1/MTP2 together in the absence of phosphorolytic Mtps exacerbates the loss of virulence phenotype, with no recovery of parasites from tissue biopsies. The mutant lacking Mtp2 does not induce lesions in BALB/c mice even when a high inoculum is used	Leishmania mexicana

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Release 2023.1 (January 2023)