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Literature summary for 2.4.1.37 extracted from

  • Blackler, R.J.; Gagnon, S.M.; Polakowski, R.; Rose, N.L.; Zheng, R.B.; Letts, J.A.; Johal, A.R.; Schuman, B.; Borisova, S.N.; Palcic, M.M.; Evans, S.V.
    Glycosyltransfer in mutants of putative catalytic residue Glu303 of the human ABO(H) A and B blood group glycosyltransferases GTA and GTB proceeds through a labile active site (2017), Glycobiology, 27, 370-380 .
    View publication on PubMed
Search for more literature for 2.4.1.37

Crystallization (Commentary)

Crystallization (Comment) Organism
wild-type and mutants E303A, E303C, E303D, E303Q Homo sapiens

Protein Variants

Protein Variants Comment Organism
E303A residue E303 plays a critical role in maintaining the stability of a strained double-turn in the active site through several hydrogen bonds Homo sapiens
E303C residue E303 plays a critical role in maintaining the stability of a strained double-turn in the active site through several hydrogen bonds. Mutant retains significant activity despite disrupted active site architecture Homo sapiens
E303D residue E303 plays a critical role in maintaining the stability of a strained double-turn in the active site through several hydrogen bonds. Mutant retains significant activity despite disrupted active site architecture Homo sapiens
E303Q residue E303 plays a critical role in maintaining the stability of a strained double-turn in the active site through several hydrogen bonds. Mutant maintains active site architecture but exhibits zero activity Homo sapiens
additional information the enzymes GTA, EC 2.4.1.40, and GTB have nearly identical sequences, while the corresponding mutants of GTA/GTB have up to a 13fold difference in their residual activities relative to wild type.The mutated Cys, Asp and Gln functional groups are no more than 0.8 A further from the anomeric carbon of donor substrate compared to wild type Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.036
-
 UDP-alpha-D-galactose mutant E303C, pH 7.0, 37°C Homo sapiens
0.074
-
 UDP-alpha-D-galactose mutant E303A, pH 7.0, 37°C Homo sapiens
0.19
-
 alpha-Fuc-(1->2)-beta-D-Gal-(CH2)7CH3 mutant E303C, pH 7.0, 37°C Homo sapiens
0.211
-
 UDP-alpha-D-galactose mutant E303D, pH 7.0, 37°C Homo sapiens
0.98
-
 alpha-Fuc-(1->2)-beta-D-Gal-(CH2)7CH3 mutant E303A, pH 7.0, 37°C Homo sapiens
2.64
-
 alpha-Fuc-(1->2)-beta-D-Gal-(CH2)7CH3 mutant E303D, pH 7.0, 37°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P16442
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-alpha-D-galactose + alpha-Fuc-(1->2)-beta-D-Gal-(CH2)7CH3
-
 Homo sapiens UDP + alpha-D-Gal-(1->3)-[alpha-Fuc-(1->2)]-beta-D-Gal-(CH2)7CH3
-
 ?

Synonyms

Synonyms Comment Organism
GTB
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0004
-
 alpha-Fuc-(1->2)-beta-D-Gal-(CH2)7CH3 mutant E303A, pH 7.0, 37°C Homo sapiens
0.0058
-
 alpha-Fuc-(1->2)-beta-D-Gal-(CH2)7CH3 mutant E303D, pH 7.0, 37°C Homo sapiens
1.3
-
 alpha-Fuc-(1->2)-beta-D-Gal-(CH2)7CH3 mutant E303C, pH 7.0, 37°C Homo sapiens