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Literature summary for 2.4.1.37 extracted from

  • Johal, A.; Schuman, B.; Alfaro, J.; Borisova, S.; Seto, N.; Evans, S.
    Sequence-dependent effects of cryoprotectants on the active sites of the human ABO(H) blood group A and B glycosyltransferases (2012), Acta Crystallogr. Sect. D, 68, 268-276.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
structures of GTA, GTB and several chimeras determined by single-crystal X-ray diffraction demonstrate a range of susceptibility to the choice of cryoprotectant, in which the mobile polypeptide loops can be induced by glycerol to form the ordered closed conformation associated with substrate recognition and by MPD (hexylene glycol, 2-methyl-2,4-pentanediol) to hinder binding of substrate in the active site owing to chelation of the Mn2+ cofactor and thereby adopt the disordered open state Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information chimeric GTA and GTB enzymes are generated (AABB, ABBA and ABBB) Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ cofactor Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
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-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information galactose is used as an acceptor analogue and UDP as a donor analogue in all soaking trials Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
blood group B glycosyltransferase
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Homo sapiens
GTB
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Homo sapiens