Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in Escherichia coli | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
wild-type enzyme and GTA/GTB chimeric enzyme mutants GTB/G176R and GTB/G176R/G235S bound to a panel of donor and acceptor analogue substrates, hanging drop vapour diffusion method, method variantions, overview, X-ray diffraction structure determination and analysis at 1.43-1.75 A resolution | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of GTA/GTB chimeric enzyme mutants GTB/G176R and GTB/G176R/G235S, structures of the mutants bound to a panel of donor and acceptor analogue substrates, showing open, semi-closed, and closed conformations as the enzymes go from the unliganded to the liganded states, overview | Homo sapiens |
R188H | site-directed mutagenesis, the mutant shows affected substrate binding | Homo sapiens |
R188K | site-directed mutagenesis, the mutant shows affected substrate binding | Homo sapiens |
R188S | site-directed mutagenesis, the mutant shows affected substrate binding | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell surface | - |
Homo sapiens | 9986 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | - |
Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-galactose + alpha-L-fucosyl-(1,2)-D-galactosyl-R | Homo sapiens | - |
UDP + alpha-D-galactosyl-(1,3)-[alpha-L-fucosyl(1,2)]-D-galactosyl-R | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P16442 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant enzymes from Escherichia coli | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
erythrocyte | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-galactose + alpha-L-fucosyl-(1,2)-D-galactosyl-R | - |
Homo sapiens | UDP + alpha-D-galactosyl-(1,3)-[alpha-L-fucosyl(1,2)]-D-galactosyl-R | - |
? | |
UDP-galactose + alpha-L-fucosyl-(1,2)-D-galactosyl-R | substrate binding structure and residues involved, overview | Homo sapiens | UDP + alpha-D-galactosyl-(1,3)-[alpha-L-fucosyl(1,2)]-D-galactosyl-R | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | high resolution structures for GTB and GTA/GTB chimeric enzyme mutants GTB/G176R and GTB/G176R/G235S bound to a panel of donor and acceptor analogue substrates, showing open, semi-closed, and closed conformations as the enzymes go from the unliganded to the liganded states, the active site in the unliganded or H antigen-bound enzymes is composed of two alpha-helices spanning Arg180-Met186 and Arg188-Asp194, respectively, loop ordering, overview | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
ABO(H) blood group B glycosyltransferase | - |
Homo sapiens |
alpha-(1-3)-galactosyltransferase | - |
Homo sapiens |
GTB | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Homo sapiens |