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Literature summary for 2.4.1.37 extracted from

  • Alfaro, J.A.; Zheng, R.B.; Persson, M.; Letts, J.A.; Polakowski, R.; Bai, Y.; Borisova, S.N.; Seto, N.O.; Lowary, T.L.; Palcic, M.M.; Evans, S.V.
    ABO(H) blood group A and B glycosyltransferases recognize substrate via specific conformational changes (2008), J. Biol. Chem., 283, 10097-10108.
    View publication on PubMed
Search for more literature for 2.4.1.37

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant enzymes in Escherichia coli Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
wild-type enzyme and GTA/GTB chimeric enzyme mutants GTB/G176R and GTB/G176R/G235S bound to a panel of donor and acceptor analogue substrates, hanging drop vapour diffusion method, method variantions, overview, X-ray diffraction structure determination and analysis at 1.43-1.75 A resolution Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information construction of GTA/GTB chimeric enzyme mutants GTB/G176R and GTB/G176R/G235S, structures of the mutants bound to a panel of donor and acceptor analogue substrates, showing open, semi-closed, and closed conformations as the enzymes go from the unliganded to the liganded states, overview Homo sapiens
R188H site-directed mutagenesis, the mutant shows affected substrate binding Homo sapiens
R188K site-directed mutagenesis, the mutant shows affected substrate binding Homo sapiens
R188S site-directed mutagenesis, the mutant shows affected substrate binding Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cell surface
-
 Homo sapiens 9986
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-galactose + alpha-L-fucosyl-(1,2)-D-galactosyl-R Homo sapiens
-
 UDP + alpha-D-galactosyl-(1,3)-[alpha-L-fucosyl(1,2)]-D-galactosyl-R
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens P16442
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type and mutant enzymes from Escherichia coli Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
erythrocyte
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-galactose + alpha-L-fucosyl-(1,2)-D-galactosyl-R
-
 Homo sapiens UDP + alpha-D-galactosyl-(1,3)-[alpha-L-fucosyl(1,2)]-D-galactosyl-R
-
 ?
UDP-galactose + alpha-L-fucosyl-(1,2)-D-galactosyl-R substrate binding structure and residues involved, overview Homo sapiens UDP + alpha-D-galactosyl-(1,3)-[alpha-L-fucosyl(1,2)]-D-galactosyl-R
-
 ?

Subunits

Subunits Comment Organism
More high resolution structures for GTB and GTA/GTB chimeric enzyme mutants GTB/G176R and GTB/G176R/G235S bound to a panel of donor and acceptor analogue substrates, showing open, semi-closed, and closed conformations as the enzymes go from the unliganded to the liganded states, the active site in the unliganded or H antigen-bound enzymes is composed of two alpha-helices spanning Arg180-Met186 and Arg188-Asp194, respectively, loop ordering, overview Homo sapiens

Synonyms

Synonyms Comment Organism
ABO(H) blood group B glycosyltransferase
-
 Homo sapiens
alpha-(1-3)-galactosyltransferase
-
 Homo sapiens
GTB
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Homo sapiens