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Literature summary for 2.4.1.37 extracted from

  • Nakahara, T.; Hindsgaul, O.; Palcic, M.M.; Nishimura, S.
    Computational design and experimental evaluation of glycosyltransferase mutants: engineering of a blood type B galactosyltransferase with enhanced glucosyltransferase activity (2006), Protein Eng. Des. Sel., 19, 571-578.
    View publication on PubMed
Search for more literature for 2.4.1.37

Protein Variants

Protein Variants Comment Organism
S185C mutant enzyme exhibits 4.8fold elevations in kcat/Km for UDP-glucose relative to that of wild-type enzyme Homo sapiens
S185D activity with UDP-galactose is 0.09% of wild-type activity Homo sapiens
S185E activity with UDP-galactose is 0.04% of wild-type activity Homo sapiens
S185N mutant enzyme exhibits 4.3fold elevations in kcat/Km for UDP-glucose relative to that of wild-type enzyme Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.033
-
 UDP-galactose 37°C, pH 7.0, wild-type enzyme S185C Homo sapiens
0.053
-
 UDP-galactose 37°C, pH 7.0, wild-type enzyme Homo sapiens
0.12
-
 UDP-glucose 37°C, pH 7.0, wild-type enzyme S185N Homo sapiens
0.152
-
 UDP-glucose 37°C, pH 7.0, wild-type enzyme S185C Homo sapiens
0.166
-
 UDP-galactose 37°C, pH 7.0, wild-type enzyme S185N Homo sapiens
0.188
-
 UDP-glucose 37°C, pH 7.0, wild-type enzyme Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P16442
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-D-galactose + alpha-L-Fucp-(1,2)-beta-D-Galp-O(CH2)7CH3
-
 Homo sapiens UDP + alpha-L-Fuc-(1,2)-[alpha-D-Galp-(1,3)-]-beta-D-Galp-O(CH2)7CH3
-
 ?
UDP-glucose + Fucalpha1-2Galbeta-O(CH2)7CH3 wild-type enzyme shows very low activity. Mutants, Ser185Asn and Ser185Cys, exhibit 4.3fold and 4.8fold elevation in kcat/Km for UDP-glucose relative to that of wild-type enzyme Homo sapiens ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00053
-
 UDP-glucose 37°C, pH 7.0, wild-type enzyme Homo sapiens
0.0014
-
 UDP-glucose 37°C, pH 7.0, wild-type enzyme S185N Homo sapiens
0.002
-
 UDP-glucose 37°C, pH 7.0, wild-type enzyme S185C Homo sapiens
0.092
-
 UDP-galactose 37°C, pH 7.0, wild-type enzyme S185C Homo sapiens
0.52
-
 UDP-galactose 37°C, pH 7.0, wild-type enzyme S185N Homo sapiens
5.2
-
 UDP-galactose 37°C, pH 7.0, wild-type enzyme Homo sapiens