KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.036 | - |
UDP-galactose | in reaction with 2'-fucosyllactose | Oryctolagus cuniculus | |
0.05 | - |
UDP-galactose | in reaction with N-acetyllactosamine | Oryctolagus cuniculus | |
0.08 | - |
2'-fucosyllactose | - |
Oryctolagus cuniculus | |
0.8 | - |
N-acetyllactosamine | - |
Oryctolagus cuniculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | the enzyme catalyzing the transfer of galactose to 2'-fucosyllactose is much more tightly attached to membrane than the transferase which utilizes N-acetyllactosamine | Oryctolagus cuniculus | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | divalent cation required, Mn2+ most effective, optimum concentration 20 mM | Oryctolagus cuniculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryctolagus cuniculus | - |
2 distinct alpha-3-D-galactosyltransferases: one which is more tightly membrane-bound, resembles the human B-gene-specific transferase in its acceptor specificity, and the second, which is a more soluble enzyme transfers D-galactose to the same positional linkage in unsubstituted beta-D-galactosyl residues | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
stomach | mucosa | Oryctolagus cuniculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | 2 distinct alpha-3-D-galactosyltransferases: one which is more tightly membrane-bound, resembles the human B-gene-specific transferase in its acceptor specificity, and the second, which is a more soluble enzyme transfers D-galactose to the same positional linkage in unsubstituted beta-D-galactosyl residues | Oryctolagus cuniculus | ? | - |
? | |
UDP-galactose + 2'-fucosyllactose | - |
Oryctolagus cuniculus | UDP + alpha-D-galactosyl-1,3-[2'-fucosyllactose] | - |
? | |
UDP-galactose + glycoprotein alpha-L-fucosyl-1,2-D-galactose | - |
Oryctolagus cuniculus | UDP + glycoprotein alpha-D-galactosyl-1,3-[alpha-L-fucosyl-1,2]-D-galactose | - |
? | |
UDP-galactose + N-acetyllactosamine | - |
Oryctolagus cuniculus | UDP + alpha-D-galactosyl-1,3-[N-acetyl-lactosamine] | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.4 | 6.8 | in reaction with N-acetyllactosamine | Oryctolagus cuniculus |
6.8 | - |
in reaction with 2'-fucosyllactose | Oryctolagus cuniculus |