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Literature summary for 2.4.1.361 extracted from

  • Rodrigues, M.; Borges, N.; Almeida, C.; Lamosa, P.; Santos, H.
    A unique beta-1,2-mannosyltransferase of Thermotoga maritima that uses di-myo-inositol phosphate as the mannosyl acceptor (2009), J. Bacteriol., 191, 6105-6115 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.361

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Thermotoga maritima

Inhibitors

Inhibitors Comment Organism Structure
NaCl 400 mM, 49% inhibition Thermotoga maritima

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.7
-
 GDP-alpha-D-mannose pH 7.6, 95°C Thermotoga maritima
16
-
 di-myo-inositol-1,3'-phosphate pH 7.6, 95°C Thermotoga maritima

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required for maximal activity, 10 mM activates 3.3fold Thermotoga maritima

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GDP-alpha-D-mannose + 2-O-beta-D-mannosyl-di-myo-inositol-1,3'-phosphate Thermotoga maritima the enzyme is involved in the synthesis of the solutes 2-O-beta-D-mannosyl-di-myo-inositol-1,3'-phosphate and 2-O-(beta-D-mannosyl-(1->2)-beta-D-mannosyl)-di-myo-inositol-1,3'-phosphate GDP + 2-O-(beta-D-mannosyl-(1->2)-beta-D-mannosyl)-di-myo-inositol-1,3'-phosphate
-
 ?
GDP-alpha-D-mannose + 2-O-beta-D-mannosyl-di-myo-inositol-1,3'-phosphate Thermotoga maritima DSM 3109 the enzyme is involved in the synthesis of the solutes 2-O-beta-D-mannosyl-di-myo-inositol-1,3'-phosphate and 2-O-(beta-D-mannosyl-(1->2)-beta-D-mannosyl)-di-myo-inositol-1,3'-phosphate GDP + 2-O-(beta-D-mannosyl-(1->2)-beta-D-mannosyl)-di-myo-inositol-1,3'-phosphate
-
 ?
GDP-alpha-D-mannose + di-myo-inositol-1,3'-phosphate Thermotoga maritima the enzyme is involved in the synthesis of the solutes 2-O-beta-D-mannosyl-di-myo-inositol-1,3'-phosphate and 2-O-(beta-D-mannosyl-(1->2)-beta-D-mannosyl)-di-myo-inositol-1,3'-phosphate GDP + 2-O-beta-D-mannosyl-di-myo-inositol-1,3'-phosphate
-
 ?
GDP-alpha-D-mannose + di-myo-inositol-1,3'-phosphate Thermotoga maritima DSM 3109 the enzyme is involved in the synthesis of the solutes 2-O-beta-D-mannosyl-di-myo-inositol-1,3'-phosphate and 2-O-(beta-D-mannosyl-(1->2)-beta-D-mannosyl)-di-myo-inositol-1,3'-phosphate GDP + 2-O-beta-D-mannosyl-di-myo-inositol-1,3'-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Thermotoga maritima Q9WYJ4
-
-
Thermotoga maritima DSM 3109 Q9WYJ4
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Thermotoga maritima

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP-alpha-D-mannose + 2-O-beta-D-mannosyl-di-myo-inositol-1,3'-phosphate the enzyme is involved in the synthesis of the solutes 2-O-beta-D-mannosyl-di-myo-inositol-1,3'-phosphate and 2-O-(beta-D-mannosyl-(1->2)-beta-D-mannosyl)-di-myo-inositol-1,3'-phosphate Thermotoga maritima GDP + 2-O-(beta-D-mannosyl-(1->2)-beta-D-mannosyl)-di-myo-inositol-1,3'-phosphate
-
 ?
GDP-alpha-D-mannose + 2-O-beta-D-mannosyl-di-myo-inositol-1,3'-phosphate minor amounts of the tri-mannosylated form are also detected Thermotoga maritima GDP + 2-O-(beta-D-mannosyl-(1->2)-beta-D-mannosyl)-di-myo-inositol-1,3'-phosphate
-
 ?
GDP-alpha-D-mannose + 2-O-beta-D-mannosyl-di-myo-inositol-1,3'-phosphate the enzyme is involved in the synthesis of the solutes 2-O-beta-D-mannosyl-di-myo-inositol-1,3'-phosphate and 2-O-(beta-D-mannosyl-(1->2)-beta-D-mannosyl)-di-myo-inositol-1,3'-phosphate Thermotoga maritima DSM 3109 GDP + 2-O-(beta-D-mannosyl-(1->2)-beta-D-mannosyl)-di-myo-inositol-1,3'-phosphate
-
 ?
GDP-alpha-D-mannose + 2-O-beta-D-mannosyl-di-myo-inositol-1,3'-phosphate minor amounts of the tri-mannosylated form are also detected Thermotoga maritima DSM 3109 GDP + 2-O-(beta-D-mannosyl-(1->2)-beta-D-mannosyl)-di-myo-inositol-1,3'-phosphate
-
 ?
GDP-alpha-D-mannose + di-myo-inositol-1,3'-phosphate the enzyme is involved in the synthesis of the solutes 2-O-beta-D-mannosyl-di-myo-inositol-1,3'-phosphate and 2-O-(beta-D-mannosyl-(1->2)-beta-D-mannosyl)-di-myo-inositol-1,3'-phosphate Thermotoga maritima GDP + 2-O-beta-D-mannosyl-di-myo-inositol-1,3'-phosphate
-
 ?
GDP-alpha-D-mannose + di-myo-inositol-1,3'-phosphate no activity with ADP-glucose, GDP-glucose, UDP-glucose, and TDP-glucose as glycosyl donor Thermotoga maritima GDP + 2-O-beta-D-mannosyl-di-myo-inositol-1,3'-phosphate
-
 ?
GDP-alpha-D-mannose + di-myo-inositol-1,3'-phosphate the enzyme is involved in the synthesis of the solutes 2-O-beta-D-mannosyl-di-myo-inositol-1,3'-phosphate and 2-O-(beta-D-mannosyl-(1->2)-beta-D-mannosyl)-di-myo-inositol-1,3'-phosphate Thermotoga maritima DSM 3109 GDP + 2-O-beta-D-mannosyl-di-myo-inositol-1,3'-phosphate
-
 ?
GDP-alpha-D-mannose + di-myo-inositol-1,3'-phosphate no activity with ADP-glucose, GDP-glucose, UDP-glucose, and TDP-glucose as glycosyl donor Thermotoga maritima DSM 3109 GDP + 2-O-beta-D-mannosyl-di-myo-inositol-1,3'-phosphate
-
 ?

Synonyms

Synonyms Comment Organism
MDIP synthase
-
 Thermotoga maritima
TM_0359
-
 Thermotoga maritima

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
 assay at Thermotoga maritima
95
-
-
 Thermotoga maritima

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
80 100 80°C: about 40% of maximal activity, 100°C: about 65% of maximal activity Thermotoga maritima

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
 assay at Thermotoga maritima

General Information

General Information Comment Organism
metabolism the enzyme is involved in the synthesis of the solutes 2-O-beta-D-mannosyl-di-myo-inositol-1,3'-phosphate and 2-O-(beta-D-mannosyl-(1->2)-beta-D-mannosyl)-di-myo-inositol-1,3'-phosphate. In cells grown under heat stress, 2-O-beta-D-mannosyl-di-myo-inositol-1,3'-phosphate is the major solute, accounting for 43% of the total pool. 2-O-beta-D-Mannosyl-di-myo-inositol-1,3'-phosphate and 2-O-(beta-D-mannosyl-(1->2)-beta-D-mannosyl)-di-myo-inositol-1,3'-phosphate accumulate to similar levels, each corresponding to 11.5% of the total pool Thermotoga maritima