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Literature summary for 2.4.1.360 extracted from

  • Brazier-Hicks, M.; Evans, K.M.; Gershater, M.C.; Puschmann, H.; Steel, P.G.; Edwards, R.
    The C-glycosylation of flavonoids in cereals (2009), J. Biol. Chem., 284, 17926-17934.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
-
Oryza sativa Indica Group

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0025
-
2-hydroxynaringenin pH and temperature not specified in the publication Oryza sativa Indica Group
0.00478
-
phloretin pH and temperature not specified in the publication Oryza sativa Indica Group
0.008
-
2,4,6-trihydroxybenzophenone pH and temperature not specified in the publication Oryza sativa Indica Group
0.0083
-
2',4',6'-trihydroxydihydrochalcone pH and temperature not specified in the publication Oryza sativa Indica Group
0.0165
-
2,5,7-trihydroxyflavanone pH and temperature not specified in the publication Oryza sativa Indica Group

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49430
-
calculated from amino acid sequence Oryza sativa Indica Group
55250
-
calculated from amino acid sequence Oryza sativa Indica Group

Organism

Organism UniProt Comment Textmining
Oryza sativa Indica Group A2YJ67
-
-
Oryza sativa Indica Group C3W7B0
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Oryza sativa Indica Group

Source Tissue

Source Tissue Comment Organism Textmining
seedling
-
Oryza sativa Indica Group
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 UDP-alpha-D-glucose + 2 2,5,7-trihydroxyflavanone meta-stable 2-hydroxyflavanones exist in an equilibirum with their open-form dibenzoylmethane tautomers which probably are the actual glucosyl-acceptors Oryza sativa Indica Group 2 UDP + 6-(beta-D-glucopyranosyl)-2,5,7-trihydroxyflavanone + 8-(beta-D-glucopyranosyl)-2,5,7-trihydroxyflavanone
-
?
2 UDP-alpha-D-glucose + 2 2-hydroxyeriodictyol meta-stable 2-hydroxyflavanones exist in an equilibirum with their open-form dibenzoylmethane tautomers which probably are the actual glucosyl-acceptors Oryza sativa Indica Group 2 UDP + 6-(beta-D-glucopyranosyl)-2-hydroxyeriodictyol + 8-(beta-D-glucopyranosyl)-2-hydroxyeriodictyol
-
?
2 UDP-alpha-D-glucose + 2 2-hydroxynaringenin the meta-stable 2-hydroxynaringenin exists in an equilibirum with its open-form 1-(4-hydroxyphenyl)-3-(2,4,6-trihydroxyphenyl)propane-1,3-dione which is the actual glucosyl-acceptor Oryza sativa Indica Group 2 UDP + 6-(beta-D-glucopyranosyl)-2-hydroxynaringenin + 8-(beta-D-glucopyranosyl)-2-hydroxynaringenin
-
?
UDP-alpha-D-glucose + 2',4',6'-trihydroxydihydrochalcone
-
Oryza sativa Indica Group UDP + ?
-
?
UDP-alpha-D-glucose + 2,4,6-trihydroxybenzophenone
-
Oryza sativa Indica Group UDP + ?
-
?
UDP-alpha-D-glucose + benzyl 2,4,6-trihydroxybenzoate
-
Oryza sativa Indica Group UDP + ?
-
?
UDP-alpha-D-glucose + phloretin
-
Oryza sativa Indica Group UDP + ?
-
?

Synonyms

Synonyms Comment Organism
OsCGT
-
Oryza sativa Indica Group

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.11
-
2,4,6-trihydroxybenzophenone pH and temperature not specified in the publication Oryza sativa Indica Group
0.75
-
2',4',6'-trihydroxydihydrochalcone pH and temperature not specified in the publication Oryza sativa Indica Group
0.76
-
2,5,7-trihydroxyflavanone pH and temperature not specified in the publication Oryza sativa Indica Group
3.13
-
2-hydroxynaringenin pH and temperature not specified in the publication Oryza sativa Indica Group
10.84
-
phloretin pH and temperature not specified in the publication Oryza sativa Indica Group

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
46.2
-
2,5,7-trihydroxyflavanone pH and temperature not specified in the publication Oryza sativa Indica Group
91.2
-
2',4',6'-trihydroxydihydrochalcone pH and temperature not specified in the publication Oryza sativa Indica Group
137
-
2,4,6-trihydroxybenzophenone pH and temperature not specified in the publication Oryza sativa Indica Group
1254
-
2-hydroxynaringenin pH and temperature not specified in the publication Oryza sativa Indica Group
2269
-
3-(4-hydroxyphenyl)-1-(2,4,6-trihydroxyphenyl)propan-1-one pH and temperature not specified in the publication Oryza sativa Indica Group