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Literature summary for 2.4.1.36 extracted from
- The production and utilization of GDP-glucose in the biosynthesis of trehalose 6-phosphate by Streptomyces venezuelae (2017), J. Biol. Chem., 292, 945-954 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli SoluBL21 cells | Streptomyces venezuelae |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.12 | - |
GDP-glucose | pH and temperature not specified in the publication | Streptomyces venezuelae |  |
| 0.44 | - |
ADP-glucose | pH and temperature not specified in the publication | Streptomyces venezuelae | |
| 0.9 | - |
glucose 6-phosphate | with 2.5 mM ADP-glucose as cosubstrate, pH and temperature not specified in the publication | Streptomyces venezuelae | |
| 0.93 | - |
glucose 6-phosphate | with 0.5 mM GDP-glucose as cosubstrate, pH and temperature not specified in the publication | Streptomyces venezuelae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 109000 | - |
gel filtration | Streptomyces venezuelae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GDP-glucose + glucose 6-phosphate | Streptomyces venezuelae | - |
GDP + alpha,alpha-trehalose 6-phosphate | - |
? | |
| GDP-glucose + glucose 6-phosphate | Streptomyces venezuelae ATCC 10712 | - |
GDP + alpha,alpha-trehalose 6-phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces venezuelae | F2RH01 | - |
- |
| Streptomyces venezuelae ATCC 10712 | F2RH01 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| nickel affinity column chromatography and Superdex 200 gel filtration | Streptomyces venezuelae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP-glucose + glucose 6-phosphate | - |
Streptomyces venezuelae | ADP + alpha,alpha-trehalose 6-phosphate | - |
? | |
| ADP-glucose + glucose 6-phosphate | - |
Streptomyces venezuelae ATCC 10712 | ADP + alpha,alpha-trehalose 6-phosphate | - |
? | |
| GDP-glucose + glucose 6-phosphate | - |
Streptomyces venezuelae | GDP + alpha,alpha-trehalose 6-phosphate | - |
? | |
| GDP-glucose + glucose 6-phosphate | the enzyme has a preference for GDP-glucose | Streptomyces venezuelae | GDP + alpha,alpha-trehalose 6-phosphate | - |
? | |
| GDP-glucose + glucose 6-phosphate | - |
Streptomyces venezuelae ATCC 10712 | GDP + alpha,alpha-trehalose 6-phosphate | - |
? | |
| GDP-glucose + glucose 6-phosphate | the enzyme has a preference for GDP-glucose | Streptomyces venezuelae ATCC 10712 | GDP + alpha,alpha-trehalose 6-phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 51000, calculated from amino acid sequence | Streptomyces venezuelae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alpha,alpha-trehalose-phosphate synthase | - |
Streptomyces venezuelae |
| OtsA | - |
Streptomyces venezuelae |
| trehalose-6-phosphate synthase | - |
Streptomyces venezuelae |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 26 | - |
glucose 6-phosphate | with 2.5 mM ADP-glucose as cosubstrate, pH and temperature not specified in the publication | Streptomyces venezuelae | |
| 29 | - |
ADP-glucose | pH and temperature not specified in the publication | Streptomyces venezuelae | |
| 123 | - |
glucose 6-phosphate | with 0.5 mM GDP-glucose as cosubstrate, pH and temperature not specified in the publication | Streptomyces venezuelae | |
| 139 | - |
GDP-glucose | pH and temperature not specified in the publication | Streptomyces venezuelae | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 29 | - |
glucose 6-phosphate | with 2.5 mM ADP-glucose as cosubstrate, pH and temperature not specified in the publication | Streptomyces venezuelae | |
| 67 | - |
ADP-glucose | pH and temperature not specified in the publication | Streptomyces venezuelae | |
| 132 | - |
glucose 6-phosphate | with 0.5 mM GDP-glucose as cosubstrate, pH and temperature not specified in the publication | Streptomyces venezuelae | |
| 1143 | - |
GDP-glucose | pH and temperature not specified in the publication | Streptomyces venezuelae | |
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