BRENDA - Enzyme Database
show all sequences of 2.4.1.359

Exploring the sequence diversity in glycoside hydrolase family 13_18 reveals a novel glucosylglycerol phosphorylase

Franceus, J.; Decuyper, L.; Dhooghe, M.; Desmet, T.; Appl. Microbiol. Biotechnol. 102, 3183-3191 (2018)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expression in Escherichia coli
Marinobacter adhaerens
Crystallization (Commentary)
Crystallization (Commentary)
Organism
homology modeling of structure. Residues Tyr194 and Gln336 each form a hydrogen bond with a hydroxyl group of glycerol, while Ala333 is situated at a suitable location for hydrophobic interaction with atom C1
Marinobacter adhaerens
Engineering
Protein Variants
Commentary
Organism
Q336A
2.6% residual activity
Marinobacter adhaerens
Y194A
2.7% residual activity
Marinobacter adhaerens
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.5
-
alpha-D-glucose 1-phosphate
pH 6.5, 37°C
Marinobacter adhaerens
3.9
-
2-O-(alpha-D-glucopyranosyl)glycerol
pH 6.5, 37°C
Marinobacter adhaerens
7
-
glycerol
pH 6.5, 37°C
Marinobacter adhaerens
10
-
phosphate
pH 6.5, 37°C
Marinobacter adhaerens
Organism
Organism
UniProt
Commentary
Textmining
Marinobacter adhaerens
E4PMA5
member of family GH13_18
-
Marinobacter adhaerens DSM 23420
E4PMA5
member of family GH13_18
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
14.5
-
pH 6.5, 37°C, acceptor substrate 1,2-propanediol
Marinobacter adhaerens
17
-
substrate 2-O-(alpha-D-glucopyranosyl)glycerol, pH 6.5, 37°C
Marinobacter adhaerens
33
-
pH 6.5, 37°C, acceptor substrate glycerol
Marinobacter adhaerens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2-O-(alpha-D-glucopyranosyl)glycerol + phosphate
-
749701
Marinobacter adhaerens
alpha-D-glucose 1-phosphate + glycerol
-
-
-
r
2-O-(alpha-D-glucopyranosyl)glycerol + phosphate
-
749701
Marinobacter adhaerens DSM 23420
alpha-D-glucose 1-phosphate + glycerol
-
-
-
r
alpha-D-glucose 1-phosphate + 1,2-propanediol
-
749701
Marinobacter adhaerens
2-O-(alpha-D-glucopyranosyl)propanediol + phosphate
-
-
-
r
alpha-D-glucose 1-phosphate + 1,2-propanediol
-
749701
Marinobacter adhaerens DSM 23420
2-O-(alpha-D-glucopyranosyl)propanediol + phosphate
-
-
-
r
alpha-D-glucose 1-phosphate + glycerol
-
749701
Marinobacter adhaerens
2-O-(alpha-D-glucopyranosyl)glycerol + phosphate
-
-
-
r
alpha-D-glucose 1-phosphate + glycerol
-
749701
Marinobacter adhaerens DSM 23420
2-O-(alpha-D-glucopyranosyl)glycerol + phosphate
-
-
-
r
additional information
no transglucosylation activity with fructose, fructose 6-phosphate or glycerate as acceptors
749701
Marinobacter adhaerens
?
-
-
-
-
additional information
no transglucosylation activity with fructose, fructose 6-phosphate or glycerate as acceptors
749701
Marinobacter adhaerens DSM 23420
?
-
-
-
-
Synonyms
Synonyms
Commentary
Organism
GGoP
-
Marinobacter adhaerens
GtfA
-
Marinobacter adhaerens
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
-
Marinobacter adhaerens
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
45
-
10 min, no loss of activity
Marinobacter adhaerens
50
-
10 min, 46% residual activity
Marinobacter adhaerens
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3
6
alpha-D-glucose 1-phosphate
pH 6.5, 37°C
Marinobacter adhaerens
14
-
2-O-(alpha-D-glucopyranosyl)glycerol
pH 6.5, 37°C
Marinobacter adhaerens
14
-
phosphate
pH 6.5, 37°C
Marinobacter adhaerens
39
-
glycerol
pH 6.5, 37°C
Marinobacter adhaerens
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
-
Marinobacter adhaerens
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Marinobacter adhaerens
Crystallization (Commentary) (protein specific)
Crystallization
Organism
homology modeling of structure. Residues Tyr194 and Gln336 each form a hydrogen bond with a hydroxyl group of glycerol, while Ala333 is situated at a suitable location for hydrophobic interaction with atom C1
Marinobacter adhaerens
Engineering (protein specific)
Protein Variants
Commentary
Organism
Q336A
2.6% residual activity
Marinobacter adhaerens
Y194A
2.7% residual activity
Marinobacter adhaerens
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.5
-
alpha-D-glucose 1-phosphate
pH 6.5, 37°C
Marinobacter adhaerens
3.9
-
2-O-(alpha-D-glucopyranosyl)glycerol
pH 6.5, 37°C
Marinobacter adhaerens
7
-
glycerol
pH 6.5, 37°C
Marinobacter adhaerens
10
-
phosphate
pH 6.5, 37°C
Marinobacter adhaerens
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
14.5
-
pH 6.5, 37°C, acceptor substrate 1,2-propanediol
Marinobacter adhaerens
17
-
substrate 2-O-(alpha-D-glucopyranosyl)glycerol, pH 6.5, 37°C
Marinobacter adhaerens
33
-
pH 6.5, 37°C, acceptor substrate glycerol
Marinobacter adhaerens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2-O-(alpha-D-glucopyranosyl)glycerol + phosphate
-
749701
Marinobacter adhaerens
alpha-D-glucose 1-phosphate + glycerol
-
-
-
r
2-O-(alpha-D-glucopyranosyl)glycerol + phosphate
-
749701
Marinobacter adhaerens DSM 23420
alpha-D-glucose 1-phosphate + glycerol
-
-
-
r
alpha-D-glucose 1-phosphate + 1,2-propanediol
-
749701
Marinobacter adhaerens
2-O-(alpha-D-glucopyranosyl)propanediol + phosphate
-
-
-
r
alpha-D-glucose 1-phosphate + 1,2-propanediol
-
749701
Marinobacter adhaerens DSM 23420
2-O-(alpha-D-glucopyranosyl)propanediol + phosphate
-
-
-
r
alpha-D-glucose 1-phosphate + glycerol
-
749701
Marinobacter adhaerens
2-O-(alpha-D-glucopyranosyl)glycerol + phosphate
-
-
-
r
alpha-D-glucose 1-phosphate + glycerol
-
749701
Marinobacter adhaerens DSM 23420
2-O-(alpha-D-glucopyranosyl)glycerol + phosphate
-
-
-
r
additional information
no transglucosylation activity with fructose, fructose 6-phosphate or glycerate as acceptors
749701
Marinobacter adhaerens
?
-
-
-
-
additional information
no transglucosylation activity with fructose, fructose 6-phosphate or glycerate as acceptors
749701
Marinobacter adhaerens DSM 23420
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
-
Marinobacter adhaerens
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
45
-
10 min, no loss of activity
Marinobacter adhaerens
50
-
10 min, 46% residual activity
Marinobacter adhaerens
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3
6
alpha-D-glucose 1-phosphate
pH 6.5, 37°C
Marinobacter adhaerens
14
-
2-O-(alpha-D-glucopyranosyl)glycerol
pH 6.5, 37°C
Marinobacter adhaerens
14
-
phosphate
pH 6.5, 37°C
Marinobacter adhaerens
39
-
glycerol
pH 6.5, 37°C
Marinobacter adhaerens
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
-
Marinobacter adhaerens
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
1.4
-
phosphate
pH 6.5, 37°C
Marinobacter adhaerens
3.59
-
2-O-(alpha-D-glucopyranosyl)glycerol
pH 6.5, 37°C
Marinobacter adhaerens
5.57
-
glycerol
pH 6.5, 37°C
Marinobacter adhaerens
14.4
-
alpha-D-glucose 1-phosphate
pH 6.5, 37°C
Marinobacter adhaerens
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
1.4
-
phosphate
pH 6.5, 37°C
Marinobacter adhaerens
3.59
-
2-O-(alpha-D-glucopyranosyl)glycerol
pH 6.5, 37°C
Marinobacter adhaerens
5.57
-
glycerol
pH 6.5, 37°C
Marinobacter adhaerens
14.4
-
alpha-D-glucose 1-phosphate
pH 6.5, 37°C
Marinobacter adhaerens
Other publictions for EC 2.4.1.359
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
749701
Franceus
Exploring the sequence divers ...
Marinobacter adhaerens, Marinobacter adhaerens DSM 23420
Appl. Microbiol. Biotechnol.
102
3183-3191
2018
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