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Literature summary for 2.4.1.357 extracted from
- Genome-wide identification and biochemical characterization of the UGT88F subfamily in Malus x domestica Borkh (2019), Phytochemistry, 157, 135-144 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene UGTF1, located on chromosome 15, DNA and amino acid sequence determination and analysis, quantitative RT-PCR expression analysis | Malus domestica |
| gene UGTF8, located on chromosome 8, DNA and amino acid sequence determination and analysis, quantitative RT-PCR expression analysis, recombinant expression of C-terminally His-tagged isozyme UGT88F8 in Saccharomyces cerevisiae strain W303 | Malus domestica |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00772 | - |
phloretin | pH 7.5, 30°C, recombinant enzyme | Malus domestica |  |
| 0.01084 | - |
UDP-alpha-D-glucose | pH 7.5, 30°C, recombinant enzyme | Malus domestica | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-alpha-D-glucose + phloretin | Malus domestica | - |
UDP + phlorizin | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Malus domestica | B3TKC8 | cv. Golden Delicious | - |
| Malus domestica | S5VDV0 | cv. Golden Delicious | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant C-terminally His-tagged isozyme UGT88F8 from Saccharomyces cerevisiae strain W303 by nickel affinity chromatography and ultrafiltration | Malus domestica |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Malus domestica | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | no activity with several flavonoid classes including chalcones which are structurally related to dihydrochalcones, i.e. eriodictyol chalcone, naringenin, eriodictyol, apigenin, luteolin, kaempferol, quercetin, myricetin, catechin, epicatechin, and cyanidin | Malus domestica | ? | - |
- |
|
| UDP-alpha-D-glucose + phloretin | - |
Malus domestica | UDP + phlorizin | - |
? | |
| UDP-alpha-D-glucose + phloretin | UGT88F8 shows strict specificity of UGT88F8 for phloretin | Malus domestica | UDP + phlorizin | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| UGT88F1 | - |
Malus domestica |
| UGT88F8 | - |
Malus domestica |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Malus domestica |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 8.28 | - |
UDP-alpha-D-glucose | pH 7.5, 30°C, recombinant enzyme | Malus domestica | |
| 41.83 | - |
phloretin | pH 7.5, 30°C, recombinant enzyme | Malus domestica | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Malus domestica |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the enzyme belongs to the UDP-glycosyltransferase UGT88F subfamily, subfamily tree, genomic organization of the UGT88F members on chromosome 15 and 8, overview. UGT88F1 is located closer to UGT88F4 on chromosome 15 and both genes share the same orientation. On chromosome 8, UGT88F6, UGT88F7 and UGT88F8 form a physical cluster sharing the same orientation. The Km values of UGT88F8 are 0.0077 mM and 0.0108 mM for phloretin and UDP-glucose, respectively, and are in the same range as UGT88F1 catalytic parameters thus constituting two isoforms. Co-expression patterns of both UGT88F1 and UGT88F8 argue for a redundant function in phlorizin biosynthesis in planta | Malus domestica |
| evolution | the enzyme belongs to the UDP-glycosyltransferase UGT88F subfamily, subfamily tree, genomic organization of the UGT88F members on chromosome 15 and 8, overview. UGT88F1 is located closer to UGT88F4 on chromosome 15 and both genes share the same orientation. On chromosome 8, UGT88F6, UGT88F7 and UGT88F8 form a physical cluster sharing the same orientation. The Km values of UGT88F8 are 7.72 microM and 10.84 microM for phloretin and UDP-glucose, respectively, and are in the same range as UGT88F1 catalytic parameters thus constituting two isoforms. Coexpression patterns of both UGT88F1 and UGT88F8 argue for a redundant function in phlorizin biosynthesis in planta | Malus domestica |
| additional information | three-dimensional structure analysis, modeling, and structure-function relationship, overview | Malus domestica |
| physiological function | involvement of the enzyme in phlorizin (i.e. phloridzin or phloretin 2'-O-glucoside) synthesis, the main apple tree dihydrochalcone | Malus domestica |
| physiological function | involvement of the enzyme in phlorizin (i.e. phloridzin or phloretin 2'-O-glucoside) synthesis, the main apple tree dihydrochalcone. UGT88F1 is able to efficiently glycosylate dihydrochalcones | Malus domestica |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 763.8 | - |
UDP-alpha-D-glucose | pH 7.5, 30°C, recombinant enzyme | Malus domestica | |
| 5418.4 | - |
phloretin | pH 7.5, 30°C, recombinant enzyme | Malus domestica | |
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