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Literature summary for 2.4.1.352 extracted from

  • Mukherjee, K.; Narindoshvili, T.; Raushel, F.M.
    Discovery of a kojibiose phosphorylase in Escherichia coli K-12 (2018), Biochemistry, 57, 2857-2867 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.352

Cloned(Commentary)

Cloned (Comment) Organism
-
 Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.069
-
 2-O-(alpha-D-glucopyranosyl)-D-glycerate pH 7.5, 30°C Escherichia coli
15.2
-
 phosphate pH 7.5, 30°C Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P76041
-
-
Escherichia coli MG1655 P76041
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-O-(alpha-D-glucopyranosyl)-D-glycerate + phosphate
-
 Escherichia coli alpha-D-glucopyranose 1-phosphate + D-glycerate
-
 r
2-O-(alpha-D-glucopyranosyl)-D-glycerate + phosphate
-
 Escherichia coli MG1655 alpha-D-glucopyranose 1-phosphate + D-glycerate
-
 r
alpha-D-glucopyranose 1-phosphate + D-glycerate less than 1% of this activity is detected in the presence of alpha-D-galactose-1-P, alpha-D-mannose-1-P, or beta-D-glucose-1-P. Less than 5% of this activity is observed with either L-glycerate, D-ribonate, D-allonate, or glycerol Escherichia coli 2-O-(alpha-D-glucopyranosyl)-D-glycerate + phosphate
-
 r
alpha-D-glucopyranose 1-phosphate + D-glycerate less than 1% of this activity is detected in the presence of alpha-D-galactose-1-P, alpha-D-mannose-1-P, or beta-D-glucose-1-P. Less than 5% of this activity is observed with either L-glycerate, D-ribonate, D-allonate, or glycerol Escherichia coli MG1655 2-O-(alpha-D-glucopyranosyl)-D-glycerate + phosphate
-
 r

Synonyms

Synonyms Comment Organism
YcjM
-
 Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1
-
 2-O-(alpha-D-glucopyranosyl)-D-glycerate pH 7.5, 30°C Escherichia coli
2.4
-
 phosphate pH 7.5, 30°C Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.16
-
 phosphate pH 7.5, 30°C Escherichia coli
31
-
 2-O-(alpha-D-glucopyranosyl)-D-glycerate pH 7.5, 30°C Escherichia coli