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Literature summary for 2.4.1.352 extracted from

  • Franceus, J.; Pinel, D.; Desmet, T.
    Glucosylglycerate phosphorylase, an enzyme with novel specificity involved in compatible solute metabolism (2017), Appl. Environ. Microbiol., 83, e01434-17 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Meiothermus silvanus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5
-
phosphate at pH 6.0 and 42°C Meiothermus silvanus
2.6
-
D-glycerate at pH 6.5 and 42°C Meiothermus silvanus
3.5
-
2-O-(alpha-D-glucopyranosyl)-D-glycerate at pH 6.0 and 42°C Meiothermus silvanus
8.1
-
alpha-D-glucopyranose 1-phosphate at pH 6.5 and 42°C Meiothermus silvanus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-O-(alpha-D-glucopyranosyl)-D-glycerate + phosphate Meiothermus silvanus
-
alpha-D-glucopyranose 1-phosphate + D-glycerate
-
r
2-O-(alpha-D-glucopyranosyl)-D-glycerate + phosphate Meiothermus silvanus ATCC 700542
-
alpha-D-glucopyranose 1-phosphate + D-glycerate
-
r
alpha-D-glucopyranose 1-phosphate + D-glycerate Meiothermus silvanus
-
2-O-(alpha-D-glucopyranosyl)-D-glycerate + phosphate
-
r
alpha-D-glucopyranose 1-phosphate + D-glycerate Meiothermus silvanus ATCC 700542
-
2-O-(alpha-D-glucopyranosyl)-D-glycerate + phosphate
-
r

Organism

Organism UniProt Comment Textmining
Meiothermus silvanus D7BAR0
-
-
Meiothermus silvanus ATCC 700542 D7BAR0
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Meiothermus silvanus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-O-(alpha-D-glucopyranosyl)-D-glycerate + phosphate
-
Meiothermus silvanus alpha-D-glucopyranose 1-phosphate + D-glycerate
-
r
2-O-(alpha-D-glucopyranosyl)-D-glycerate + phosphate
-
Meiothermus silvanus ATCC 700542 alpha-D-glucopyranose 1-phosphate + D-glycerate
-
r
alpha-D-glucopyranose 1-phosphate + D-glycerate
-
Meiothermus silvanus 2-O-(alpha-D-glucopyranosyl)-D-glycerate + phosphate
-
r
alpha-D-glucopyranose 1-phosphate + D-glycerate
-
Meiothermus silvanus ATCC 700542 2-O-(alpha-D-glucopyranosyl)-D-glycerate + phosphate
-
r

Synonyms

Synonyms Comment Organism
GGaP
-
Meiothermus silvanus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
42
-
-
Meiothermus silvanus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.83
-
2-O-(alpha-D-glucopyranosyl)-D-glycerate at pH 6.0 and 42°C Meiothermus silvanus
0.98
-
phosphate at pH 6.0 and 42°C Meiothermus silvanus
96
-
alpha-D-glucopyranose 1-phosphate at pH 6.5 and 42°C Meiothermus silvanus
128
-
D-glycerate at pH 6.5 and 42°C Meiothermus silvanus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
optimum pH in phosphorolytic direction Meiothermus silvanus
6.5
-
optimum pH in synthetic direction Meiothermus silvanus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.24
-
2-O-(alpha-D-glucopyranosyl)-D-glycerate at pH 6.0 and 42°C Meiothermus silvanus
0.4
-
phosphate at pH 6.0 and 42°C Meiothermus silvanus
11.8
-
alpha-D-glucopyranose 1-phosphate at pH 6.5 and 42°C Meiothermus silvanus
49.4
-
D-glycerate at pH 6.5 and 42°C Meiothermus silvanus