Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Meiothermus silvanus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.5 | - |
phosphate | at pH 6.0 and 42°C | Meiothermus silvanus | |
2.6 | - |
D-glycerate | at pH 6.5 and 42°C | Meiothermus silvanus | |
3.5 | - |
2-O-(alpha-D-glucopyranosyl)-D-glycerate | at pH 6.0 and 42°C | Meiothermus silvanus | |
8.1 | - |
alpha-D-glucopyranose 1-phosphate | at pH 6.5 and 42°C | Meiothermus silvanus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-O-(alpha-D-glucopyranosyl)-D-glycerate + phosphate | Meiothermus silvanus | - |
alpha-D-glucopyranose 1-phosphate + D-glycerate | - |
r | |
2-O-(alpha-D-glucopyranosyl)-D-glycerate + phosphate | Meiothermus silvanus ATCC 700542 | - |
alpha-D-glucopyranose 1-phosphate + D-glycerate | - |
r | |
alpha-D-glucopyranose 1-phosphate + D-glycerate | Meiothermus silvanus | - |
2-O-(alpha-D-glucopyranosyl)-D-glycerate + phosphate | - |
r | |
alpha-D-glucopyranose 1-phosphate + D-glycerate | Meiothermus silvanus ATCC 700542 | - |
2-O-(alpha-D-glucopyranosyl)-D-glycerate + phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Meiothermus silvanus | D7BAR0 | - |
- |
Meiothermus silvanus ATCC 700542 | D7BAR0 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Meiothermus silvanus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-O-(alpha-D-glucopyranosyl)-D-glycerate + phosphate | - |
Meiothermus silvanus | alpha-D-glucopyranose 1-phosphate + D-glycerate | - |
r | |
2-O-(alpha-D-glucopyranosyl)-D-glycerate + phosphate | - |
Meiothermus silvanus ATCC 700542 | alpha-D-glucopyranose 1-phosphate + D-glycerate | - |
r | |
alpha-D-glucopyranose 1-phosphate + D-glycerate | - |
Meiothermus silvanus | 2-O-(alpha-D-glucopyranosyl)-D-glycerate + phosphate | - |
r | |
alpha-D-glucopyranose 1-phosphate + D-glycerate | - |
Meiothermus silvanus ATCC 700542 | 2-O-(alpha-D-glucopyranosyl)-D-glycerate + phosphate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
GGaP | - |
Meiothermus silvanus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
42 | - |
- |
Meiothermus silvanus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.83 | - |
2-O-(alpha-D-glucopyranosyl)-D-glycerate | at pH 6.0 and 42°C | Meiothermus silvanus | |
0.98 | - |
phosphate | at pH 6.0 and 42°C | Meiothermus silvanus | |
96 | - |
alpha-D-glucopyranose 1-phosphate | at pH 6.5 and 42°C | Meiothermus silvanus | |
128 | - |
D-glycerate | at pH 6.5 and 42°C | Meiothermus silvanus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
optimum pH in phosphorolytic direction | Meiothermus silvanus |
6.5 | - |
optimum pH in synthetic direction | Meiothermus silvanus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.24 | - |
2-O-(alpha-D-glucopyranosyl)-D-glycerate | at pH 6.0 and 42°C | Meiothermus silvanus | |
0.4 | - |
phosphate | at pH 6.0 and 42°C | Meiothermus silvanus | |
11.8 | - |
alpha-D-glucopyranose 1-phosphate | at pH 6.5 and 42°C | Meiothermus silvanus | |
49.4 | - |
D-glycerate | at pH 6.5 and 42°C | Meiothermus silvanus |