Literature summary for 2.4.1.35 extracted from

Lim, E.K.; Higgins, G.S.; Li, Y.; Bowles, D.J.
Regioselectivity of glucosylation of caffeic acid by a UDP-glucose:glucosyltransferase is maintained in planta (2003), Biochem. J., 373, 987-992.

Cloned(Commentary)

Cloned (Comment)	Organism
expressed as a recombinant fusion protein in Escherichia coli. Wide variety of secondary metabolites. Expression of UGT71C1 in transgenic Arabidopsis is driven by the constitutive cauliflower mosaic virus 35 S (CaMV35S) promoter. Nine independent transgenic lines. The level of expression of UGT71C1 is enhanced considerably in several lines, leading to a higher level of the corresponding enzyme activity and a higher level of caffeoyl-3-O-glucoside	Arabidopsis thaliana

Cloned (Comment)

Organism

expressed as a recombinant fusion protein in Escherichia coli. Wide variety of secondary metabolites. Expression of UGT71C1 in transgenic Arabidopsis is driven by the constitutive cauliflower mosaic virus 35 S (CaMV35S) promoter. Nine independent transgenic lines. The level of expression of UGT71C1 is enhanced considerably in several lines, leading to a higher level of the corresponding enzyme activity and a higher level of caffeoyl-3-O-glucoside

Arabidopsis thaliana

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism
0.09	-	quercetin	pH 7.0, 30°C	Arabidopsis thaliana
0.12	-	luteolin	pH 7.0, 30°C	Arabidopsis thaliana
0.32	-	caffeic acid	pH 7.0, 30°C	Arabidopsis thaliana
0.69	-	m-coumaric acid	pH 7.0, 30°C	Arabidopsis thaliana
0.8	-	o-coumaric acid	pH 7.0, 30°C	Arabidopsis thaliana

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
UDP-alpha-D-glucose + quercetin	-	Arabidopsis thaliana	UDP + quercetin 3'-O-beta-D-glucoside + quercetin 7-O-beta-D-glucoside + quercetin 7,3'-di-O-beta-D-glucoside	-	?
UDP-glucose + caffeic acid	specific for the 3-hydroxyl, and not the 4-hydroxyl, position of caffeic acid.	Arabidopsis thaliana	UDP + caffeoyl 3-O-beta-D-glucoside	-	?
UDP-glucose + isoferulic acid	-	Arabidopsis thaliana	UDP + isoferuloyl beta-D-glucoside	-	?
UDP-glucose + luteolin	-	Arabidopsis thaliana	UDP + luteolin 3'-O-beta-D-glucoside + luteolin 7-O-beta-D-glucoside + luteolin 7,3'-di-O-beta-D-glucoside	-	?
UDP-glucose + m-coumaric acid	-	Arabidopsis thaliana	UDP + m-coumaroyl beta-D-glucoside	-	?
UDP-glucose + o-coumaric acid	-	Arabidopsis thaliana	UDP + o-coumaroyl beta-D-glucoside	-	?

Synonyms

Synonyms	Comment	Organism
UDP-glucose:glucosyltransferase	-	Arabidopsis thaliana