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Literature summary for 2.4.1.35 extracted from

  • Taguchi, G.; Yazawa, T.; Hayashida, N.; Okazaki, M.
    Molecular cloning and heterologous expression of novel glucosyltransferases from tobacco cultured cells that have broad substrate specificity and are induced by salicylic acid and auxin (2001), Eur. J. Biochem., 268, 4086-4094.
    View publication on PubMed
Search for more literature for 2.4.1.35

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Nicotiana tabacum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
 kaempferol
-
 Nicotiana tabacum
0.037
-
 UDPglucose
-
 Nicotiana tabacum
0.077
-
 4-Methylumbelliferone
-
 Nicotiana tabacum
0.4
-
 UDP-xylose
-
 Nicotiana tabacum
1.4
-
 scopoletin
-
 Nicotiana tabacum

Organism

Organism UniProt Comment Textmining
Nicotiana tabacum
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.011
-
 UDPxylose as substrate Nicotiana tabacum
0.298
-
 UDPglucose as substrate Nicotiana tabacum
0.354
-
 4-methylumbelliferone as substrate Nicotiana tabacum
0.504
-
 scopoletin as substrate Nicotiana tabacum
1.19
-
 kaempferol as substrate Nicotiana tabacum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-alpha-D-glucose + 3-hydroxyflavone
-
 Nicotiana tabacum UDP + 3-[(beta-D-glucopyranosyl)oxy]-flavone
-
 ?
UDP-glucose + 3-hydroxycoumarine
-
 Nicotiana tabacum UDP + 3-hydroxycoumaryl beta-D-glucoside
-
 ?
UDP-glucose + formononetin
-
 Nicotiana tabacum UDP + formononetin 7-O-beta-D-glucoside
-
 ?
UDP-glucose + isoscopoletin
-
 Nicotiana tabacum UDP + isoscopoletin beta-D-glucoside
-
 ?
UDP-xylose + 4-methylumbelliferone
-
 Nicotiana tabacum UDP + 4-methylumbelliferyl beta-D-xyloside
-
 ?
UDPglucose + 1-naphthol
-
 Nicotiana tabacum UDP + 1-naphthyl beta-D-glucoside
-
 ?
UDPglucose + 2-naphthol
-
 Nicotiana tabacum UDP + 2-naphthyl beta-D-glucoside
-
 ?
UDPglucose + 4'-hydroxyflavone
-
 Nicotiana tabacum UDP + 4'-hydroxyflavone 4'-beta-D-glucoside
-
 ?
UDPglucose + 4-hydroxycoumarine
-
 Nicotiana tabacum UDP + 4-hydroxycoumaryl beta-D-glucoside
-
 ?
UDPglucose + 4-methylumbelliferone
-
 Nicotiana tabacum UDP + 4-methylumbelliferyl beta-D-glucoside
-
 ?
UDPglucose + 5-hydroxyflavone
-
 Nicotiana tabacum UDP + 5-hydroxyflavone 5-beta-D-glucoside
-
 ?
UDPglucose + 6-hydroxyflavone
-
 Nicotiana tabacum UDP + 6-hydroxyflavone 6-beta-D-glucoside
-
 ?
UDPglucose + 7-hydroxyflavone
-
 Nicotiana tabacum UDP + 7-hydroxyflavone 7-beta-D-glucoside
-
 ?
UDPglucose + caffeic acid
-
 Nicotiana tabacum ?
-
 ?
UDPglucose + daphnetin
-
 Nicotiana tabacum ?
-
 ?
UDPglucose + esculetin
-
 Nicotiana tabacum ?
-
 ?
UDPglucose + ferulic acid
-
 Nicotiana tabacum UDP + 4-O-beta-D-glucopyranosyl-3-methoxycinnamic acid
-
 ?
UDPglucose + kaempferol
-
 Nicotiana tabacum ?
-
 ?
UDPglucose + o-hydroxy-trans-cinnamic acid
-
 Nicotiana tabacum UDP + 2-beta-D-glucopyranosyloxy-trans-cinnamic acid
-
 ?
UDPglucose + p-hydroxycinnamic acid
-
 Nicotiana tabacum UDP + 4-beta-D-glucopyranosyloxy-trans-cinnamic acid
-
 ?
UDPglucose + quercetin
-
 Nicotiana tabacum ?
-
 ?
UDPglucose + salicylic acid
-
 Nicotiana tabacum UDP + 2-(beta-D-glucopyranosyloxy)benzoate
-
 ?
UDPglucose + scopoletin UDPxylose also acts as a weak donor Nicotiana tabacum UDP + scopoletin beta-D-glucoside
-
 ?
UDPglucose + umbelliferone
-
 Nicotiana tabacum UDP + umbelliferyl beta-D-glucoside
-
 ?