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Literature summary for 2.4.1.347 extracted from

  • Nobre, A.; Alarico, S.; Fernandes, C.; Empadinhas, N.; da Costa, M.S.
    A unique combination of genetic systems for the synthesis of trehalose in Rubrobacter xylanophilus: properties of a rare actinobacterial TreT (2008), J. Bacteriol., 190, 7939-7946.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.7
-
GDP-glucose 37°C Rubrobacter xylanophilus
2.9
-
D-glucose 6-phosphate 37°C Rubrobacter xylanophilus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ stimulation, maximum activity at 2 mM. In absence of Mg2+, 36% of maximum activity Rubrobacter xylanophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53900
-
x * 53900, calculated Rubrobacter xylanophilus

Organism

Organism UniProt Comment Textmining
Rubrobacter xylanophilus B8R7Q2
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP-alpha-D-glucose + D-glucose 6-phosphate GDP-glucose is the preferred glycosyl-donor Rubrobacter xylanophilus GDP + alpha,alpha-trehalose 6-phosphate
-
?

Subunits

Subunits Comment Organism
? x * 53900, calculated Rubrobacter xylanophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Rubrobacter xylanophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
half-life 1.7 h Rubrobacter xylanophilus
70
-
half-life 0.7 h Rubrobacter xylanophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Rubrobacter xylanophilus