Literature summary for 2.4.1.345 extracted from

Giganti, D.; Alegre-Cebollada, J.; Urresti, S.; Albesa-Jove, D.; Rodrigo-Unzueta, A.; Comino, N.; Kachala, M.; Lopez-Fernandez, S.; Svergun, D.I.; Fernandez, J.M.; Guerin, M.E.
Conformational plasticity of the essential membrane-associated mannosyltransferase PimA from mycobacteria (2013), J. Biol. Chem., 288, 29797-29808.

Search for more literature for 2.4.1.345

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL3 cells	Mycolicibacterium smegmatis
expression in Escherichia coli	Mycolicibacterium smegmatis

Protein Variants

Protein Variants	Comment	Organism
additional information	in a polyprotein containing PimA flanked by four copies of the I27 protein, which provides a mechanical fingerprint, PimA exhibits weak mechanical stability albeit displaying beta-sheet topology expected to unfold at much higher forces. PimA unfolds following heterogeneous multiple step mechanical unfolding pathways at low force akin to molten globule states. The open and closed conformations of the GT-B glycosyltransferase are largely present in solution, and in addition, PimA experiences remarkable flexibility that corresponds to the N-terminal Rossmann fold domain	Mycolicibacterium smegmatis

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	-	Mycolicibacterium smegmatis	16020	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
GDP-mannose + 1-phosphatidyl-myo-inositol	Mycolicibacterium smegmatis	-	?	-	?
GDP-mannose + 1-phosphatidyl-myo-inositol	Mycolicibacterium smegmatis ATCC 700084	-	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Mycolicibacterium smegmatis	A0QWG6	-	-
Mycolicibacterium smegmatis ATCC 700084	A0QWG6	-	-

Purification (Commentary)

Purification (Comment)	Organism
Talon resin column chromatpgraphy and Superdex 200 gel filtration	Mycolicibacterium smegmatis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
GDP-mannose + 1-phosphatidyl-myo-inositol	-	Mycolicibacterium smegmatis	?	-	?
GDP-mannose + 1-phosphatidyl-myo-inositol	-	Mycolicibacterium smegmatis ATCC 700084	?	-	?

Synonyms

Synonyms	Comment	Organism
MSMEG_2935	-	Mycolicibacterium smegmatis
phosphatidyl-myo-inositol mannosyltransferase A	-	Mycolicibacterium smegmatis
PimA	-	Mycolicibacterium smegmatis

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)