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Literature summary for 2.4.1.345 extracted from
- Expression and purification of a functionally active recombinant GDP-mannosyltransferase (PimA) from Mycobacterium tuberculosis H37Rv (2005), Protein Expr. Purif., 42, 47-53.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Mycobacterium tuberculosis |
| the pimA gene is cloned into the pET82a vector and the recombinant plasmid is transformed into Escherichia coli BL21 (DE3) | Mycobacterium tuberculosis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.018 | - |
GDP-alpha-D-mannose | pH 7.0, 37° | Mycobacterium tuberculosis |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required, optimal concentration is 2-3 mM | Mycobacterium tuberculosis | |
| Mg2+ | about 90% increase of activtiy in presence of 2-3 mM Mg2+ | Mycobacterium tuberculosis | |
| Mn2+ | may partly substitute for Mg2+ | Mycobacterium tuberculosis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 42000 | - |
- |
Mycobacterium tuberculosis |
| 42000 | - |
x * 42000, SDS-PAGE | Mycobacterium tuberculosis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GDP-alpha-D-mannose + 1-phosphatidyl-1D-myo-inositol | Mycobacterium tuberculosis | - |
GDP + 2-O-(alpha-D-mannosyl)-1-phosphatidyl-1D-myo-inositol | - |
? | |
| GDP-alpha-D-mannose + 1-phosphatidyl-1D-myo-inositol | Mycobacterium tuberculosis H37Rv | - |
GDP + 2-O-(alpha-D-mannosyl)-1-phosphatidyl-1D-myo-inositol | - |
? | |
| GDP-mannose + phosphatidyl-myo-inositol | Mycobacterium tuberculosis | PimA plays an essential role in the growth of mycobacteria | GDP + phosphatidyl-(2-O-alpha-D-manno-pyranosyl)-myo-inositol | - |
? | |
| GDP-mannose + phosphatidyl-myo-inositol | Mycobacterium tuberculosis H37Rv | PimA plays an essential role in the growth of mycobacteria | GDP + phosphatidyl-(2-O-alpha-D-manno-pyranosyl)-myo-inositol | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycobacterium tuberculosis | - |
H37Rv | - |
| Mycobacterium tuberculosis | P9WMZ5 | - |
- |
| Mycobacterium tuberculosis H37Rv | - |
H37Rv | - |
| Mycobacterium tuberculosis H37Rv | P9WMZ5 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Mycobacterium tuberculosis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Mycobacterium tuberculosis |
| 9.4 | - |
pH 7.0, 37°C | Mycobacterium tuberculosis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GDP-alpha-D-mannose + 1-phosphatidyl-1D-myo-inositol | - |
Mycobacterium tuberculosis | GDP + 2-O-(alpha-D-mannosyl)-1-phosphatidyl-1D-myo-inositol | - |
? | |
| GDP-alpha-D-mannose + 1-phosphatidyl-1D-myo-inositol | - |
Mycobacterium tuberculosis H37Rv | GDP + 2-O-(alpha-D-mannosyl)-1-phosphatidyl-1D-myo-inositol | - |
? | |
| GDP-alpha-D-mannose + 1-phosphatidyl-myo-inositol | substrate bovine phosphatidylinositol | Mycobacterium tuberculosis | GDP + 2-O-(alpha-D-mannosyl)-1-phosphatidyl-1D-myo-inositol | - |
? | |
| GDP-alpha-D-mannose + 1-phosphatidyl-myo-inositol | substrate bovine phosphatidylinositol | Mycobacterium tuberculosis H37Rv | GDP + 2-O-(alpha-D-mannosyl)-1-phosphatidyl-1D-myo-inositol | - |
? | |
| GDP-mannose + phosphatidyl-myo-inositol | - |
Mycobacterium tuberculosis | GDP + phosphatidyl-(2-O-alpha-D-manno-pyranosyl)-myo-inositol | - |
? | |
| GDP-mannose + phosphatidyl-myo-inositol | PimA plays an essential role in the growth of mycobacteria | Mycobacterium tuberculosis | GDP + phosphatidyl-(2-O-alpha-D-manno-pyranosyl)-myo-inositol | - |
? | |
| GDP-mannose + phosphatidyl-myo-inositol | - |
Mycobacterium tuberculosis H37Rv | GDP + phosphatidyl-(2-O-alpha-D-manno-pyranosyl)-myo-inositol | - |
? | |
| GDP-mannose + phosphatidyl-myo-inositol | PimA plays an essential role in the growth of mycobacteria | Mycobacterium tuberculosis H37Rv | GDP + phosphatidyl-(2-O-alpha-D-manno-pyranosyl)-myo-inositol | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 42000, SDS-PAGE | Mycobacterium tuberculosis |
| ? | x * 42605, MALDI-TOF, x * 42739, calculated | Mycobacterium tuberculosis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GDP-mannosyltransferase | - |
Mycobacterium tuberculosis |
| PimA | - |
Mycobacterium tuberculosis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Mycobacterium tuberculosis |
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