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Literature summary for 2.4.1.344 extracted from

  • Masutani, H.; Kimura, H.
    Purification and characterization of secretory-type GDP-L-fucose:beta-D-galactoside 2-alpha-L-fucosyltransferase from human gastric mucosa (1995), J. Biochem., 118, 541-545.
    View publication on PubMed
Search for more literature for 2.4.1.344

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.095
-
 GDP-beta-L-fucose pH 7.2, temperature not specified in the publication Homo sapiens
1.1
-
 beta-D-galactosyl-(1->4)-N-acetyl-D-glucosamine pH 7.2, temperature not specified in the publication Homo sapiens
13
-
 phenyl beta-D-galactopyranoside pH 7.2, temperature not specified in the publication Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ 10 mM, 6% of the activity with Mn2+ Homo sapiens
Ca2+ 10 mM, 45% of the activity with Mn2+ Homo sapiens
Co2+ 10 mM, 48% of the activity with Mn2+ Homo sapiens
Mg2+ 10 mM, 65% of the activity with Mn2+ Homo sapiens
Mn2+ 10 mM, best activator Homo sapiens
additional information divalent cation is absolutely required, no activation with Zn2+ Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
 x * 65000, SDS-PAGE Homo sapiens
320000
-
 gel filtration Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
mucosa gastric mucosa Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP-beta-L-fucose + beta-D-galactosyl-(1->3)-N-acetyl-D-glucosamine i.e. lacto-N-biose I Homo sapiens GDP + alpha-L-fucosyl-(1->2)-beta-D-galactosyl-(1->3)-N-acetyl-D-glucosamine reaction of EC 2.4.1.69 ?
GDP-beta-L-fucose + beta-D-galactosyl-(1->4)-N-acetyl-D-glucosamine i.e. N-acetyllactosamine Homo sapiens GDP + alpha-L-fucosyl-(1->2)-beta-D-galactosyl-(1->4)-N-acetyl-D-glucosamine
-
 ?
GDP-beta-L-fucose + phenyl beta-D-galactopyranoside
-
 Homo sapiens GDP + phenyl alpha-L-fucosyl-(1->2)-beta-D-galactopyranoside
-
 ?
additional information enzyme accepts both precursors of type 1 and type 2 H epitopes, catalyzing reactions of EC 2.4.1.69 and EC 2.4.1.344. The enzyme does not display alpha-(1,3)-fucosyltransferase activity or alpha-(1, 4)-fucosyl transferase activity Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
multimer x * 65000, SDS-PAGE Homo sapiens