KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.095 | - |
GDP-beta-L-fucose | pH 7.2, temperature not specified in the publication | Homo sapiens | |
1.1 | - |
beta-D-galactosyl-(1->4)-N-acetyl-D-glucosamine | pH 7.2, temperature not specified in the publication | Homo sapiens | |
13 | - |
phenyl beta-D-galactopyranoside | pH 7.2, temperature not specified in the publication | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | 10 mM, 6% of the activity with Mn2+ | Homo sapiens | |
Ca2+ | 10 mM, 45% of the activity with Mn2+ | Homo sapiens | |
Co2+ | 10 mM, 48% of the activity with Mn2+ | Homo sapiens | |
Mg2+ | 10 mM, 65% of the activity with Mn2+ | Homo sapiens | |
Mn2+ | 10 mM, best activator | Homo sapiens | |
additional information | divalent cation is absolutely required, no activation with Zn2+ | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
65000 | - |
x * 65000, SDS-PAGE | Homo sapiens |
320000 | - |
gel filtration | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mucosa | gastric mucosa | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-beta-L-fucose + beta-D-galactosyl-(1->3)-N-acetyl-D-glucosamine | i.e. lacto-N-biose I | Homo sapiens | GDP + alpha-L-fucosyl-(1->2)-beta-D-galactosyl-(1->3)-N-acetyl-D-glucosamine | reaction of EC 2.4.1.69 | ? | |
GDP-beta-L-fucose + beta-D-galactosyl-(1->4)-N-acetyl-D-glucosamine | i.e. N-acetyllactosamine | Homo sapiens | GDP + alpha-L-fucosyl-(1->2)-beta-D-galactosyl-(1->4)-N-acetyl-D-glucosamine | - |
? | |
GDP-beta-L-fucose + phenyl beta-D-galactopyranoside | - |
Homo sapiens | GDP + phenyl alpha-L-fucosyl-(1->2)-beta-D-galactopyranoside | - |
? | |
additional information | enzyme accepts both precursors of type 1 and type 2 H epitopes, catalyzing reactions of EC 2.4.1.69 and EC 2.4.1.344. The enzyme does not display alpha-(1,3)-fucosyltransferase activity or alpha-(1, 4)-fucosyl transferase activity | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
multimer | x * 65000, SDS-PAGE | Homo sapiens |