Literature summary for 2.4.1.34 extracted from

Hrmova, M.; Stone, B.A.; Fincher, G.B.
High-yield production, refolding and a molecular modelling of the catalytic module of (1,3)-beta-D-glucan (curdlan) synthase from Agrobacterium sp (2010), Glycoconj. J., 27, 461-476.

Cloned(Commentary)

Cloned (Comment)	Organism
the catalytic module of CrdS (cm-CrdS) is expressed in good yield from a cDNA encoding cm-CrdS cloned into a vector, containing a coding region for thioredoxin, and from the Champion pET SUMO system that possesses a coding region of a small ubiquitin-related modifier (SUMO) partner protein. The two DNA fusions are expressed as chimeric proteins. High yields of inclusion bodies are produced in Escherichia coli and these could be refolded to form soluble proteins, using a range of buffers and non-detergent sulfobetaines	Agrobacterium sp.

Cloned (Comment)

Organism

the catalytic module of CrdS (cm-CrdS) is expressed in good yield from a cDNA encoding cm-CrdS cloned into a vector, containing a coding region for thioredoxin, and from the Champion pET SUMO system that possesses a coding region of a small ubiquitin-related modifier (SUMO) partner protein. The two DNA fusions are expressed as chimeric proteins. High yields of inclusion bodies are produced in Escherichia coli and these could be refolded to form soluble proteins, using a range of buffers and non-detergent sulfobetaines

Agrobacterium sp.

Organism

Organism	UniProt	Comment	Textmining
Agrobacterium sp.	-	-	-

Synonyms

Synonyms	Comment	Organism
(1,3)-beta-D-glucan (curdlan) synthase	-	Agrobacterium sp.

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)