Activating Compound | Comment | Organism | Structure |
---|---|---|---|
CHAPS | pollen CalS isozyme is activated in vitro by the detergent CHAPS, but activation is not associated with a detectable change in the molecular mass of the NaGSL1 polypeptide | Nicotiana alata | |
additional information | the somatic isozyme is activated by wounding | Nicotiana alata | |
Trypsin | pollen CalS isozyme, in contrast to the somatic isozyme, is activated in vitro by the proteolytic enzyme trypsin, but activation is not associated with a detectable change in the molecular mass of the NaGSL1 polypeptide. Some CalS activity is lost with longer incubations with trypsin | Nicotiana alata |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | the somatic isozyme is inactivated by trypsin, in contrast to the pollen isozyme | Nicotiana alata |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum | inactive, latent enzyme form | Nicotiana alata | 5783 | - |
Golgi membrane | inactive, latent enzyme form | Nicotiana alata | 139 | - |
additional information | predominantly in the endoplasmic reticulum and Golgi membranes in younger pollen tubes mostly in an inactive, latent enzyme form, the latent and active enzyme forms together In later stages of pollen-tube growth to a greater proportion located in intracellular vesicles and the plasma membrane | Nicotiana alata | - |
- |
plasma membrane | inactive, latent enzyme form and highly active enzyme form, Ca2+-independent developmentally regulated CalS | Nicotiana alata | 5886 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
x * 28000, pollen isozyme, SDS-PAGE | Nicotiana alata |
220000 | - |
pollen isozyme | Nicotiana alata |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nicotiana alata | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | Nicotiana alata contains two CalS isozymes, one is the Ca2+-independent, trypsin activable pollen-tube CalS, the other is the wound-activated somatic cell CalS, which is Ca2+-dependent and generally inactivated by trypsin | Nicotiana alata | - |
pollen | pollen isozyme: predominantly in the endoplasmic reticulum and Golgi membranes in younger pollen tubes mostly in an inactive, latent enzyme form, the latent and active enzyme forms together In later stages of pollen-tube growth to a greater proportion located in intracellular vesicles and the plasma membrane. No activity in mature pollen | Nicotiana alata | - |
somatic cell | somatic isozyme | Nicotiana alata | - |
Subunits | Comment | Organism |
---|---|---|
polymer | x * 28000, pollen isozyme, SDS-PAGE | Nicotiana alata |
Synonyms | Comment | Organism |
---|---|---|
callose synthase | - |
Nicotiana alata |
CalS | - |
Nicotiana alata |
glucan synthase-like 1 | - |
Nicotiana alata |
GSL1 | - |
Nicotiana alata |
uridine-diphosphate glucose:1,3-beta-D-glucosyltransferase | - |
Nicotiana alata |
General Information | Comment | Organism |
---|---|---|
physiological function | the active enzyme in the plasma membrane is reponsible for callose synthesis | Nicotiana alata |