BRENDA - Enzyme Database
show all sequences of 2.4.1.337

High-yield expression and purification of a monotopic membrane glycosyltransferase

Eriksson, H.M.; Persson, K.; Zhang, S.; Wieslander, K.; Protein Expr. Purif. 66, 143-148 (2009)

Data extracted from this reference:

Application
Application
Commentary
Organism
synthesis
optimization of protein expression through controlling a few basic expression parameters, including temperature and growth media. The final expression level can be increased by two orders of magnitude, reaching 170 mg of pure protein per litre culture
Acholeplasma laidlawii
Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Acholeplasma laidlawii
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Acholeplasma laidlawii
Q93P60
-
-
Purification (Commentary)
Commentary
Organism
during purification, the concentration of detergent is just as important as the type of detergent, and a low concentration of n-dodecyl-beta-D-maltoside (about 1 x critical micelle concentration) is the best for keeping the protein stable and homogeneous
Acholeplasma laidlawii
Application (protein specific)
Application
Commentary
Organism
synthesis
optimization of protein expression through controlling a few basic expression parameters, including temperature and growth media. The final expression level can be increased by two orders of magnitude, reaching 170 mg of pure protein per litre culture
Acholeplasma laidlawii
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Acholeplasma laidlawii
Purification (Commentary) (protein specific)
Commentary
Organism
during purification, the concentration of detergent is just as important as the type of detergent, and a low concentration of n-dodecyl-beta-D-maltoside (about 1 x critical micelle concentration) is the best for keeping the protein stable and homogeneous
Acholeplasma laidlawii
Other publictions for EC 2.4.1.337
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
734257
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289
10104-10114
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735211
Eriksson
High-yield expression and puri ...
Acholeplasma laidlawii
Protein Expr. Purif.
66
143-148
2009
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658012
Li
Irreversible binding and activ ...
Acholeplasma laidlawii
Biochemistry
42
9677-9686
2003
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639549
Berg
Sequence properties of the 1,2 ...
Acholeplasma laidlawii
J. Biol. Chem.
276
22056-22063
2001
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639548
Li
Activating amphiphiles cause a ...
Acholeplasma laidlawii
J. Biol. Chem.
272
29602-29606
1997
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639550
Karlsson
Lipid dependence and basic kin ...
Acholeplasma laidlawii
J. Biol. Chem.
272
929-936
1997
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