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Literature summary for 2.4.1.335 extracted from

  • Larkin, A.; Chang, M.M.; Whitworth, G.E.; Imperiali, B.
    Biochemical evidence for an alternate pathway in N-linked glycoprotein biosynthesis (2013), Nat. Chem. Biol., 9, 367-373.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.335

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli with N-terminal T7 and C-terminal His6 tags Methanococcus voltae

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane the enzyme is purified from membrane fraction of recombinant Escherichia coli Methanococcus voltae 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37328
-
 x * 37328, calculated from sequence Methanococcus voltae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-2,3-diacetamido-2,3-dideoxy-alpha-D-glucuronate + dolichyl N-acetyl-alpha-D-glucosaminyl phosphate Methanococcus voltae the enzyme participates in the N-linked glycosylation in Methanococcus voltae. Methanococcus voltae generates N-linked glycoproteins with a unique trisaccharide composed of ManNAc(6Thr)A-beta1,4-Glc-2,3-diNAcA-beta1,3-GlcNAc in which threonine is linked via an amide to the terminal mannuronic acid UDP + dolichyl 3-O-(2,3-diacetamido-2,3-dideoxy-beta-D-glucuronsyl)-N-acetyl-alpha-D-glucosaminyl phosphate
-
 ?
UDP-2,3-diacetamido-2,3-dideoxy-alpha-D-glucuronate + dolichyl N-acetyl-alpha-D-glucosaminyl phosphate Methanococcus voltae PS the enzyme participates in the N-linked glycosylation in Methanococcus voltae. Methanococcus voltae generates N-linked glycoproteins with a unique trisaccharide composed of ManNAc(6Thr)A-beta1,4-Glc-2,3-diNAcA-beta1,3-GlcNAc in which threonine is linked via an amide to the terminal mannuronic acid UDP + dolichyl 3-O-(2,3-diacetamido-2,3-dideoxy-beta-D-glucuronsyl)-N-acetyl-alpha-D-glucosaminyl phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Methanococcus voltae B3VA59
-
-
Methanococcus voltae PS B3VA59
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Methanococcus voltae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-2,3-diacetamido-2,3-dideoxy-alpha-D-glucuronate + dolichyl N-acetyl-alpha-D-glucosaminyl phosphate the enzyme participates in the N-linked glycosylation in Methanococcus voltae. Methanococcus voltae generates N-linked glycoproteins with a unique trisaccharide composed of ManNAc(6Thr)A-beta1,4-Glc-2,3-diNAcA-beta1,3-GlcNAc in which threonine is linked via an amide to the terminal mannuronic acid Methanococcus voltae UDP + dolichyl 3-O-(2,3-diacetamido-2,3-dideoxy-beta-D-glucuronsyl)-N-acetyl-alpha-D-glucosaminyl phosphate
-
 ?
UDP-2,3-diacetamido-2,3-dideoxy-alpha-D-glucuronate + dolichyl N-acetyl-alpha-D-glucosaminyl phosphate the enzyme is specific for dolichyl N-acetyl-alpha-D-glucosaminyl phosphate, as no conversion is observed with Dol-P or Dol-PP-GlcNAc. No activity with UDP-Glc, UDP-GalNAc or UDP-Gal. Although the full length N-glycan generated by Methanococcus voltae is a trisaccharide (composed of ManNAc(6Thr)A-beta1,4-Glc-2,3-diNAcA-beta1,3-GlcNAc in which threonine is linked via an amide to the terminal mannuronic acid) the disaccharide-linked Methanococcus voltae UDP + dolichyl 3-O-(2,3-diacetamido-2,3-dideoxy-beta-D-glucuronsyl)-N-acetyl-alpha-D-glucosaminyl phosphate
-
 ?
UDP-2,3-diacetamido-2,3-dideoxy-alpha-D-glucuronate + dolichyl N-acetyl-alpha-D-glucosaminyl phosphate the enzyme participates in the N-linked glycosylation in Methanococcus voltae. Methanococcus voltae generates N-linked glycoproteins with a unique trisaccharide composed of ManNAc(6Thr)A-beta1,4-Glc-2,3-diNAcA-beta1,3-GlcNAc in which threonine is linked via an amide to the terminal mannuronic acid Methanococcus voltae PS UDP + dolichyl 3-O-(2,3-diacetamido-2,3-dideoxy-beta-D-glucuronsyl)-N-acetyl-alpha-D-glucosaminyl phosphate
-
 ?
UDP-2,3-diacetamido-2,3-dideoxy-alpha-D-glucuronate + dolichyl N-acetyl-alpha-D-glucosaminyl phosphate the enzyme is specific for dolichyl N-acetyl-alpha-D-glucosaminyl phosphate, as no conversion is observed with Dol-P or Dol-PP-GlcNAc. No activity with UDP-Glc, UDP-GalNAc or UDP-Gal. Although the full length N-glycan generated by Methanococcus voltae is a trisaccharide (composed of ManNAc(6Thr)A-beta1,4-Glc-2,3-diNAcA-beta1,3-GlcNAc in which threonine is linked via an amide to the terminal mannuronic acid) the disaccharide-linked Methanococcus voltae PS UDP + dolichyl 3-O-(2,3-diacetamido-2,3-dideoxy-beta-D-glucuronsyl)-N-acetyl-alpha-D-glucosaminyl phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 37328, calculated from sequence Methanococcus voltae

Synonyms

Synonyms Comment Organism
AglC
-
 Methanococcus voltae
Mv990 locus name Methanococcus voltae
UDP-Glc-2,3-diNAcA glycosyltransferase
-
 Methanococcus voltae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Methanococcus voltae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Methanococcus voltae

General Information

General Information Comment Organism
metabolism the enzyme participates in the N-linked glycosylation in Methanococcus voltae. Methanococcus voltae generates N-linked glycoproteins with a unique trisaccharide composed of ManNAc(6Thr)A-beta1,4-Glc-2,3-diNAcA-beta1,3-GlcNAc in which threonine is linked via an amide to the terminal mannuronic acid Methanococcus voltae