Literature summary for 2.4.1.333 extracted from

Nakajima, M.; Tanaka, N.; Furukawa, N.; Nihira, T.; Kodutsumi, Y.; Takahashi, Y.; Sugimoto, N.; Miyanaga, A.; Fushinobu, S.; Taguchi, H.; Nakai, H.
Mechanistic insight into the substrate specificity of 1,2-beta-oligoglucan phosphorylase from Lachnoclostridium phytofermentans (2017), Sci. Rep., 7, 42671 .

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Crystallization (Commentary)

Crystallization (Comment)	Organism
the tertiary structure consists of two N-terminal beta-sandwich domains, a catalytic (alpha/alpha)6 barrel domain, and a beta sheet domain. Sophorose binds at subsites +1 to +2. The Glc moiety at subsite +1 is flipped compared with the corresponding ligands in other GH94 enzymes	Lachnoclostridium phytofermentans

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.8	-	2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose	phosphorolysis, pH 7, 40°C	Lachnoclostridium phytofermentans
2.2	-	2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose	synthesis, pH 7, 40°C	Lachnoclostridium phytofermentans
2.5	-	2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose	phosphorolysis, pH 7, 40°C	Lachnoclostridium phytofermentans
2.6	-	alpha-D-glucose 1-phosphate	pH 7, 40°C	Lachnoclostridium phytofermentans
2.8	-	2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose	synthesis, pH 7, 40°C	Lachnoclostridium phytofermentans
4.8	-	2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose	phosphorolysis, pH 7, 40°C	Lachnoclostridium phytofermentans
5.5	-	2-O-beta-D-glucopyranosyl-D-glucopyranose	synthesis, pH 7, 40°C	Lachnoclostridium phytofermentans
11	-	phosphate	pH 7, 40°C	Lachnoclostridium phytofermentans

Organism

Organism	UniProt	Comment	Textmining
Lachnoclostridium phytofermentans	A9KJS6	-	-
Lachnoclostridium phytofermentans DSM 18823	A9KJS6	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose + phosphate	-	Lachnoclostridium phytofermentans	2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose + alpha-D-glucose 1-phosphate	-	?
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose + phosphate	-	Lachnoclostridium phytofermentans DSM 18823	2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose + alpha-D-glucose 1-phosphate	-	?
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose + alpha-D-glucose 1-phosphate	-	Lachnoclostridium phytofermentans	2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose + phosphate	-	?
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose + alpha-D-glucose 1-phosphate	-	Lachnoclostridium phytofermentans DSM 18823	2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose + phosphate	-	?
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose + phosphate	-	Lachnoclostridium phytofermentans	2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose + alpha-D-glucose 1-phosphate	-	?
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose + phosphate	-	Lachnoclostridium phytofermentans DSM 18823	2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose + alpha-D-glucose 1-phosphate	-	?
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose + alpha-D-glucose 1-phosphate	-	Lachnoclostridium phytofermentans	2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose + phosphate	-	?
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose + alpha-D-glucose 1-phosphate	-	Lachnoclostridium phytofermentans DSM 18823	2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose + phosphate	-	?
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose + phosphate	-	Lachnoclostridium phytofermentans	2-O-beta-D-glucopyranosyl-D-glucopyranose + alpha-D-glucose 1-phosphate	-	?
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose + phosphate	-	Lachnoclostridium phytofermentans DSM 18823	2-O-beta-D-glucopyranosyl-D-glucopyranose + alpha-D-glucose 1-phosphate	-	?
2-O-beta-D-glucopyranosyl-D-glucopyranose + alpha-D-glucose 1-phosphate	-	Lachnoclostridium phytofermentans	2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose + phosphate	-	?

Subunits

Subunits	Comment	Organism
monomer	crystallization data	Lachnoclostridium phytofermentans

Synonyms

Synonyms	Comment	Organism
Cphy_0694	-	Lachnoclostridium phytofermentans
SOGP	-	Lachnoclostridium phytofermentans

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
40	-	-	Lachnoclostridium phytofermentans

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
37	-	stable up to	Lachnoclostridium phytofermentans

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
2	8	alpha-D-glucose 1-phosphate	pH 7, 40°C	Lachnoclostridium phytofermentans
40	-	2-O-beta-D-glucopyranosyl-D-glucopyranose	synthesis, pH 7, 40°C	Lachnoclostridium phytofermentans
42	-	2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose	phosphorolysis, pH 7, 40°C	Lachnoclostridium phytofermentans
46	-	2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose	synthesis, pH 7, 40°C	Lachnoclostridium phytofermentans
47	-	2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose	phosphorolysis, pH 7, 40°C	Lachnoclostridium phytofermentans
48	-	2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose	phosphorolysis, pH 7, 40°C	Lachnoclostridium phytofermentans
55	-	2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose	synthesis, pH 7, 40°C	Lachnoclostridium phytofermentans
63	-	phosphate	pH 7, 40°C	Lachnoclostridium phytofermentans

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	-	Lachnoclostridium phytofermentans

pH Range

pH Minimum	pH Maximum	Comment	Organism
6.5	8	more than 80% of maximum activity	Lachnoclostridium phytofermentans

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
5.7	-	alpha-D-glucose 1-phosphate	pH 7, 40°C	Lachnoclostridium phytofermentans
7.3	-	2-O-beta-D-glucopyranosyl-D-glucopyranose	synthesis, pH 7, 40°C	Lachnoclostridium phytofermentans
9.8	-	2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose	phosphorolysis, pH 7, 40°C	Lachnoclostridium phytofermentans
11	-	alpha-D-glucose 1-phosphate	pH 7, 40°C	Lachnoclostridium phytofermentans
19	-	2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose	phosphorolysis, pH 7, 40°C	Lachnoclostridium phytofermentans
20	-	2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose	synthesis, pH 7, 40°C	Lachnoclostridium phytofermentans
21	-	2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose	synthesis, pH 7, 40°C	Lachnoclostridium phytofermentans
23	-	2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose	phosphorolysis, pH 7, 40°C	Lachnoclostridium phytofermentans

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Release 2023.1 (January 2023)